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IHC image of the antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of K562 cells with Phospho-RB1 (S780) Recombinant Monoclonal Antibody at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Phospho-RB1 (S780) Recombinant Monoclonal Antibody

CAT#: BLC-06479A

Western blot analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser780) Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser780) Antibody(left) or the same antibody preincubated with blocking peptide(right).

Rabbit anti-Human RB1 Polyclonal Antibody

CAT#: BLC-05602A

Western blot analysis of extracts from K562 cells untreated(lane 1) or treated with serum(lane 2) using Rb(Phospho-Ser795) Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser795) Antibody(left) or the same antibody preincubated with blocking peptide(right).

Rabbit anti-Human RB1 Polyclonal Antibody

CAT#: BLC-05603A

Greater than 90% as determined by SDS-PAGE.

Recombinant Rat Retinoblastoma-Associated Protein (RB1) Protein (His)

Molecule: Rb1;
Species: Rat;
Expression system: E.coli;
Tag: His;
Sequence: Protein Fragment;

CAT#: BLC-08268P

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