Mouse anti-Human ABCB1 Monoclonal antibody

Beta LifeScience SKU/CAT #: BLC-06398A
Western Blot Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate All lanes: ABCB1 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 141 kDa Observed band size: 141-250 KDa Exposure time：5min
Western Blot Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate All lanes: ABCB1 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 141 kDa Observed band size: 141-250 KDa Exposure time：5min
Immunofluorescence staining of HepG2 cells with ABCB1 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescence staining of HepG2 cells with ABCB1 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescence staining of Hela cells with ABCB1 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescence staining of Hela cells with ABCB1 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Western Blot Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate All lanes: ABCB1 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 141 kDa Observed band size: 141-250 KDa Exposure time：5min
Immunofluorescence staining of HepG2 cells with ABCB1 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescence staining of Hela cells with ABCB1 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Mouse anti-Human ABCB1 Monoclonal antibody

Beta LifeScience SKU/CAT #: BLC-06398A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Product Title Mouse anti-Human ABCB1 Monoclonal antibody
Description The antibody against ABCB1 was raised in mouse using the Recombinant Human Calnexin protein (350-710AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG2b, purified by protein A with a purity greater than 95%. This antibody has been validated on ELISA, WB, IF, FC.
Uniprot Id - Alink Mouse
Host Species Mouse
Reactivity Human
Target Name ABCB1
Target Synonyms ABC20 antibody; ABCB1 antibody; ATP binding cassette, sub family B (MDR/TAP), member 1 antibody; ATP-binding cassette sub-family B member 1 antibody; CD243 antibody; CLCS antibody; Colchicin sensitivity antibody; Doxorubicin resistance antibody; GP170 antibody; MDR1 antibody; MDR1_HUMAN antibody; Multidrug resistance 1 antibody; Multidrug resistance protein 1 antibody; P glycoprotein 1 antibody; P gp antibody; P-glycoprotein 1 antibody; PGY1 antibody
Immunogen Description Recombinant Human Calnexin protein (350-710AA)
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG2b
Purification Method >95%, Protein A purified
Buffer 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Application ELISA, WB, IF, FC
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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