Recombinant Rat Muscarinic Acetylcholine Receptor M1 (CHRM1) Protein (His)

Name: Recombinant Rat Muscarinic Acetylcholine Receptor M1 (CHRM1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-10055P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Description	Recombinant Rat Muscarinic Acetylcholine Receptor M1 (CHRM1) Protein (His) is produced by our Yeast expression system. This is a cytoplasmic protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P08482
Target Symbol	CHRM1
Synonyms	Chrm1; Chrm-1Muscarinic acetylcholine receptor M1
Species	Rattus norvegicus (Rat)
Expression System	Yeast
Tag	N-6His
Target Protein Sequence	RIYRETENRARELAALQGSETPGKGGGSSSSSERSQPGAEGSPESPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDSEAQAPTKQPPKSSPNTVKRPTKKGRDRGGKGQKPRGKEQLAKRKTFSLVKEKKAART
Expression Range	210-366aa
Protein Length	Cytoplasmic Domain
Mol. Weight	19.1kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Subcellular Location	Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Protein Families	G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM1 sub-subfamily
Database References	KEGG: rno:25229 STRING: 10116.ENSRNOP00000024785 UniGene: PMID: 29705702 Results establish an intracellular pathway linking M1 receptors, inositol triphosphate receptors, and ubiquitin proteasome system activity to object memory destabilization and suggest a previously unacknowledged role for cholinergic signaling in long-term memory modification and storage. PMID: 29030298 Study examined the expression of M1 muscarinic ACh receptors in the hippocampus at 7 and 30 days following experimental bilateral vestibular lesions (BVL) in rats using autoradiography. Analysis of the brain tissue revealed a significant reduction in the number of M1 receptors throughout the hippocampus and striatum at 30 days but not at 7 days following BVL. PMID: 27569857 M1 and M3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product. PMID: 26626425 Our results suggest that the mechanism for the deficits in learning and memory of rats with chronic fluorosis may be associated with the decreased expressions of M1 and M3 in mAChRs PMID: 25417050 Neonatal hypoglycemia decreased total muscarinic receptors (p < 0.001) with reduced muscarinic M1, M2, and M3 receptor genes (p < 0.001) in one month old rats. PMID: 25474381 M1 cholinergic receptor activation in the central nervous system can modulate endotoxin tolerance in rat liver PMID: 25008070 Selective M1 and M4 receptor activation each improved memory. PMID: 24480931 Blockade of M1-cholinoreceptors does not prevent the inhibitory effect of carbacholine on contractility of the atrial and ventricular myocardium. PMID: 24288746 M1 mAChR may have a role (via the PKC-ERK1/2 pathway) in the neuroprotective effects of lesatropane PMID: 23988164 The results of this study findings suggested that M1R mechanisms in the BLa are complex, involving postsynaptic effects as well as regulating release of neurotransmitters from presynaptic terminals. PMID: 23559406 There is a significant decrease in muscarinic M1 receptors in the brain stem following spinal cord injury. PMID: 23184186 M1-mAChRs function at both surface and intracellular sites in telencephalon neurons including the hippocampus PMID: 23678982 Muscarinic receptor expression is developmentally regulated; M1, M3, and M4 receptors are the main subtypes expressed in oligodendrocyte progenitor cells. PMID: 21913336 Report therapeutic potential of the M(1) receptor positive allosteric modulator BQCA for the treatment of memory deficits observed in Alzheimer's disease. PMID: 23085025 Data suggest that Chrm1 is down-regulated in cell membranes of parotid glands in a model of periodontitis and participates in potassium release; Chrm1 expression appears to return to control level upon indomethacin treatment of periodontitis. PMID: 22513211 Muscarinic receptor activation determines the downstream effects of store-operated Ca(2+)-entry on neuronal membrane excitability and energy metabolism. PMID: 22088219 Expression of muscarinic receptor M1, M3, M5 subunits decreased in the flocculus after unilateral labyrinthectomy. PMID: 19160866 Use of scannning mutagenesis uncovers helix 8 of the M1 mAChR, and a G protein recognition site is delineated in the activated state of the receptor. PMID: 21247934 study showed that M and M receptors, IP3 and cGMP were functionally regulated during diabetes as function of age PMID: 21054404 These data indicate a novel mechanism of synaptic plasticity in which activation of M1 receptors results in AMPA receptor endocytosis, via a mechanism involving interactions between GluA2, GRIP and liprin-alpha. PMID: 19534762 Chrm1 receptor is located on presynaptic glutamatergic terminals afferent to the recorded pyramidal neuron, thus decreasing glutamate release. PMID: 20600670 muscarinic M(1) receptor showed a significant decrease and muscarinic M(3) receptor subtype showed a significant increased binding in the cerebral cortex of hypoglycemic rats compared to diabetic and control PMID: 21126518 Cholinergic activation through M(1)-like receptor enhances basket-cell activity & promotes the generation of nested theta & gamma rhythms, enhancing hippocampal function and associated performance. PMID: 20433901 Thus, the current study identifies ASIC1a as a novel target for muscarinic signaling. PMID: 19730136 Acetylcholine through muscarinic M1 and M3 receptors stimulated calcium release from the pancreatic islets. PMID: 19554469 The results indicate that muscarinic M(1) modulation of Ca(V)2.1 Ca(2+) channels in these neurons involves phosphoinositide hydrolysis. PMID: 19883739 Results highlight long-term low dose somatotropin and insulin treatment in regulating muscarinic M1 and M3 cholinergic and NMAR1 and mGluR5 glutamate receptor subtypes in aging rats and rejuvenation of brain function. PMID: 19666081 no change on muscarinicM1 mRNA expression was found in the hippocampus of isolation reared rats PMID: 12384226 In the neuromuscular junction, the modulation of Ach release via M1 and M2 muscarinic autoreceptors differs between the developing and adult rat PMID: 12534975 This represents the first demonstration of M1 receptors on neuronal progenitor cells and supports the notion that M1 muscarinic receptors may play a role in development of the nervous system PMID: 14675151 muscarinic acetylcholine receptor-stimulated inositol 1,4,5-trisphosphate production is augmented by synaptic activity to facilitate Ca2+ release in hippocampal neurons PMID: 15342646 central muscarinic M1 and M3 receptor subtypes functional balance is suggested to regulate sympathetic and parasympathetic activity, which in turn control the islet cell proliferation and glucose homeostasis PMID: 15350825 M1 mAChR stimulation leads to GSK-3beta inactivation. Cross-talk between muscarinic signaling and Wnt components underlie neuroprotective effect of M1 mAChR activation. PMID: 15474371 endogenous GRK2 is a key regulator of M1 mACh receptor signaling in hippocampal neurons PMID: 15743771 Stimulation of M1 muscarinic acetylcholine receptors activates endogenous transient receptor potential-canonical subtype 6 (TRPC6) channels in neuronal PC12D cells. PMID: 15994335 KCNQ channel currents in striatal medium spiny neurons were potently reduced by M1 muscarinic receptors. PMID: 16093396 ductules and epididymis suggests that these receptors play a role in the modulation of luminal fluid composition and smooth muscle contraction. PMID: 16160857 study shows that infection with low numbers of Hymenolepis diminuta leads to modulation of the cholinergic components of the myenteric plexus & M2 receptor mRNA & that large number of worms result in suppression in relative expression of M1 receptor mRNA PMID: 16541262 results show that the dicyclomine did not affect IA and CFC consolidation, suggesting specific involvement of M1 muscarinic receptor only in acquisition these tasks, and these effects was not state-dependent PMID: 16647280 Data suggest that acetylcholine acting on M1 and M2 acetylcholine receptors may contribute to selective excitability enhancement or depression in individual, rostrally projecting sensory neurons. PMID: 16820015 Ach agonists inhibit the non-quantal secretion of ACh from motor nerve terminals by acting on post-synaptic M1 receptors coupled to Ca(2+) channels in the sarcolemma to induce sarcoplasmic Ca(2+)-dependent synthesis and the release of NO. PMID: 17561934 The findings suggest that activation of M1-type muscarinic cholinergic receptors in the dorsomedial striatum facilitate the flexible shifting of response patterns by maintaining or learning a new choice pattern once selected. PMID: 17709264 Four derivatives (5a-5c and 5e) showed considerable M1 receptor binding affinity (in vitro) and elicited beneficial effects in vivo memory and learning models (Rodent memory evaluation, plus and Y maze studies). PMID: 17719699 Study suggests that formation of a direct hydrogen bond between the highly conserved side chains of Asp71 and Asn414 may be a critical feature stabilizing the activated state of the M1 muscarinic acetylcholine receptor. PMID: 17823120 Taken together these data suggest that the level of synaptic activity may determine the different kinases recruited to regulate M1 mACh receptor desensitization in neurons. PMID: 17908240 insulin modulates binding parameters and gene expression of total and muscarinic M1 receptors. PMID: 17960828 Data show that L- and N-type calcium channel current, but not M-current inhibition by M1 muscarinic receptors requires DAG lipase activity. PMID: 18247369 These results provide further evidence of the expression of the M(1) muscarinic receptor subtype in the rat heart PMID: 18443764 Data suggest that 77-LH-28-1 is a potent, selective, bioavailable and brain-penetrant agonist at the M1 mAChR. PMID: 18454168

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

📧 inquiry@betalifesci.com 📞 +1(201) 888-7983

Contact Us Now for Inquiries, Orders, and Questions

Recombinant Rat Muscarinic Acetylcholine Receptor M1 (CHRM1) Protein (His)

Recombinant Rat Muscarinic Acetylcholine Receptor M1 (CHRM1) Protein (His)

Product Overview

Target Details

FAQs