ACTA1 Recombinant Monoclonal Antibody

Beta LifeScience SKU/CAT #: BLC-06499A
Western BlotPositive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Raw264.7 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat heart tissueAll lanes: Actin antibody at 0.95μg/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDa
Western BlotPositive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Raw264.7 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat heart tissueAll lanes: Actin antibody at 0.95μg/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDa
IHC image of ACTA1 Recombinant Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of ACTA1 Recombinant Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with ACTA1 Recombinant Monoclonal Antibody at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunofluorescence staining of HepG2 cells with ACTA1 Recombinant Monoclonal Antibody at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Western BlotPositive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Raw264.7 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat heart tissueAll lanes: Actin antibody at 0.95μg/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDa
IHC image of ACTA1 Recombinant Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with ACTA1 Recombinant Monoclonal Antibody at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ACTA1 Recombinant Monoclonal Antibody

Beta LifeScience SKU/CAT #: BLC-06499A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Product Title ACTA1 Recombinant Monoclonal Antibody
Description The recombinant antibody against ACTA1 was produced using a synthesized peptide as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IHC, IF, FC.
Uniprot Id - Alink P68133
Reactivity Human, Mouse, Rat
Target Name ACTA1
Target Synonyms a actin antibody; ACTA antibody; ACTA1 antibody; ACTA2 antibody; ACTC antibody; ACTC1 antibody; Actin antibody; ACTS_HUMAN antibody; ACTSA antibody; Alpha 2 actin antibody; alpha skeletal muscle antibody; Alpha-actin-1 antibody; Cardiac muscle alpha actin 1 antibody; Skeletal muscle alpha actin 1 antibody
Immunogen Description A synthesized peptide
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography purified
Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Application ELISA, WB, IHC, IF, FC
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Immunocytochemistry analysis of Acetyl-Histone H4 (K16) Recombinant Monoclonal Antibody diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunocytochemistry analysis of Acetyl-Histone H4 (K16) Recombinant Monoclonal Antibody diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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