IL23 P19 Protein, Marmoset (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2845
IL23 P19 Protein, Marmoset (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2845
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Product Overview
Tag | His |
Host Species | Marmoset |
Background | IL-23, which is mainly secreted by antigen-presenting cells, is a member of the IL-12 family, which includes IL-12, IL-27, and IL-35[1]. IL-23 is a heterodimeric cytokine, comprised a unique p19 subunit and p4 subunit, the latter of which is shared with IL-12. The receptor for IL-23 consists of IL-23R and IL-12Rbeta1, the latter of which is also characteristic of IL-12. IL-23 is essential for Th17 differentiation, expansion, and survival by binding to its receptor, thereby activating the signaling pathway [11,12]. Many studies revealed that the IL-23/Th17 pathway is implicated in the pathophysiology of various autoimmune diseases, such as autoimmune arthritis[13], primary biliary cirrhosis[14], and inflammatory bowel disease[15].Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
Description | A DNA sequence encoding the marmoset IL23A (Met 1-Pro 189) was fused with a His tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the marmoset IL12B (Met 1-Asn328) was expressed with a His tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the marmoset IL23A & the marmoset IL12B heterodimer was purified. |
Source | HEK293 |
Predicted N Terminal | Arg 20 & Ile 23 |
AA Sequence | Met 1-Pro 189 |
Molecular Weight | The recombinant marmoset IL23A & the marmoset IL12B comprises 498(181+317) a.a. and has a calculated molecular mass of 56.4 (20.1+36.3) KDa. The apparent molecular mass of the marmoset IL23A & the marmoset IL12B heterodimer is approximately 22 & 45 KDa respectively in SDS-PAGE. |
Purity | >85% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its binding ability in a functional ELISA.Immobilized marmoset IL23A-His+marmoset IL12B-His at 10 ug/ml (100 ul/well) can bind biotinylated human IL12RB1-His. The EC50 of biotinylated human IL12RB1-His is 55-129 ug/ml. |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |