Recombinant Rat Vascular Cell Adhesion Protein 1 (VCAM1) Protein (His)

Name: Recombinant Rat Vascular Cell Adhesion Protein 1 (VCAM1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-03541P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Rattus norvegicus (Rat) Vcam1.

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Product Overview

Description	Recombinant Rat Vascular Cell Adhesion Protein 1 (VCAM1) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P29534
Target Symbol	VCAM1
Synonyms	Vcam1; Vcam-1; Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; CD antigen CD106
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	PEYKTLAQIGDSMLLTCSTTGCESPSFSWRTQIDSPLNGKVKTEGAKSVLTMDPVSFENEHSYLCTATCNSGKLERGIQVDIYSFPKDPEIQFSGPLEVGKPVMVKCLAPDVYPIDRLEIELFKGDRLMKKQDFVDEMAKKSLETKSLEVIFTPVIEDIEKALVCRAKLYIDQTDSIPKERETVRELQVYTSPKNTEISVHPSTRLHEGAAVTMTCASEGLPAPEIFWSKKLDNGVLQLLSGNATLTLIAMRMEDSGIYVCEGVNLVGRDKTEVELIVQEKPFTVDISPGSQVAAQVGDSVVLTCAAVGCDSPSFSWRTQTDSPLNGEVRDEGATSTLTLSPVGVEDEHSYLCTVTCQRRKLEKTIQVEVYSFPEDPEIEISGPLVHGRPVTVNCTVPNVYPFDHLEIELLKGETTLLNKFLREEIGTKSLETKSLEMTFIPTAEDTGKALVCLAKLHSSQMESEPKQRQSTQTLYVNVAPKEPTIWVSPSPVPEEGSPVNLTCSSDGFPTPKILWSRQLKNGELQPLSQNTTLSFMATKMEDSGIYVCEGINEAGISKKSVELIIQGSSKDIQLTVFPSKSVKEGDTVIISCTCGSVPEIWIILKKKAKTGDMVLKSVNGSYTIRKAQLQDAGVYECESKTEVGSQLRSLTLDVKGKENNKDYFSP
Expression Range	31-697aa
Protein Length	Partial
Mol. Weight	77.4kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Database References	KEGG: rno:25361 STRING: 10116.ENSRNOP00000019377 UniGene: PMID: 29789783 Panax notoginseng saponins and Ginsenoside protect aorta form atherosclerosis via Rb1 via activation of Nrf2 and suppression of p38 - VCAM-1 signal pathway. PMID: 27620662 Berberine exhibited renoprotective effects in diabetic nephropathy (DN), and the underlying molecular mechanisms may be associated with changes in the levels and regulation of beta-arrestin 1/2 expression, as well as intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) levels in the kidney. PMID: 26531813 The combination of DC vaccination and antiangionesis inhibited the rats with malignant glioma by stimulating immune response, supressing the expression of angiogenesis-related proteins VEGF, VCAM, and ICAM-1 PMID: 27072237 VCAM1 was strikingly increased in neurons after intracerebral hemorrhage. PMID: 25868755 Activation of Wnt/beta-catenin signaling in AECs attenuated intercellular adhesion molecule 1 (ICAM-1)/vascular cell adhesion molecule 1 (VCAM-1)-mediated adhesion of both macrophages and neutrophils to AECs. PMID: 26351298 Adjuvant-induced arthritis was not associated with a change in VCAM1 levels. PMID: 25708190 Expression of E-selectin and CAMs was also modified in an age- and ischemia/reperfusion-dependent manner. PMID: 25182537 Upregulation of the adhesion molecules VCAM-1, ICAM-1, and E-selectin. PMID: 24289084 It is reasonable to conclude that PDGFBB significantly enhanced the expression of VCAM-1 in BM-MSCs, which facilitated the migration of BM-MSCs towards PDGFBB. PMID: 24100802 These findings suggested that glioma-induced change in VCAM-1 expression of bone marrow stem cells may play an important role in their tropism towards glioma. PMID: 22562815 Pretreatment of LPS-stimulated vascular endothelial cells with berberine dose-dependently decreased the number of adhered leukocytes and VCAM-1 expression at both RNA and protein levels. PMID: 23058024 results indicate that endothelin-1 stimulates arterial VCAM-1 expression through p38MAPK-dependent activation of neutral sphingomyelinases PMID: 22627111 Clerodendron glandulosum.Coleb leaf extract attenuates in vitro macrophage differentiation and expression of VCAM-1 and P-selectin in thoracic aorta of atherogenic diet fed rats. PMID: 21961520 A cholesterol diet does not play a role in the expression f