Mouse anti-Human CD14 Monoclonal antibody

Name: Mouse anti-Human CD14 Monoclonal antibody
Brand: Beta LifeScience
SKU: BLC-06422A
Price: 299.0 USD
Availability: InStock

Western BlotPositive WB detected in: Rabbit spleen tissue, Rabbit small intestine tissueAll lanes: CD14 antibody at 1:2500SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 41 kDaObserved band size: 55 kDa

Western BlotPositive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysateAll lanes: CD14 antibody at 1:1800SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 41 kDaObserved band size: 55 kDa

Immunofluorescence staining of A549 cells with CD14 Monoclonal Antibody at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Product Overview

Product Title	Mouse anti-Human CD14 Monoclonal antibody
Description	The antibody against CD14 was raised in mouse using the Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG1, purified by protein A with a purity greater than 95%. This antibody has been validated on ELISA, WB, IF, FC.
Uniprot Id - Alink	P08571
Host Species	Mouse
Reactivity	Human, Mouse, Rabbit
Target Name	CD14
Target Synonyms	CD 14 antibody; CD_antigen=CD14 antibody; CD14 antibody; CD14 antigen antibody; CD14 molecule antibody; CD14_HUMAN antibody; LPS-R antibody; Mo2 antibody; Monocyte differentiation antigen CD14 antibody; Monocyte differentiation antigen CD14 urinary form antibody; Monocyte differentiation antigen CD14; membrane-bound form antibody; Myeloid cell specific leucine rich glycoprotein antibody; Myeloid cell-specific leucine-rich glycoprotein antibody
Immunogen Description	Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Monoclonal
Isotype	IgG1
Purification Method	>95%, Protein A purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form	Liquid
Application	ELISA, WB, IF, FC
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.