Recombinant Rat Endothelin-1 (EDN1) Protein (hFc)

Name: Recombinant Rat Endothelin-1 (EDN1) Protein (hFc)
Brand: Beta LifeScience
SKU: BLC-10950P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Rat Endothelin-1 (EDN1) Protein (hFc) is produced by our Yeast expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P22388
Target Symbol	EDN1
Synonyms	Edn1; Endothelin-1; ET-1; Preproendothelin-1; PPET1) [Cleaved into: Big endothelin-1]
Species	Rattus norvegicus (Rat)
Expression System	Yeast
Tag	C-hFc
Target Protein Sequence	CSCSSLMDKECVYFCHLDIIW
Expression Range	53-73aa
Protein Length	Full Length
Mol. Weight	29.1 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
Subcellular Location	Secreted.
Protein Families	Endothelin/sarafotoxin family
Database References	KEGG: rno:24323 STRING: 10116.ENSRNOP00000019361 UniGene: PMID: 28273940 liraglutide suppresses ET-1 and enhances eNOS/sGC/PKG pathways in treatment of monocrotaline-induced pulmonary arterial hypertension PMID: 27581840 In heart irradiation groups, 3-fold up-regulation of both ET-1 and COX-2 was observed. In pelvis irradiation groups, 3-fold up-regulation of ET-1 was seen, but not significant changes in COX-2 gene expression have observed at distant heart tissues after pelvis irradiation. PMID: 28508833 the mRNA expression of prepro-endothelin-1 (ppET-1), as well as that of the ETA and ETB receptors, were quantified by real-time PCR in these veins... exercise training mobilizes endothelin-1 (ET-1) to reinforce the Ang II-induced responses mainly through ETA activation PMID: 28012855 tonus of the remodeled spiral artery determined by the endovascular trophoblast response to the ET1 and NO systems PMID: 27697218 EDN-1 overexpressed bone marrow-derived mesenchymal stem cells showed increased proliferation and significantly increased osteogenesis potential ability than vector transfected control PMID: 28432001 Activation of renal medullary P2Y2/P2Y4 receptors promotes ET-1-dependent natriuresis in ovariectomized rats. PMID: 28468962 The results of this study indicated that ET1 signaling is critical in maintaining the excitability of glutamatergic neurons in the hippocampus and, thus, in modulating anxiety-like behaviors. PMID: 28302570 the increased contractile response via ET-1 receptors in the ophthalmic artery after 48 hours may elicit negative retinal consequences due to a second ischemic period. PMID: 27322388 ET-1-ROCK interactions contribute to decreased alveolar and vascular growth. PMID: 27760762 Nur77 decreases ET-1 expression by suppressing NF-kappaB and p38 MAPK. PMID: 27765761 Fermented red ginseng ameliorated endothelial dysfunction by downregulation of endothelin-1 (ET-1) and adhesion molecules in the aorta. PMID: 27322312 ET system contributes to pathological neovascularization in diabetes as evidenced by restoration of functional angiogenesis by bosentan treatment and prevention of linagliptin-mediated improvement of angiogenesis in the in vitro model PMID: 26631506 Linagliptin lowered plasma ET-1 levels in diabetes, and reduced ET-1-induced vascular contraction. TLR2 antagonism in diabetic basilar arteries reduced ET-1-mediated cerebrovascular dysfunction and improved endothelium-dependent vasorelaxation PMID: 26898123 Lipopolysaccharides significantly promoted ET-1-induced pulmonary arterial smooth muscle cells proliferation. PMID: 27470334 ET-1-mediated elevation of intracellular Ca(2+) is strongly linked to renal microvascular contraction and is crucial for ET-1-induced contraction of SMC PMID: 26682937 ET-1 may be one of the upstream effectors for programmed neuronal necrosis through abnormal LIMK2 over-expression by ROCK1. PMID: 26438559 Our data demonstrates that endothelin-1-mediated influx of extracellular Ca(2+) activates transient receptor potential canonical channels 1 and 6 in cerebral vascular smooth muscle cells. PMID: 25939574 cerebral microvasculature is relatively insensitive to ET-1-induced functional down-regulation PMID: 25014910 Study showed that store-operated ion channels Orai1 and TRPC3 contribute to endothelin/bradykinin effects in vitro and in vivo PMID: 25873305 hypoxia and anoxia via activation of endothelin-1 at the critical window of heart development inhibits cardiomyocyte proliferation and decreases myocyte endowment in the developing heart PMID: 25692855 in the myocardium of old rats the gene expression of endothelin-1, iNOS and TNF-alpha was increased, and the gene expression of endothelin B receptors and eNOS was reduced, compared with young rats PMID: 25446983 ET-1 and ETAR are potential targets responsible for the observed synergism effect in the hypertensive atherosclerotic process through enhancement of ET-1 binding, ET-1 binding, ETAR expression, and ET-1-induced mitogenic actions in aortic VSMCs PMID: 25824048 ET-1 stimulates ETA -mediated NADPH oxidase-dependent ROS generation, which inhibits endothelial NO bioavailability and contributes to ET-1-induced contraction in healthy penile arteries. PMID: 25091502 ROCK-2 inhibition restores ET-1-mediated NO production after the LPS pretreatment, in part, through an increase in actin depolymerization PMID: 25243430 Salt or water loading increased ET-1 mRNA in inner medullary collecting duct PMID: 25587122 Data show an inappropriate secretion of endothelin 1 (ET-1) in obese animals with a parallel DNA damage in their lungs. PMID: 25517973 High glucose induced ET-1 production that mediated the EMT induced by high glucose in renal tubular epithelial cells, and HIF-1alpha acted as the upstream signal to regulate ET-1. PMID: 24447812 We conclude that enhanced ET-1 signaling is part of a pathologic mechanism associated with adverse cerebrovascular outcomes of obstructive sleep apnea ( PMID: 25425077 Restriction in cerebral blood flow was observed with upregulation of endothelin-1 in cerebral ischemia. PMID: 25228257 These findings identify a novel mechanistic interaction between the ET-1/ETB receptor axis and CX3CL1/CX3CR1 in mediating pulmonary angiogenesis and vascular monocyte accumulation in experimental HPS. PMID: 24731444 The combination therapy prevented pulmonary artery injury and angiogenesis of the arteries by reducing the level of ET-1 and promoting the level of 6-keto-PGF1alpha in the blood PMID: 24854661 Endothelin-1 and endothelin-2 initiate and maintain contractile responses by different mechanisms in rat mesenteric and cerebral arteries. PMID: 23941276 Ang II enhances ET-1-induced vasoconstriction by upregulating ETAR expression and ET-1/ETAR binding, which may be because of the AngII/Ang II receptor pathways and the activation of PKC or ERK. PMID: 25088996 Altered pulmonary vascular reactivity in hypertension may be related to a loss of endothelial buffering of vasoconstriction and decreased leptin-induced vasodilation in conditions of increased endothelin-1. PMID: 24499246 The insulin/endothelin-1 vasoconstrictor pathway is more active in GFA than in SFA. PMID: 23995100 These results indicated that apocynin alleviated CIH-induced hypertension by inhibiting NADPH oxidase, further leading to the reduced vasoconstrictor ET-1 level and oxidative stress. PMID: 23592126 COX-2 and NFATc3 signaling are involved in ET-1-induced hypertrophy of neonatal rat cardiomyocytes. PMID: 24291639 These findings support the hypothesis that vascular endoplasmic reticulum stress-mediated activation of ET-1 may be an underlying cause of impaired vasomotor responsiveness to insulin and endothelial dysfunction. PMID: 24304569 ET-1 induces a glycolytic switch in pulmonary arterial endothelial cells via the redistribution of uncoupled eNOS. PMID: 24392990 Endothelin-1 has a role in activating ERK1/2 via transactivation of platelet-derived growth factor receptor in rat L6 myoblasts PMID: 24735959 Levels of ET-1 peptide increase in kidney tissues after lipopolysaccharide (LPS) administration time-dependently with a rise in plasma ET-1 concentration, compared to control rats. PMID: 24641950 ET-1-induced cardiomyocyte hypertrophy is mediated through peptidylprolyl cis/trans isomerase (Pin1) activation. PMID: 24657892 Status epilepticus induces vasogenic edema via TNF-alpha/endothelin-1-mediated two different pathways. PMID: 24040253 CIH induced increased ET-1. PMID: 23662699 NO, PGI2 and ET-1 expression are regulated by 17beta-estradiol in pulmonary hypertension PMID: 22936708 ET-1 enhances the phosphorylation of epidermal growth factor receptor (EGFR) in cultured vascular smooth muscle cells. PMID: 23537435 The increased responsiveness to ET-1 induced by intermittent hypoxia in pulmonary arteries of rats was due to increased expression of ETA receptors PMID: 23555567 Epididymal fat and soleus muscles from rat were incubated with ADM at concentration of 100 nM for the study of the gene expression and secretion of tumour necrosis factor (TNF-alpha), EDN-1, leptin, adiponectin, interleukin 1beta (IL-1beta), and IL-6. PMID: 22956308 In conclusion, the cardioprotective effects of EGb761 on markers of ADR-induced acute cardiotoxicity appeared to have been mediated by the regulation of inflammatory and vasoactive mediators, as well as the inhibition of membrane lipid peroxidation. PMID: 23898483

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Rat Endothelin-1 (EDN1) Protein (hFc)

Recombinant Rat Endothelin-1 (EDN1) Protein (hFc)

Product Overview

Target Details

FAQs