Recombinant Cynomolgus Kallikrein 5/KLK5 Protein

Name: Recombinant Cynomolgus Kallikrein 5/KLK5 Protein, Active
Brand: Beta LifeScience
SKU: BLK-03003P
Availability: InStock

Cynomolgus Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Collections: All products, High-quality recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	Recombinant Cynomolgus Kallikrein 5/KLK5 Protein (active form) is expressed from HEK293 with His tag at the C-terminus.It contains Ile65-Ser291.
Gene Background	The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.
Accession	XP_005590112.3
Target Symbol	Kallikrein 5/KLK5
Synonyms	Kallikrein c; Klnc; KLK5; KLKL2; KLK-L2; SCTE
Species	Cynomolgus
Expression System	Mammalian Cell
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Tag	C-His
Expression Range	Ile65-Ser291
Mol. Weight	The protein has a predicted MW of 26.64 kDa. Due to glycosylation, the protein migrates to 38-52 kDa based on Bis-Tris PAGE result.
Form	Lyophilized
Formulation	Lyophilized from 0.22 um filtered solution in 20mM NaAc, 150mM NaCl, 8% trehalose (pH 5.0).
Endotoxin	Less than 1 EU per ug by the LAL method.
Activity	Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >500 pmol/min/µg.
Biotinylated	No
Shipping	Shipped at ambient temperature.
Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution	Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Cynomolgus Kallikrein 5/KLK5 Protein, Active

Recombinant Cynomolgus Kallikrein 5/KLK5 Protein, Active

Product Overview

FAQs