Recombinant Rat Plasminogen Activator Inhibitor 1 (SERPINE1) Protein (His-SUMO)

Name: Recombinant Rat Plasminogen Activator Inhibitor 1 (SERPINE1) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-04615P
Price: 549.6 USD
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: Antibody / cell therapy targets, Car-t cell therapy targets, Recombinant proteins

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Product Overview

Description	Recombinant Rat Plasminogen Activator Inhibitor 1 (SERPINE1) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P20961
Target Symbol	SERPINE1
Synonyms	Serpine1; Pai1; Planh1; Plasminogen activator inhibitor 1; PAI; PAI-1; Endothelial plasminogen activator inhibitor; Serpin E1
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	SPLPESHTAQQATNFGVKVFQHVVQASKDRNVVFSPYGVSSVLAMLQLTTAGKTRQQIQDAMGFNISERGTAPALRKLSKELMGSWNKNEISTADAIFVQRDLELVQGFMPHFFKLFRTTVKQVDFSEVERARFIINDWVERHTKGMISDLLAKGAVNELTRLVLVNALYFNGQWKTPFLEASTHQRLFHKSDGSTISVPMMAQNNKFNYTEFTTPDGHEYDILELPYHGETLSMFIAAPFEKDVPLSAITNILDAELIRQWKSNMTRLPRLLILPKFSLETEVDLRGPLEKLGMTDIFSSTQADFTSLSDQEQLSVAQALQKVKIEVNESGTVASSSTAILVSARMAPTEMVLDRSFLFVVRHNPTETILFMGQLMEP
Expression Range	24-402aa
Protein Length	Full Length of Mature Protein
Mol. Weight	58.6kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis.
Subcellular Location	Secreted.
Protein Families	Serpin family
Database References	KEGG: rno:24617 STRING: 10116.ENSRNOP00000001916 UniGene: Rn.29367

