Rabbit anti-Human HIST1H2AG Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00156A

Western Blot | Positive WB detected in: NIH/3T3 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate | All lanes: HIST1H2AG antibody at 1µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 15 kDa | Observed band size: 15 kDa

Immunoprecipitating HIST1H2AG in NIH/3T3 whole cell lysate | Lane 1: Rabbit control IgG instead of HIST1H2AG (Ab-118) Antibody in NIH/3T3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: HIST1H2AG (Ab-118) Antibody (5µg) + NIH/3T3 whole cell lysate (500µg) | Lane 3: NIH/3T3 whole cell lysate (20µg)

Chromatin Immunoprecipitation Hela (106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2AG (HIST1H2AG (Ab-118) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Rabbit anti-Human HIST1H2AG Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00156A
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Product Overview
Product Title | Rabbit anti-Human HIST1H2AG Polyclonal Antibody |
Description | The antibody against HIST1H2AG was raised in rabbit using the Peptide sequence around site of Lys (118) derived from Human Histone H2A type 1 as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IP, ChIP. |
Uniprot Id - Alink | P0C0S8 |
Host Species | Rabbit |
Reactivity | Human, Mouse |
Target Name | HIST1H2AG |
Target Synonyms | H2AC11 antibody; H2AFP antibody; HIST1H2AG; antibody; H2AC13 antibody; H2AFC antibody; HIST1H2AI; antibody; H2AC15 antibody; H2AFD antibody; HIST1H2AK; antibody; H2AC16 antibody; H2AFI antibody; HIST1H2AL; antibody; H2AC17 antibody; H2AFN antibody; HIST1H2AMHistone H2A type 1 antibody; H2A.1 antibody; Histone H2A/ptl antibody |
Immunogen Description | Peptide sequence around site of Lys (118) derived from Human Histone H2A type 1 |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Polyclonal |
Isotype | IgG |
Purification Method | Antigen affinity purified |
Buffer | 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Form | Liquid |
Application | ELISA, WB, IP, ChIP |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |