Recombinant Rat IFNGR1 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2559
Recombinant Rat IFNGR1 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2559
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | Fc |
Host Species | Rat |
Accession | Q6P6T3 |
Background | The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD119 (cluster of differentiation 119), also known as IFNGR1 ( interferon gamma receptor 1), is part of the heterodimeric gamma interferon receptor which consists of IFNGR1 (CD119) and IFNGR2. The IFNGR1 gene encodes the ligand-binding chain (alpha) of the interteron receptor while IFNGR gene encodes the non-ligand binding partner. The ability of the interferon-γ was achieved through binding to the interferon receptor CD119. After binding, the products of activated T-lymphocytes interferon-γ exerts antiviral activity, growth inhibitory effect, and several immune- regulatory activities on a variety of cell types. |
Description | A DNA sequence encoding the rat IFNGR (Q6P6T3) (Met1-Ser241) was expressed with the Fc region of human IgG1 at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Ala 17 |
AA Sequence | Met1-Ser241 |
Molecular Weight | The recombinant rat IFNGR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 466 a.a. and has a predicted molecular mass of 52.4 kDa. The apparent molecular mass of the protein is approximately 117 and 65-80 kDa in SDS-PAGE under reducing conditions. |
Purity | (22.7+76.3)% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured by its binding ability in a functional ELISA. Immobilized rat IFNGR-Fc at 10 ug/ml (100 ul/well) can bind biotinylated rat IFNG-Fc. The EC50 of biotinylated rat IFNG-Fc. The ED50 for this effect is typically 0.5-4 ng/mL in the presence of 10 ng/mL of recombinant rat IFN-gamma. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |