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The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using the antibody Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using NPHS1 Antibody(NPHS1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)

Rabbit anti-Human NPHS1 Polyclonal Antibody

CAT#: BLC-03649A

IHC image of the antibody diluted at 1:250 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot | Positive WB detected in: Jurkat whole cell lysate | All lanes: NPHS1 antibody at 5.6µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 135, 131 kDa | Observed band size: 135 kDa

Rabbit anti-Human NPHS1 Polyclonal Antibody

CAT#: BLC-05969A

Greater than 90% as determined by SDS-PAGE.

Recombinant Human Nephrin (NPHS1) Protein (His)

Molecule: NPHS1;
Species: Human;
Expression system: E.coli;
Tag: His;
Sequence: Protein Fragment;

CAT#: BLC-03160P

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Greater than 85% as determined by SDS-PAGE.

Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) NPHS1.

Recombinant Human Nephrin (NPHS1) Protein (His)

Molecule: NPHS1;
Species: Human;
Expression system: Mammalian Cell;
Tag: His;
Sequence: Protein Fragment;

CAT#: BLC-09124P

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