Recombinant Rat Histamine H1 Receptor (HRH1) Protein (His&Myc)

Name: Recombinant Rat Histamine H1 Receptor (HRH1) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-00151P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Rat Histamine H1 Receptor (HRH1) Protein (His&Myc) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Activity	Not tested.
Uniprotkb	P31390
Target Symbol	HRH1
Synonyms	(H1R)(HH1R)
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	VKIYKAVRRHCQHRQLTNGSLPSFSELKLRSDDTKEGAKKPGRESPWGVLKRPSRDPSVGLDQKSTSEDPKMTSPTVFSQEGERETRPCFRLDIMQKQSVAEGDVRGSKANDQALSQPKMDEQSLNTCRRISETSEDQTLVDQQSFSRTTDSDTSIEPGPGRVKSRSGSNSGLDYIKITWKRLRSHSRQYVSGLHLNRERKAAKQ
Expression Range	211-415aa
Protein Length	Partial
Mol. Weight	30.6 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.
Subcellular Location	Cell membrane; Multi-pass membrane protein.
Protein Families	G-protein coupled receptor 1 family
Database References	KEGG: rno:24448 STRING: 10116.ENSRNOP00000009775 UniGene: PMID: 28722302 findings indicate that histamine H1, H2, and H3 receptors are present in rat olfactory epithelium and may play a physiological role in olfactory transmission. PMID: 28964277 This study demonstrates that AITC-induced nociception in vivo depends on TRPA1 and is decreased by the blockade of the NK1 receptor for SP, the H1 receptor for histamine and the 5-HT1A and 3 receptors for 5-HT PMID: 27904941 An H1 receptor blocker did not affect exercise-induced improvement in learning and memory in rats, so these receptors may not play a role in this process. PMID: 25192644 Isolated rat mesenteric collecting lymphatics were treated with 1- to 100-muM histamine. Pharmacologic blockade of either H1 or H2 histamine receptors significantly inhibited the response to histamine. PMID: 24702851 Activation of H1R was required dopaminergic lineage during ventral midbrain development. PMID: 25112718 Na+-Ca2+ exchangers coupled to H1 receptors and hyperpolarization-activated cyclic nucleotide-gated channels linked to H2 receptors co-mediate the strong postsynaptic excitatory action of histamine on al vestibular nucleus neurons PMID: 23713466 These results show that histamine, by activating H(1)R, has a neurogenic effect in clonal conditions PMID: 23497494 Histamine within the nucleus tractus solitarii may play a role in regulating cardiovascular homeostasis via activation of H1 receptors. PMID: 21622829 at the level of the hippocampus, histamine through its H1 and H2 receptors, mediates orofacial region pain PMID: 21602597 H1 receptors play an excitatory role in the modulation of the discharge activities of the inspiratory neurons in neonatal rat brainstem slices. PMID: 20117984 Based on its ability to reverse amphetamine-induced PPI deficits, blockade of histamine H(1) receptors seems to contribute to the therapeutic effect of the antipsychotic clozapine. PMID: 20811878 Studies using various antagonists indicate hippocampal Hrh1 plays an important role in encoding, retention, and retrieval processes of spatial working memory. PMID: 20096284 Activation of H1-receptors in vasopressinergic neurons results in downstream activation of NO synthase, which then mediates the H1-receptor effects. PMID: 12419527 ThEA stimulation evoked amylase release via H1 receptor but was not followed by L-arginine/nitric oxide pathway activation. PMID: 12581497 H1, H2, and H3 histamine receptor mRNA detected in modiolus but not in lateral and medial portions of the cochlea. First evidence of H1, H2, and H3 histamine receptor mRNA in rat cochlea. PMID: 12634496 histamine H(1)-receptor and PKC are involved in the signal transduction pathway, responsible for histamine-stimulated NGF secretion from cultured astrocytes PMID: 15167971 In rat thalamus, histamine H(1) receptor and isoforms of H(3) receptor were expressed predominantly in the midline and intralaminar areas PMID: 15899242 Postischemic increase in H(1) receptor mRNA expression in the caudate-putamen. PMID: 16181737 data represent the first demonstration of H1 receptor expression in sympathetic preganglionic neurons and suggest that histamine acts to regulate excitability of these neurons via a direct postsynaptic effect on H1 receptors PMID: 16354729 Processing of proprioceptive information at the level of the mesencephalic trigeminal neurons is controlled via histamine H1 and H3 receptors through different postsynaptic mechanisms. PMID: 16797837 Results suggest that hippocampal H1 receptors play an important role in working memory processes on radial maze performance and the decrease in hippocampal theta power is associated with the working memory deficit induced by the blocking of H1 receptors. PMID: 17562388 Data show that the synergistic effect of histamine and IL-6 on NGF secretion from cultured astrocytes is evoked by histamine stimulation of IL-6 secretion via a H1-receptor-PKC-MAPK signalling pathway. PMID: 18345501 Data show that cetirizine, a histamine H1 receptor antagonist, decreases the first stage of orthodontic tooth movement in rats. PMID: 18345503 The expression of H1 receptor mRNA was up-regulated in the hypothalamus and brainstem, but not in the cerebral cortex after 2 G hypergravity load in rats. PMID: 18607976 These results suggest that chewing-induced histamine release in the hippocampus and the subsequent H1 receptor activation may be essential to rescue stress-suppressed synaptic plasticity. PMID: 19393269

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.