VCAM-1 in the rat aorta. PMID: 22351665 VCAM1 is expressed at different levels in the various organs of rats after anaphylactic shock. The change of VCAM-1 is the most obvious in lungs. PMID: 21604443 Report specific adhesion and accumulation of VCAM-1-targeted ultrasound microbubbles to inflammatory endothelial cells under hemodynamic shear flow simulation. PMID: 22195873 black tea polyphenol theaflavin suppresses LPS-induced ICAM-1 and VCAM-1 expressions through blockage of NF-kappaB and JNK activation in intestinal epithelial cells PMID: 21184129 The expression of ICAM-1 and VCAM-1 was significantly decreased in the kidney of spontaneously hypertensive rats treated with Cordceps Sinensis. PMID: 20197615 Tanshinone IIA treatment leads to downregulation of ICAM-1 and VCAM-1 protein expression in brain microvascular endothelial cells. PMID: 20687137 Isoflurane, sevoflurane and desflurane down-regulated the expression of VCAM-1 in a dose-dependent manner. PMID: 20936719 Data indicates single-walled carbon nanotube exposure induces oxidative stress, thereby altering ICAM-1 and VCAM-1 expression. PMID: 21121367 Puerarin inhibits the expression of VCAM and P-selectin in the aorta of diabetic rats. PMID: 18095572 Fenofibrate can effectively prevent atherosclerosis by restoring NO concentration and down-regulating VCAM-1 expression. PMID: 18159007 Gene expressions of LOX-1 and VCAM-1 were higher in the aortic arch of HIV-1transgenic rats (Tg); the level of sICAM-1 was elevated in the HIV-1Tg rats but the ICAM-1 gene expression profile did not show any differences. PMID: 19997643 VCAM-1(+) immunoreactivity indicate an important role of choroid plexus in the induction of acute experimental autoimmune encephalomyelitis PMID: 19922364 Integrin alpha2beta1 on rat myeloma cells modulates interaction of alpha4beta1 integrin with vascular cell adhesion molecule-1 but not fibronectin. PMID: 12160302 During experimental allergic encephalomyelitis, a strong activation of spinal perivascular macrophages is observed accompanied by a consistently increased expression of VCAM-1. PMID: 12196270 iNOS and VCAM-1 are induced by angiotensin II in a pathway that involves p38 MAPK PMID: 15001568 Results are consistent with a role for the integrin alpha4beta1--vascular cell adhesion molecule-1 interaction in extension of sympathetic axons into the myocardium. PMID: 15366013 Suppression of the expression levels of VCAM-1 is associated with the amelioration of chronic rejection by various drugs, suggesting that these molecules are important key molecules in chronic rejection. PMID: 15919506 clusters of VCAM-1(+) immunoreactive cells localized in the choroid plexus epithelium and hypothalamo-hypophyses portal vessel system indicate an inflammation in acute autoimmune encephalitis PMID: 17064783 These results suggest that P2Y2R activation in salivary gland cells increases the EGFR-dependent expression of VCAM-1 and the binding of lymphocytes. PMID: 17599409 candidate genes for salt-sensitive hypertension in the SHRSP, including Edg1 and Vcam1, which are differentially expressed and lie on common and functionally important pathways. PMID: 17938382 Thromboxane receptor upregulates IL-1beta-induced vascular cell adhesion molecule 1 (VCAM-1) expression by enhancing the activation of c-Jun N-terminal kinase (JNK) pathway PMID: 18032781 the expression of VCAM-1 protein was upregulated in cerebral and common carotid arteries of simulated microgravity rats, and the upregulation of VCAM-1 may contribute to impaired endothelium-dependent relaxation in simulated microgravity rat vasculature PMID: 18221587 VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery. PMID: 19958991

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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