Rabbit anti-Human EIF4E Polyclonal Antibody

Name: Rabbit anti-Human EIF4E Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-05498A
Price: 299.0 USD
Availability: InStock

Western blot analysis of extracts from Hela, HepG2 and 293 cells using eIF4E(Ab-209) Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E(Ab-209) Antibody(left) or the same antibody preincubated with blocking peptide(right).

Immunofluorescence staining of methanol-fixed Hela cells using eIF4E(Ab-209) Antibody.

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Product Overview

Product Title	Rabbit anti-Human EIF4E Polyclonal Antibody
Description	The antibody against EIF4E was raised in rabbit using the Peptide sequence around aa. 207~211 (S-G-S-T-T) derived from Human eIF4E. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. This antibody has been validated on ELISA, WB, IHC, IF.
Uniprot Id - Alink	P06730
Host Species	Rabbit
Reactivity	Human, Mouse, Rat
Target Name	EIF4E
Target Synonyms	AUTS19 antibody; CBP antibody; eIF 4E antibody; eIF 4F 25 kDa subunit antibody; EIF 4F antibody; eIF-4E antibody; eIF-4F 25 kDa subunit antibody; eIF4E antibody; EIF4E1 antibody; EIF4EL1 antibody; EIF4F antibody; Eukaryotic translation initiation factor 4 E antibody; Eukaryotic translation initiation factor 4E antibody; Eukaryotic translation initiation factor 4E like 1 antibody; IF4E_HUMAN antibody; Messanger RNA Cap Binding Protein eIF 4E antibody; MGC111573 antibody; mRNA cap binding protein antibody; mRNA cap-binding protein antibody
Immunogen Description	Peptide sequence around aa. 207~211 (S-G-S-T-T) derived from Human eIF4E.
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Clonality	Polyclonal
Purification Method	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Liquid
Application	ELISA, WB, IHC, IF
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.