Rabbit anti-Human HIST1H1C Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00084A

Western Blot | Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h | All lanes: HIST1H1C antibody at 2.6µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 22 kDa | Observed band size: 22 kDa

Immunocytochemistry analysis of Acetyl-HIST1H1C (K84) Antibody diluted at 1:5 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1C (Acetyl-HIST1H1C (K84) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Rabbit anti-Human HIST1H1C Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00084A
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Product Overview
Product Title | Rabbit anti-Human HIST1H1C Polyclonal Antibody |
Description | The antibody against HIST1H1C was raised in rabbit using the Peptide sequence around site of Acetyl-Lys (84) derived from Human Histone H1.2 as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, ICC, ChIP. |
Uniprot Id - Alink | P16403 |
Host Species | Rabbit |
Reactivity | Human |
Target Name | HIST1H1C |
Target Synonyms | H1 histone family member 2 antibody; H1.a antibody; H12_HUMAN antibody; H1F2 antibody; H1s-1 antibody; HIST1H1C antibody; Histone 1 H1c antibody; Histone cluster 1 H1c antibody; Histone H1.2 antibody; Histone H1c antibody; Histone H1d antibody; Histone H1s-1 antibody; MGC3992 antibody |
Immunogen Description | Peptide sequence around site of Acetyl-Lys (84) derived from Human Histone H1.2 |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Polyclonal |
Isotype | IgG |
Purification Method | Antigen affinity purified |
Buffer | 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Form | Liquid |
Application | ELISA, WB, ICC, ChIP |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |