Recombinant Human SIRPA Protein

Collections: Co-inhibitory receptors, Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Synonym	Signal-Regulatory Protein Alpha, SHPS1, CD172Antigen-Like Family Member A, Inhibitory Receptor SHPS-1, Macrophage FusionReceptor, PTPNS1, SIRP, P84, BIT, MFR, Brain-Immunoglobulin-Like Molecule With Tyrosine-BasedActivation Motifs, Brain Ig-Like Molecule With Tyrosine-Based ActivationMotifs, Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1, Tyrosine-ProteinPhosphatase Non-Receptor Type Substrate 1, Tyrosine Phosphatase SHP Substrate1, Signal-Regulatory Protein Alpha-1 , ignal-Regulatory Protein Alpha-2, Signal-RegulatoryProtein Alpha-3, SHP Substrate 1, CD172a Antigen, Myd-1 Antigen, MyD-1 Antigen,Sirp-Alpha-1, Sirp-Alpha-2, Sirp-Alpha-3, CD172A, SHPS-1, MYD-1, MYD1, Tyrosine-proteinphosphatase non-receptor type substrate 1.
Background	Signal-Regulatory Protein Alpha, SIRPA belongs to thesignal-regulatory-protein (SIRP) family, as well as the immunoglobulin super family. The membersof the SIRP family are receptor-typetransmembrane glycoproteins which are involved in the negative regulation of receptor tyrosinekinase-coupled signaling processes. SIRPA can be phosphorylatedby tyrosine kinases. The phospho-tyrosine residues of this PTP have been shownto recruit SH2 domaincontaining tyrosine phosphatases (PTP), and perform as substrates of PTPs. SIRPA take part in signal transduction mediated by a varietyof growth factor receptors. CD47 has been shown to be a ligand for SIRPA.
Description	SIRPA Human Recombinant expressed in E.Coli is a single,non-glycosylated polypeptide chain containing 370a.a. (27-373 a.a) andhaving a molecular weight of 40.4kDa.SIRPA is fused to a 23a.a. His-tag at N-terminusand purified by unique purification methods.
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSH MGSGVAGEEE LQVIQPDKSV LVAAGETATL RCTATSLIPV GPIQWFRGAGPGRELIYNQK EGHFPRVTTV SDLTKRNNMD FSIRIGNITP ADAGTYYCVK FRKGSPDDVE FKSGAGTELSVRAKPSAPVV SGPAARATPQ HTVSFTCESH GFSPRDITLKWFKNGNELSD FQTNVDPVGE SVSYSIHSTA KVVLTREDVH SQVICEVAHVTLQGDPLRGT ANLSETIRVP PTLEVTQQPV RAENQVNVTC QVRKFYPQRL QLTWLENGNV SRTETASTVTENKDGTYNWM SWLLVNVSAH RDDVKLTCQV EHDGQPAVSK SHDLKVSAHP KEQGSNTAAENTGSNERNIY.
Purity	>90.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	SIRPA protein solution (0.5mg/ml) containing PBS buffer(pH 7.4), 10% glycerol and 1mM DTT.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.