Recombinant Mouse Wnt3a Protein

Collections: Cytokines and growth factors, Other cytokines, Recombinant proteins

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Product Overview

Host Species	Mouse
Accession	P27467
Synonym	Protein Wnt 3a Precursor Protein Wnt-3a Wingless type MMTV integration site family member 3A Wnt 3a wnt3a WNT3A protein WNT3A_HUMAN
Description	Recombinant Mouse Wnt3a Protein was expressed in Cell Culture. It is a Full length protein
Source	Cell Culture
AA Sequence	SY PIWWSLAVGP QYSSLSTQPI LCASIPGLVP KQLRFCRNYV EIMPSVAEGV KAGIQECQHQ FRGRRWNCTT VSNSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GSAAICGCSS RLQGSPGEGW KWGGCSEDIE FGGMVSREFA DARENRPDAR SAMNRHNNEA GRQAIASHMH LKCKCHGLSG SCEVKTCWWS QPDFRTIGDF LKDKYDSASE MVVEKHRESR GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS SHGIDGCDLL CCGRGHNART ERRREKCHCV FHWCCYVSCQ ECTRVYDVHT CK
Purity	>75% SDS-PAGE.Greater than 75% by HPLC analyses.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells.The expected ED50 for this effect is 0.4-0.6 ng/ml.
Formulation	Lyophilised
Stability	The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution	See related COA
Unit Definition	For Research Use Only
Storage Buffer	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Target Details

Target Function	Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).
Subcellular Location	Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families	Wnt family
Database References	KEGG: mmu:22416 STRING: 10090.ENSMUSP00000010044 UniGene: Mm.1367
Tissue Specificity	Dorsal portion of the neural tube (developing roof plate), and mesenchyme tissue surrounding the umbilical veins.

