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SDS-PAGE analysis of Human KRT20 (Keratin, Type I Cytoskeletal 20) - Recombinant Protein, CAT# BLT-01665P, showing >85% purity under 15% SDS-PAGE (Reduced)

Human KRT20 (Keratin, Type I Cytoskeletal 20) - Recombinant Protein

Molecule: KRT20;
Species: Human;
Expression system: E.coli;
Tag: N-6His;
Sequence: 9-424aa;

CAT#: BLT-01665P

IHC image of the antibody diluted at 1:300 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with KRT20 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit anti-Human KRT20 Polyclonal Antibody

CAT#: BLC-00042A

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 20 antibody.

Western blot analysis of extracts from HeLa cells, using Keratin 20 antibody.

Rabbit anti-Human KRT20 Polyclonal Antibody

CAT#: BLC-05328A

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