Recombinant Rat Cytochrome P450 1A1 (CYP1A1) Protein (His&Myc)

Name: Recombinant Rat Cytochrome P450 1A1 (CYP1A1) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-00172P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Rat Cytochrome P450 1A1 (CYP1A1) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Activity	Not tested.
Uniprotkb	P00185
Target Symbol	CYP1A1
Synonyms	(CYP1A1)(CYPIA1)(Cytochrome P450 form 6)(Cytochrome P450-C)(Cytochrome P450-P1)(Cytochrome P450MT2)(Hydroperoxy icosatetraenoate dehydratase)
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	PSVYGFPAFTSATELLLAVTTFCLGFWVVRVTRTWVPKGLKSPPGPWGLPFIGHVLTLGKNPHLSLTKLSQQYGDVLQIRIGSTPVVVLSGLNTIKQALVKQGDDFKGRPDLYSFTLIANGQSMTFNPDSGPLWAARRRLAQNALKSFSIASDPTLASSCYLEEHVSKEAEYLISKFQKLMAEVGHFDPFKYLVVSVANVICAICFGRRYDHDDQELLSIVNLSNEFGEVTGSGYPADFIPILRYLPNSSLDAFKDLNKKFYSFMKKLIKEHYRTFEKGHIRDITDSLIEHCQDRRLDENANVQLSDDKVITIVFDLFGAGFDTITTAISWSLMYLVTNPRIQRKIQEELDTVIGRDRQPRLSDRPQLPYLEAFILETFRHSSFVPFTIPHSTIRDTSLNGFYIPKGHCVFVNQWQVNHDQELWGDPNEFRPERFLTSSGTLDKHLSEKVILFGLGKRKCIGETIGRLEVFLFLAILLQQMEFNVSPGEKVDMTPAYGLTLKHARCEHFQVQMRSSGPQHLQA
Expression Range	2-524aa
Protein Length	Full Length of Mature Protein
Mol. Weight	66.7 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15alpha and C16alpha positions. Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system. Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer. May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent).
Subcellular Location	[Cytochrome P450 1A1]: Cytoplasm.; [Cytochrome P450MT2A]: Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.; [Cytochrome P450MT2B]: Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Protein Families	Cytochrome P450 family
Database References	KEGG: rno:24296 STRING: 10116.ENSRNOP00000026473 UniGene: PMID: 28879796 CYP1A1 and CYP1B1 mRNA expression levels were significantly reduced from day 7 and 10, respectively. These results suggested that CpG island hypermethylation of the CYP1A1 and CYP1B1 promoters regulate the low expression of genes involved in the occurrence of isoniazidinduced liver injury PMID: 29115507 We showed that the enhanced activity of the CYP1A1 mutant towards TCDD was due to more efficient binding of the substrate in the active site even though the mutated site was over 2.5nm away from the catalytic center PMID: 28780123 Data suggest that two inhibitors from grapefruit juice (naringenin and 6',7'-dihydroxybergamottin) block oxidation of 7-ethoxyresorufin via competitive binding to the active site of both human and rat CYP1A1. PMID: 27444380 Hexachloronaphthalene was found to be a strong inducer of maternal and fetal CYP1A1 in liver and placenta. PMID: 26403959 drugs acting as D2-dopamine receptor antagonists can modify several hormone systems that regulate the expression of CYP1A1, CYP1A2 and CYP1B1, and may affect the toxicity and carcinogenicity outcome of numerous toxicants and pre-carcinogenic substances PMID: 26466350 Incense smoke exposure significantly induced the expression of CYP1A1, CYP1A2, and CYP1B1 mRNAs in both lung and liver tissues. PMID: 24557852 Differential expression of CYP1A1 and CYP1A2 genes in H4IIE rat hepatoma cells exposed to TCDD and PAHs PMID: 25555259 BP-lowering effect of sEH inhibition in I3C-induced Cyp1a1-Ren-2 transgenic rats. PMID: 25224811 The influence of dietary lipids on the ontogeny of XMEs was also evaluated. mRNA and protein levels of phase I (CYP1A1, CYP1A2, and CYP1B1) and phase II (NAD(P)H:quinone acceptor oxidoreductase 1 and GSTP1) enzymes were analyzed. PMID: 25164943 Hypertension fails to disrupt white matter integrity in young or aged Fisher (F44) Cyp1a1Ren2 transgenic rats. PMID: 25407269 5-fold reducing of vitamin content in rat diet lead to significant changes in activity and inducibility of cytochrome P450 of CYP1A and 3A family, which play a key role in the detoxification and metabolism of drugs. PMID: 25300103 High-altitude hypoxia decreases the activity and expression of CYP1A1 and NAT2, but increases that of CYP2D6. PMID: 24557547 This study aimed to assess cerebrovascular alterations in response to different durations (4 or 6 months) of controlled hypertension in an inducible transgenic rat model of hypertension (Cyp1a1-Ren2) as compared with normotensive litter mate controls. PMID: 24119481 Increase of CYP1A1 mRNA level may not only reduce the carcinogenicity of foreign compounds, but may also activate some cardinogens.In case of transporters, an increase of their expression in the body may lead to increased fetoprotection. PMID: 25011215 CYP1A1 expression in liver and ovaries is increased following chemical induction, along with microRNAs. PMID: 24727239 A metabolite, derived from the action of CYP1A1 and a nitroreduction-reaction process, has a key role in the bioactivation of niclosamide. PMID: 23956140 The results suggest that biotin does not influence the CYP1A-mediated metabolism of xenobiotics. PMID: 23984390 role of bilirubin, tocopherol and corticosterone as mediators of cold induction of CYP1A in the liver was evaluated; results indicate that these compounds can be involved in cold induction of CYP1A, but none of them is the only mediator in