Rabbit anti-Human ERBB3 Polyclonal Antibody

Name: Rabbit anti-Human ERBB3 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-05549A
Price: 299.0 USD
Availability: InStock

Western blot analysis of extracts from A431 cells untreated or treated with EGF using Her3/ErbB3(phospho-Tyr1328) Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Her3/ErbB3(Phospho-Tyr1328) Antibody(left) or the same antibody preincubated with blocking peptide(right).

Immunofluorescence staining of methanol-fixed MCF7 cells using Her3/ErbB3(phospho-Tyr1328) Antibody.

Collections: All products, Back to school. back to lab. ready for results. - advance your research with beta lifescience, Buy antibodies for advanced research

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Product Title	Rabbit anti-Human ERBB3 Polyclonal Antibody
Description	The antibody against ERBB3 was raised in rabbit using the Peptide sequence around phosphorylation site of tyrosine 1328 (P-D-Y(p)-W-H) derived from Human Her3/ErbB3. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB, IHC, IF.
Uniprot Id - Alink	P21860
Host Species	Rabbit
Reactivity	Human, Mouse, Rat
Target Name	ERBB3
Target Synonyms	c erbB 3 antibody; c erbB3 antibody; Erb b2 receptor tyrosine kinase 3 antibody; ErbB 3 antibody; ERBB3 antibody; ERBB3 protein antibody; erbB3 S antibody; ERBB3_HUMAN antibody; Glial growth factor receptor antibody; HER 3 antibody; HER3 antibody; Human epidermal growth factor receptor 3 antibody; LCCS2 antibody; MDA BF 1 antibody; MGC88033 antibody; p180 ErbB3 antibody; p45 sErbB3 antibody; p85 sErbB3 antibody; proto-oncogene-like protein c ErbB 3 antibody; proto-oncogene-like protein c ErbB3 antibody; Proto-oncogene-like protein c-ErbB-3 antibody; Receptor tyrosine protein kinase erbB 3 antibody; Receptor tyrosine protein kinase erbB3 antibody; Receptor tyrosine-protein kinase erbB-3 antibody; Tyrosine kinase type cell surface receptor HER3 antibody; Tyrosine kinase-type cell surface receptor HER3 antibody; v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody; v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody; v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
Immunogen Description	Peptide sequence around phosphorylation site of tyrosine 1328 (P-D-Y(p)-W-H) derived from Human Her3/ErbB3.
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Clonality	Polyclonal
Purification Method	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Liquid
Application	ELISA, WB, IHC, IF
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.