Rabbit anti-Human GNAS Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-02535A
Western Blot  | Positive WB detected in: Rat brain tissue, Mouse brain tissue  | All lanes: GNAS antibody at 3µg/ml  | Secondary  | Goat polyclonal to rabbit IgG at 1/50000 dilution  | Predicted band size: 46, 45, 112, 110, 78, 29 kDa  | Observed band size: 46 kDa
Western Blot | Positive WB detected in: Rat brain tissue, Mouse brain tissue | All lanes: GNAS antibody at 3µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 46, 45, 112, 110, 78, 29 kDa | Observed band size: 46 kDa
Immunohistochemistry of paraffin-embedded human brain tissue using GNAS Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using GNAS Antibody at dilution of 1:100
Immunofluorescent analysis of MCF-7 cells using GNAS Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of MCF-7 cells using GNAS Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Rabbit anti-Human GNAS Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-02535A
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Product Overview

Product Title Rabbit anti-Human GNAS Polyclonal Antibody
Description The antibody against GNAS was raised in rabbit using the Recombinant Human Guanine nucleotide-binding protein G(s) subunit alpha isoforms short protein (1-394AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IF.
Uniprot Id - Alink P63092
Host Species Rabbit
Reactivity Human, Mouse, Rat
Target Name GNAS
Target Synonyms Adenylate cyclase-stimulating G alpha protein antibody; AHO antibody; alternative gene product encoded by XL-exon antibody; C20orf45 antibody; extra large alphas protein antibody; GNAS antibody; GNAS complex locus antibody; GNAS1 antibody; GNAS1 gene, formerly antibody; GNAS1, formerly antibody; GNAS2_HUMAN antibody; GPSA antibody; GSA antibody; GSP antibody; guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 antibody; guanine nucleotide regulatory protein antibody; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short antibody; guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas antibody; NESP antibody; neuroendocrine secretory protein antibody; PHP1A antibody; PHP1B antibody; PHP1C antibody; POH antibody; protein ALEX antibody; secretogranin VI antibody
Immunogen Description Recombinant Human Guanine nucleotide-binding protein G(s) subunit alpha isoforms short protein (1-394AA)
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Buffer 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Application ELISA, WB, IHC, IF
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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