this process PMID: 23658888 Severe liver cirrhosis markedly reduces AhR-mediated induction of CYP1A1 in rats by decreasing the transcription of target genes. PMID: 23626760 The expression of genes involved in action potential generation and Ca(2+) homeostasis of cardiomyocytes in hypertensive cyp1a1ren-2 transgenic rats, was investigated. PMID: 23060473 CYP1A2 enzyme activity was much more markedly increased in Sprague-Dawley than Wistar rats and CYP1A1 activity was induced to similar levels. PMID: 22785257 In the liver, cabbage and sauerkraut juices decreased the activities of CYP1A1 and CYP1A2. PMID: 22173777 Organic pollutants from river water could induce the activity of the CYP1A1 gene promoter and induce the expression of CYP1A1 mRNA in H4IIE cells. PMID: 19069647 As(III) decreases CYP1A1, CYP1A2, CYP3A23, and CYP3A2 expression in freshly isolated rat primary hepatocytes. PMID: 22159698 Data from a model applying fresh smoke (or the vapor phase/particulate matter) to lung slices in 3-day culture confirm up-regulation of stress-response genes (CYP1A1, IL-1beta, and bradykinin B1 receptor) along with damage to alveolar structure. PMID: 22266391 Role of cytochromes P450 in metabolism of carcinogenic aristolochic acid I. PMID: 22167220 Molecular mechanisms of cold-induced CYP1A activation in rat liver microsomes PMID: 21505853 Rat Ahr and Cyp1a1 reached highest expression during gestation days 15 and 18, which might indicate different response to Ahr ligands in placental Cyp1a1 induction during rat gestation. PMID: 21666223 These results suggest that maternal consumption of either red raspberry leaf or some of its constituents leads to long-term alterations of CYP activity in female offspring. PMID: 21115944 Data show that only rLSEC-PEM-Hepatocyte cultures exhibited increasing CYP1A1/2 and CYP3A activity. PMID: 21103392 Amiloride reduces arterial pressure in cyp1a1ren-2 transgenic rats in association with increased renal sodium excretion without affecting renal vascular function PMID: 20683339 These studies demonstrated that Danshen aqueous extract affected the metabolism of CYP1A2 substrates through competitive inhibition and altered their clearance. PMID: 20663043 Metabolism of polychlorinated dibenzo-p-dioxins (PCDDs) by recombinant yeast cells expressing either rat CYP1A1 or CYP1A2 was examined. PMID: 12054491 Enterocytic CYP1A (CYP1A1) showed a sharp increase at weaning, plateauing at adult levels by 60 days PMID: 12162855 CYP1A1 is repressed in rat keratinocytes because of the interactions of two negative regulatory elements PMID: 12200118 Differentiation-dependent induction of CYP1A1 in cultured rat small intestinal epithelial cells, colonocytes, and human colon carcinoma cells: basement membrane-mediated apoptosis PMID: 12210751 Cyp1a1 is upregulated after treatment with Aroclor in rat liver PMID: 12396271 analysis of metabolic pathways involving CYP1A1 and dioxins, and comparison to the human homolog PMID: 12464257 Hepatic upregulation of the aryl hydrocarbon receptor may modulate the potential for benzo(a)pyrene toxicity via the activation of cytochrome P450 and subsequent deposition of lipophillic metabolites to developing cerebral cortex and hippocampus. PMID: 12927582 Together, these results demonstrate a novel mechanism of CYP1A1 transcriptional repression that does not involve any previously reported negative regulatory elements for CYP1A1. PMID: 14592459 nitric oxide plays a pivotal role in the inflammation mediated loss in CYP1A activity in the brain. PMID: 15542068 May catalyze the oxidation of thiophene compounds with the simultaneous formation of two reactive intermediates, a thiophene-S-oxide and a thiophene epoxide. PMID: 16137656 Data show that maintenance of pulmonary CYP1A1 induction by iNO (20 ppm) + hyperoxia support the hypothesis that this phenomenon may contribute to the protective effects of inhaled nitric oxide against hyperoxic injury. PMID: 16492979 The results show that cigarette smoke (CS) and wood smoke (WS) are mutagenic but that WS differs from CS in its inability to induce pulmonary CYP1A1. PMID: 16678472 inhibition of benzo(a)pyrene-induced CYP1A1 activity with alpha-tocopherol is probably realized at the posttranslational level PMID: 16758613 effects of enteral treatment with benzo[a]pyrene on lymph node CYP 1A1/1A2 induction PMID: 16758626 Cancer preventive effect of soy-based diets is mediated in part by reduction in aryl hydrocarbon receptor protein level posttranslationally, reducing procarcinogen-induced CYP1A1 induction and metabolic activation. PMID: 17182795 results suggest that CYP1A2 plays a key role in sequence-specific 3-Methylcholanthrene-DNA adduct formation in the CYP1A1 promoter region, leading to attenuation of CYP1A1 gene expression PMID: 17276403 lack of NADPH oxidation in the presence of histamine showed that it is not a substrate for CYP1A1. Activity measurements using the O-dealkylation of ethoxyresorufin indicated that HA is a mixed-type inhibitor of CYP1A1 in both microsomes and supersomes. PMID: 17431589

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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