Phospho-RB1 (S780) Recombinant Monoclonal Antibody

Beta LifeScience SKU/CAT #: BLC-06479A
IHC image of the antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of the antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of K562 cells with Phospho-RB1 (S780) Recombinant Monoclonal Antibody at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunofluorescence staining of K562 cells with Phospho-RB1 (S780) Recombinant Monoclonal Antibody at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating Phospho-RB1 in Hela whole cell lysate | Lane 1: Rabbit control IgG(1μg)instead of Phospho-RB1 (S780) Recombinant Monoclonal Antibody in Hela whole cell lysate. | For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: Phospho-RB1 (S780) Recombinant Monoclonal Antibody(3μg)+ Hela whole cell lysate(1mg) | Lane 3: Hela whole cell lysate (20μg)
Immunoprecipitating Phospho-RB1 in Hela whole cell lysate | Lane 1: Rabbit control IgG(1μg)instead of Phospho-RB1 (S780) Recombinant Monoclonal Antibody in Hela whole cell lysate. | For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: Phospho-RB1 (S780) Recombinant Monoclonal Antibody(3μg)+ Hela whole cell lysate(1mg) | Lane 3: Hela whole cell lysate (20μg)
IHC image of the antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of K562 cells with Phospho-RB1 (S780) Recombinant Monoclonal Antibody at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating Phospho-RB1 in Hela whole cell lysate | Lane 1: Rabbit control IgG(1μg)instead of Phospho-RB1 (S780) Recombinant Monoclonal Antibody in Hela whole cell lysate. | For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: Phospho-RB1 (S780) Recombinant Monoclonal Antibody(3μg)+ Hela whole cell lysate(1mg) | Lane 3: Hela whole cell lysate (20μg)

Phospho-RB1 (S780) Recombinant Monoclonal Antibody

Beta LifeScience SKU/CAT #: BLC-06479A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Product Title Phospho-RB1 (S780) Recombinant Monoclonal Antibody
Description The recombinant antibody against RB1 was produced using a synthesized peptide derived from Human Phospho-RB1 (S780) as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, IHC, IF, IP.
Uniprot Id - Alink P06400
Reactivity Human
Target Name RB1
Target Synonyms Exon 17 tumor GOS561 substitution mutation causes premature stop antibody; GOS563 exon 17 substitution mutation causes premature stop antibody; OSRC antibody; Osteosarcoma antibody; p105-Rb antibody; P105RB antibody; PP105 antibody; pp110 antibody; PPP1R130 antibody; pRb antibody; Prepro retinoblastoma associated protein antibody; Protein phosphatase 1 regulatory subunit 130 antibody; Rb antibody; RB transcriptional corepressor 1 antibody; RB_HUMAN antibody; RB1 antibody; RB1 gene antibody; Retinoblastoma 1 antibody; Retinoblastoma suspectibility protein antibody; Retinoblastoma-associated protein antibody
Immunogen Description A synthesized peptide derived from Human Phospho-RB1 (S780)
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography purified
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Application ELISA, IHC, IF, IP
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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