Gene Functions References

PAI-1 induces alveolar type II cell senescence through activating p53-p21-Rb pathway in fibrotic lung disease. PMID: 28722352
PAI-1 is implicated in the pathophysiology of depression. PMID: 27456456
Our results demonstrate the antifibrotic effect of PTX on radiation-induced lung fibrosis and its effect on modulation of PKA and PAI-1 expression as possible antifibrotic mechanisms. PMID: 28337441
Paclitaxel at low non-cytotoxic doses ameliorates renal fibrosis by inhibiting multiple steps in the TGF-beta1-induced PAI-1 signaling including Smads and mitogen-activated protein kinases. PMID: 26869361
Aldosterone at different concentrations exerts synergistic effect with TGF-beta1 to increase PAI-1 expression in hepatic stellate cells PMID: 26617694
inhibition of ERK1/2 activation has no therapeutic effect on kidney fibrosis in SNx possibly due to increased compensatory activation of the p38 and JNK signalling pathways with subsequent upregulation of PAI-1. PMID: 26415098
These results suggest the critical physiological role of TRL4-Fyn interaction in the modulation of PAI-1-tPA axis in astrocytes during neuroinflammatory responses such as ischemia/reperfusion injuries. PMID: 25106729
these results suggested that TGF-beta1 and Serpine 1 overexpression might play an important role in DVT formation and have predictive values. PMID: 26535698
PAI-1 contributes to the regulation of the inflammatory response in alveolar macrophages. PMID: 25342286
Neurite extension effects of valproic acid are mediated by modulating the tPA/plasminogen activator inhibitor (PAI)-1 system. PMID: 23378038
Together, because CPAI blocks both proteolytic and nonproteolytic tPA neurotoxicity, it is a promising therapeutics of neonatal HI injury either with or without infection. PMID: 22556277
PAI-1 promotes the proliferation, transforming into myofibroblasts, collagen synthesis, and inhibits apoptosis of pulmonary fibroblasts by activating Ca(2+), ERK and AKT signaling pathway. PMID: 23021499
adhesion scores, PAI-1 activity in peritoneal lavage fluid, and PAI-1 mRNA levels in peritoneal mesothelium were significantly greater in the open surgery group than the control and laparoscopic surgery groups PMID: 23218127
This study supports that a preceding progesterone-enriched milieu prevented the enhanced prothrombotic risk induced by a fructose-rich diet-elicited high PAI-1 production. PMID: 23016136
down-regulation of PAI-1 and MMP-9 might contribute to abnormal programming of renal growth in rats exposed to postnatal early overnutrition PMID: 21752621
The data demonstrate that PAI-1 siRNA inhibits alveolitis and pulmonary fibrosis in bleomycin-treated rats via inhibiting the proliferation and promoting the apoptosis of fibroblasts. PMID: 22659625
mRNA, protein and activity of matrix metalloproteinase (MMP)-2 and MMP-9 and mRNA of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor 1 (PAI-1) were quantified in abdominal aorta (AA) and femoral artery (FA). PMID: 22071631
Hydrocortisone up-regulates PAI-1 expression in a tissue-specific manner along with down-regulation of tPA activity in both normal and inflammatory conditions. PMID: 21412817
The activity of tissue plasminogen activator peaks transiently and then decreases in cortex and striatum along with delayed induction of PAI-1 in the inflammatory stage after reperfusion injury. PMID: 21193004
Data suggest PAI1 plays key role in modulating chromaffin cell neurosecretory function. Evidence is presented that in chromaffin cells, PAI1 is targeted to storage vesicles and co-released with catecholamine upon secretagogue stimulation. PMID: 21596853
PAI-1 derived from the neurovascular unit and peripheral vascular system participates as a positive regulator of the blood-brain barrier in facilitating the barrier function of the endothelial tight junctions. PMID: 21036181
Erigeron breviscapus injection can inhibit the expression of TNF-alpha and PAI-1, and increase the expression of tPA in rats with acute myocardial infarction. PMID: 21355198
PAI-1 may play a protective role against cardiovascular disease in diabetes PMID: 20538356
Expression of u-PA/PAI-1 mRNA and protein increased during the early stage of hyperoxic exposure and decreased during the middle and late stage. PMID: 19099788
The results suggest that SHP inhibits PAI-1 expression in VSMCs through the suppression of TGF-beta/Smad3 and AP-1 activity. PMID: 19887897
High glucose decreased the expressions of tPA and uPA, and increased PAI1. Losartan could partly reverse partly this expression. PMID: 17953365
Dahuang Zhechong Pill can down-regulate the expressions of TIMP-1 and PAI-1 mRNAs in renal tissues of rats with focal segmental glomerulosclerosis. PMID: 18471418
Inactivation of PAI-1 speeds lysis of a platelet-rich clot in rat mesenteric arterioles. Active PAI-1 participates in arteriolar microcirculation resistance to thrombolysis and its inactivation may shorten ischemic periods after microvascular obstruction. PMID: 11776323
The hexosamine pathway regulates the plasminogen activator inhibitor-1 gene promoter and Sp1 transcriptional activation through protein kinase C-betaI and -delta. PMID: 12105191
pathways of regulation of PAI1 induction and secretion by growth factors bFGF and PDGF in smooth muscle cells PMID: 12192306
Data show that recovery from obstruction-induced renal fibrosis was correlated with diminished expression of TGF-beta, collagen IV, and plasminogen activator inhibitor-1. PMID: 12365557
HIF1a and hypoxia response elements mediate the induction of PAI-1 gene expression by insulin via the PI3K/PKB pathway in primary rat hepatocytes. PMID: 12393531
possible role as the neurotrophic factor PMID: 12491782
Expression of functionally active PN-1 has been examined in vitro in rat aortic smooth muscle cells and in vivo in rat arterial media and its regulation in hypertensive rats. PMID: 12524238
Northern analysis confirmed upregulation of PAI-1, P2X4, and P15INK4B in cultured alveolar epithelial type I cells (AT1); in whole lung, all three proteins were detected in alveolar epithelium in a location consistent with expression in AT1 cells PMID: 12600825
hypoxia-dependent plasminogen activator inhibitor 1 expression is regulated by MAP kinases PMID: 12669121
In cells expressing RacG12V, ROS production was increased and PAI-1 mRNA levels as well as HIF-alpha nuclear presence were reduced under normoxia and hypoxia; whereas expression of RacT17N resulted in decreased ROS production and opposite effects. PMID: 12719791
Enhanced expression of clock genes may increase PAI-1 expression in concert with activated renin-angiotensin system in SHR heart PMID: 12777947
after transient focal cerebral ischemia, there are time dependent changes in PAI-1 expression PMID: 14512838
cellular carbonyl stress may stimulate PAI-1 synthesis in and release from adipose tissues through ROS formation PMID: 14610081
Pai1 expression is required for epithelial cell migration in in vitro wound healing. PMID: 15174100
PAI-1 and TGF-beta 1 in carotid glomus and autonomic ganglia were significantly increased (P < 0.01) in hypertensive rats PMID: 15201552
Altered expression of Pai-1 in diabetic nephropathy was associated with mesangial expansion. PMID: 15322501
Regression of glomerulosclerosis was linked to decreased tissue inhibitor of metalloproteinase-1 and plasminogen activator inhibitor-1. PMID: 15728787
Cardiac PAI-1 gene expression regulation is mediated through c-Jun NH2-terminal kinase activation. PMID: 15808835
the essential requirement of MAPK/AP-1 activation for TGF-beta1-induced PAI-1 expression is unique to rat mesangial cells PMID: 16105028
A dual role for PAI-1 in epithelial cell wound healing, both as a soluble inhibitor of proteolysis and also as a matrix-bound regulator of cell migration, is proposed. PMID: 17071586
This study indicate that aldosterone can increase PAI-1 mRNA and protein expression by cultured mesangial cells alone, which is independent of aldosterone-induced increases in TGF-beta(1) expression and cellular reactive oxygen species. PMID: 17394767
Renin induces ERK1/2 activation. Renin-activated pathway triggers cell proliferation along with TGF-beta1 and plasminogen activator inhibitor-1 gene expression. PMID: 17396111
PAI-1 plays a role in the occurrence of liver failure after excessive hepatectomy via accelerated maturation of pro-uPA and fibrinolytic factors. PMID: 17655862

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.