Recombinant Human CD55 Protein

Collections: Car-nk targets proteins, Cluster of differentiation (cd) proteins, Featured cd protein molecules, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Synonym	CD55 Antigen, DAF, CD55 Molecule, CHAPLE, Cromer Blood Group Antigen, Complement Decay-Accelerating Factor, CROM.
Background	Decay-Accelerating Factor Isoform 1 or CD55, is a protein that attached to the cell membrane through glycophosphatidylinositol (GPI) anchor. CD55 regulates the complement system in the outer membrane. The protein is a receptor to many types of enteroviruses such as coxsackieviruses. CD55 is widely scattered amidst hematopoietic & non-hematopoietic cells. The CD55 has a crucial part in tumorigenesis, autocrine loops for cell rescue and evasion of apoptosis, cell motility, invasiveness etc.
Description	CD55 expressed in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (35-353 a.a.) and fused to a 9 a.a. His Tag at C-terminus containing a total of 328a.a. and having a molecular weight of 36kDa. CD55 shows multiple bands between 40-57kDa on SDS-PAGE, reducing conditions and purified by unique purification methods.
Source	Sf9
AA Sequence	ADPDCGLPPD VPNAQPALEG RTSFPEDTVI TYKCEESFVK IPGEKDSVIC LKGSQWSDIE EFCNRSCEVP TRLNSASLKQ PYITQNYFPV GTVVEYECRP GYRREPSLSP KLTCLQNLKW STAVEFCKKK SCPNPGEIRN GQIDVPGGIL FGATISFSCN TGYKLFGSTS SFCLISGSSV QWSDPLPECR EIYCPAPPQI DNGIIQGERD HYGYRQSVTY ACNKGFTMIG EHSIYCTVNN DEGEWSGPPP ECRGKSLTSK VPPTVQKPTT VNVPTTEVSP TSQKTTTKTT TPNAQATRST PVSRTTKHFH ETTPNKGSGT TSHHHHHH.
Purity	Greaterthan 95.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	CD55protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10%glycerol.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.