Rabbit anti-Human EIF2AK1 Polyclonal Antibody

Name: Rabbit anti-Human EIF2AK1 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-01157A
Price: 299.0 USD
Availability: InStock

Western BlotPositive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Rat heart tissue, Rat lung tissueAll lanes: EIF2AK1 antibody at 3μg/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 72 kDaObserved band size: 72 kDa

Immunohistochemistry of paraffin-embedded human spleen tissue using EIF2AK1 Antibody at dilution of 1:100

Immunohistochemistry of paraffin-embedded human testis tissue using EIF2AK1 Antibody at dilution of 1:100

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Product Overview

Product Title	Rabbit anti-Human EIF2AK1 Polyclonal Antibody
Description	The antibody against EIF2AK1 was raised in rabbit using the Recombinant Human Eukaryotic translation initiation factor 2-alpha kinase 1 protein (1-86AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IF.
Uniprot Id - Alink	Q9BQI3
Host Species	Rabbit
Reactivity	Human, Rat
Target Name	EIF2AK1
Target Synonyms	E2AK1_HUMAN antibody; EC 2.7.11.1 antibody; Eif2ak1 antibody; Eukaryotic translation initiation factor 2 alpha kinase 1 antibody; Eukaryotic translation initiation factor 2-alpha kinase 1 antibody; HCR antibody; Heme controlled repressor antibody; Heme regulated eukaryotic initiation factor eIF 2 alpha kinase antibody; Heme regulated inhibitor antibody; Heme regulated initiation factor 2 alpha kinase antibody; Heme regulated repressor antibody; Heme sensitive initiation factor 2a kinase antibody; Heme-controlled repressor antibody; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase antibody; Heme-regulated inhibitor antibody; Hemin sensitive initiation factor 2 alpha kinase antibody; Hemin-sensitive initiation factor 2-alpha kinase antibody; HRI antibody; KIAA1369 antibody; PRO1362 antibody
Immunogen Description	Recombinant Human Eukaryotic translation initiation factor 2-alpha kinase 1 protein (1-86AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	>95%, Protein G purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form	Liquid
Application	ELISA, WB, IHC, IF
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.