Gene Functions References

This work provides a new link between Wnt3a and macrophage-mediated angiogenesis under glucose and oxygen deprivation in vitro. PMID: 29199847
Lrp6 is the key mediator of Wnt3a signaling in osteoblasts and Lrp5 played a less significant role in mediating Wnt3a signaling. PMID: 29176883
These data define the mechanism responsible for the repressive effects of nitric oxide (NO) on the transcriptional activity of beta-catenin and link eNOS-derived NO to the modulation by VEGF of Wnt/beta-catenin-induced endothelial cell proliferation. PMID: 28320874
These results suggest novel mechanisms for Wnt3a-induced osteoblast proliferation and cell survival via Npnt gene expression PMID: 28093227
this study shows that the Wnt3a expression levels in dendritic cells influences the generation of memory T cells after 5 days in co-culture with naive T cells through activation of the Wnt canonical pathway PMID: 27544306
Wnt3a induces Osx expression via p38 MAPK signaling in dental follicle cells. Wnt3a-induced Osx expression was inhibited in the presence of p38 mitogen-activated protein kinase (MAPK) inhibitors (SB203580 and SB202190) at gene and protein levels, as assessed by real-time PCR and immunocytohistochemistry, respectively. PMID: 27450807
Western blot analysis demonstrated that following BMP2 and BMP7 cotransfection of MC3T3E1 cells, the protein expression levels of BMP2, BMP4, BMP6, BMP7, BMP9 and Wnt3a were increased compared with control cells PMID: 27633082
Wnt3a promotes macrophage-mediated bacterial killing by elevating CRAMP and BD1 levels. PMID: 26846714
Wnt3a acutely reduces nuclear acetyl-CoA, the necessary substrate for histone acetyltransferases, resulting in a global decrease in histone acetylation. PMID: 27129247
Wnt3a rapidly activated the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway and pharmacological stimulation of cAMP/PKA signaling suppressed osteoclast differentiation PMID: 26189772
Mesenchymal Stem Cells Within Gelatin/CaSO4 Scaffolds Treated Ex Vivo with Low Doses of BMP-2 and Wnt3a Increase Bone Regeneration. PMID: 26414873
Wnt3a increased the expression of NeuroD1 and Ins2 in the hypothalamus. PMID: 26956881
that activation of the Wnt pathway made cells more readily tolerate ROS-caused mitochondria injury and cell apoptosis PMID: 26085341
Pin1 is indispensable for Wnt3a-induced osteoblast differentiation. PMID: 26740630
The mouse xenograft model showed that Wnt3a-overexpressing cells grew into larger tumor masses and formed more vasculogenic mimicry than the control cells. PMID: 26266404
miR-27-3p inhibits melanogenesis by repressing Wnt3a protein expression. PMID: 26006230
Wnt8a and Wnt3a cooperate to maintain Fgf8 expression and prevent premature Sox2 up-regulation in the axial stem cell niche, critical for posterior growth. PMID: 25809880
WNT3A promotes myogenesis of human embryonic stem cells and enhances in vivo heterologous engraftment. PMID: 25084050
C1q-induced activation of Wnt3a/CTNNB1/FOXO1 signaling contributes to skeletal fiber type shift toward fatigable fiber in congestive heart failure. PMID: 26038536
Study reports that NRF2 participates in the formation of a protein complex with Axin1/GSK-3/beta-TrCP that is disrupted by WNT-3A, a prototypic canonical WNT ligand, and leads to upregulation of the NRF2 transcriptional signature. PMID: 25336178
Wnt3a and MMP9 induce C3H10T1/2 cells differentiation into cardiomyocyte-like cells. PMID: 25854560
Wnt3a/beta-catenin signaling directs trunk progenitors towards paraxial mesodermal progenitor fates; however, our data also suggest that Wnt3a positively supports a progenitor state for both mesodermal and neural progenitors. PMID: 25922526
the presented systems-biology approach demonstrates that Wnt3a attenuates RANKL-driven osteoclastogenesis by blocking c-Fos expression PMID: 25038457
Wnt3a/Frizzled-3 signaling plays important role in regulating the proliferation and differentiation of neural crest cells and various developmental stages of melanocyte precursors. PMID: 24815018
Sp5 and Sp8 are pivotal downstream effectors of Wnt3a/beta-catenin that are essential for the self-renewal and differentiation of neuromesodermal stem cells. PMID: 24475213
results suggest that Wnt3a overrides the effect of P2X7R on the Wnt/beta-catenin signaling to prevent the AEC I death and restrict the severity of ALI. PMID: 24922070
these data identify HES5 as a key mediator of the Wnt-3a proneurogenic effect occurring independently of the classical Wnt/beta-catenin signaling cascade thus further deciphering crosstalk mechanisms of Wnt and Notch signaling pathways regulating cell fate PMID: 24548083
These results indicate that JBP regulates the expressions of SHP-1, Wnt3a, and AP-1 proteins in chemically damaged mice. PMID: 24841652
our results suggest a dose-dependent role for Wnt3a in regulating the balance between renewal and selection of differentiation fates of axial progenitors in the epiblast. PMID: 25152336
Mutation of any individual cysteine of Wnt3a results in covalent Wnt oligomers through ectopic intermolecular disulfide bond formation and diminishes/abolishes Wnt signaling. PMID: 24841207
epigenetic activation of Bmp2 gene expression contributes to Wnt3a-mediated direct trans-differentiation of pre-adipocytes or fibroblasts into osteoblasts PMID: 24867947
Increasing amounts of soluble Frizzled8-cysteine-rich-domain protein modulated Wnt3a signaling in a biphasic manner. PMID: 24885675
results demonstrate that, while ER signaling acts synergistically with Wnt3A in promoting osteogenic differentiation, Wnt3A may crosstalk with ER signaling by up-regulating ERalpha expression and down-regulating ERbeta expression in MPCs. PMID: 24340027
Wnt3a/beta-catenin signaling regulates follicular development by modulating the expression of Foxo3a signaling components. PMID: 24246780
Smad4 regulates maturation of skeletal collagen and osteoblast survival, and is required for matrix-forming responses to both BMP2 and Wnt3a. PMID: 24006258
Jianpi Bushen Prescription plays a protective role on radiation-induced bone marrow through the activation of the Wnt3a signaling pathway. PMID: 24114209
Structure-based mutational analysis of mouse Wnt3a shows that the linker between the N- and C-terminal domains is required for LRP6 binding. PMID: 23791946
Results demonstrated that Wnt3a regulates proliferation, apoptosis and enhances function of pancreatic NIT-1 beta cells via activation of Wnt/beta-catenin signaling. PMID: 23296977
Data indicate that Wnt/beta-catenin signaling mediates nandrolone-induced Numb transcription. PMID: 23649620
WNTs have a dual and context-dependent effect on microglia acting in a homeostatic pro- and anti-inflammatory manner. PMID: 23534675
We identified a number of stimuli triggering Wnt/beta-catenin pathway activation, including exogenous NKT cell activator, glycolipid alpha-GalCer, and endogenous prostaglandin E2 (PGE2). PMID: 22991247
Results support a potential mechanism for the differentiation of mesenchymal stem cells into type II alveolar cells involving canonical wnt pathway activation. PMID: 23154940
Wnt3a is a putative molecular partner of Zbtb20 in translating early positional information for the establishment of all developing medial pallidum fields. PMID: 22689450
findings suggest that a spatially restricted Wnt signal induces an oriented cell division in embryonic stem cells that generates distinct cell fates at predictable positions relative to the Wnt source PMID: 23520113
Wnt3a stimulates repaid translocation of IPMK to the cell membrane. PMID: 22940627
results identify LRP8 as a novel positive factor of canonical Wnt signaling pathway and show its involvement in Wnt-induced osteoblast differentiation PMID: 22589174
results of this study suggest that Wnt3a selectively stimulates proliferation of progenitors that are committed to differentiation or are in the process of exiting the SSC state, leading to enhanced formation of germ cell communities PMID: 22761943
Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2. PMID: 22357953
Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells. Wnt3a plays an important role in melanocyte homeostasis. PMID: 22710324
These data suggest that stabilization of beta-catenin by WNT3A inhibits BMP2-mediated induction of cementoblast/osteoblast differentiation in SVF4 cells, although BMP2 requires endogenous Wnt/beta-catenin signaling to promote cell maturation. PMID: 22150562

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.