Rabbit anti-Human HIST1H2AG Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00154A

Western Blot | Detected samples: Hela whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h | All lanes: HIST1H2AG antibody at 1:100 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 15 kDa | Observed band size: 15 kDa

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with Acetyl-HIST1H2AG (K74) Antibody at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunocytochemistry analysis of Acetyl-HIST1H2AG (K74) Antibody diluted at 1:5 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Rabbit anti-Human HIST1H2AG Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00154A
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Product Overview
Product Title | Rabbit anti-Human HIST1H2AG Polyclonal Antibody |
Description | The antibody against HIST1H2AG was raised in rabbit using the Peptide sequence around site of Acetyl-Lys (74) derived from Human Histone H2A type 1 as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, ICC, IF. |
Uniprot Id - Alink | P0C0S8 |
Host Species | Rabbit |
Reactivity | Human |
Target Name | HIST1H2AG |
Target Synonyms | H2AC11 antibody; H2AFP antibody; HIST1H2AG; antibody; H2AC13 antibody; H2AFC antibody; HIST1H2AI; antibody; H2AC15 antibody; H2AFD antibody; HIST1H2AK; antibody; H2AC16 antibody; H2AFI antibody; HIST1H2AL; antibody; H2AC17 antibody; H2AFN antibody; HIST1H2AMHistone H2A type 1 antibody; H2A.1 antibody; Histone H2A/ptl antibody |
Immunogen Description | Peptide sequence around site of Acetyl-Lys (74) derived from Human Histone H2A type 1 |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Polyclonal |
Isotype | IgG |
Purification Method | Antigen affinity purified |
Buffer | 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Form | Liquid |
Application | ELISA, WB, ICC, IF |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |