Recombinant Rat Calcium/Calmodulin-Dependent Protein Kinase Type Iv (CAMK4) Protein (His)

Name: Recombinant Rat Calcium/Calmodulin-Dependent Protein Kinase Type Iv (CAMK4) Protein (His)
Brand: Beta LifeScience
SKU: BLC-09980P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Rat Calcium/Calmodulin-Dependent Protein Kinase Type Iv (CAMK4) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P13234
Target Symbol	CAMK4
Synonyms	Camk4; Calcium/calmodulin-dependent protein kinase type IV; CaMK IV; EC 2.7.11.17; CaM kinase-GR; Calspermin
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MLKVTVPSCPSSPCSSVTSSTENLVPDYWIDGSKRDPLSDFFEVESELGRGATSIVYRCKQKGTQKPYALKVLKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPTEISLVLELVTGGELFDRIVEKGYYSERDAADAVKQILEAVAYLHENGIVHRDLKPENLLYATPAPDAPLKIADFGLSKIVEHQVLMKTVCGTPGYCAPEILRGCAYGPEVDMWSVGIITYILLCGFEPFYDERGDQFMFRRILNCEYYFISPWWDEVSLNAKDLVKKLIVLDPKKRLTTFQALQHPWVTGKAANFVHMDTAQKKLQEFNARRKLKAAVKAVVASSRLGSASSSHTNIQESNKASSEAQPAQDGKDKTDPLENKMQAGDHEAAKAAADETMKLQSEEVEEEEGVKEEEEEEEEEEETSRMVPQEPEDRLETDDQEMKRNSEETLKSVEEEMDPKAEEEAAAVGLGVPPQQDAILPEY
Expression Range	1-474aa
Protein Length	Full Length
Mol. Weight	57.2kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.; Heat-stable, acidic, calmodulin-binding protein.
Subcellular Location	Cytoplasm. Nucleus.
Protein Families	Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
Database References	KEGG: rno:25050 STRING: 10116.ENSRNOP00000027771 UniGene: Rn.11046
Tissue Specificity	Isoform 1 is expressed in brain and isoform 2 is testis specific.

Gene Functions References

study demonstrates that CaMKIV can relieve STZ-induced diabetic neuropathic pain.pCaMKIV can regulate the expression of High-mobility group box 1 based on both cellular and animal models. PMID: 27216039
We find that synaptic excitation and intrinsic excitability are coregulated in individual neurons through CaMKIV signaling, which is tightly controlled by neuronal activity. In contrast, synaptic inhibition is unaffected by changes in firing or CaMKIV signaling in individual neurons. PMID: 28592691
Camk4 expression in the cortical subplate was prolonged in developmentally hypothyroid pups, whereas heavy Camk4 expression in layer VIb remains at P60. Abnormal expression of Camk4 might cause altered cortical connectivity. PMID: 23680840
We have determined that high levels of CaMKIV protein are expressed by IB4-binding sensory neurons of the dorsal root ganglion PMID: 23817991
CaMKIV contributes to the diversity of dendrite arbors via restricted expression and regulation of distinct modes of dendrite elaboration. PMID: 21989476
These results suggest that NR2B-containing NMDARs have a positive role in regulating neural progenitor cell proliferation, which may be mediated through CaMKIV phosphorylation and subsequent induction of CREB activation. PMID: 21762679
Study provides correlative evidence for the neuroprotective cascade of CaMKIV/ peroxisome proliferator-activated receptor gamma coactivator-1alpha in delayed neuronal cell death and mitochondrial biogenesis in the hippocampus PMID: 20799369
abnormal regulation of CaMKIV is a component of beta-cell dysfunction caused by high glucose. PMID: 20423744
Activation of the CaMKK/CaMKIV cascade by exendin-4 stimulated ABCA1 gene transcription, indicating that exendin-4 plays an important role in insulin secretion and cholesterol ester content in pancreatic beta cells. PMID: 19874424
Camk4 is regulated directly by triiodothyronine in primary cultured neurons from fetal cortex, and the Camk4 protein is also induced by thyroid hormone. PMID: 20056827
Calcium-induced dendritic growth is regulated by activation of a transcriptional program that involves CaM kinase IV-mediated signaling to the nucleus. PMID: 12086646
demonstrated regulation of endogenous CaMKIV by T3 in embryonic stem (ES) cells differentiated into neurons and identified a single complex element in the CaMKIV 5'-flanking region that confers this regulation PMID: 12403833
Thr196 phosphorylation of CaM-KIV by CaM-KK reduces interaction of the catalytic core with the autoinhibitory region, resulting in generation of the autonomous activity PMID: 15262966
the CaM-KK-CaM-KIV cascade probably plays an important role in thyroid releasing hormone induction of thyroid stimulating hormone and prolactin transcriptional activity in pituitary cells. PMID: 15308608
study establishes nuclear m-calpain as a regulator of CaMKIV and associated signaling molecules under conditions of sustained Ca(2+) influx PMID: 15537635
Expression of active CaMKIV caused an enhancement of both NMDA receptor-mediated synaptic responses and long-term potentiation. This was accompanied by changes consistent with the generation of "silent synapses". PMID: 15748849
CaMKIV signaling stimulates vitamin D receptor (VDR)-mediated transcription by PMID: 15919723
These results suggest that a signaling mechanism that involves CaMKIV/PKD and PP2A controls YY1 function through regulation of HDAC5 and is important in the maintenance of muscle differentiation. PMID: 16822951
Lithium administration reduced CREB phosphorylation and at the same time down-regulated CaMKIV (enzymatic activity, phospho-Thr196 and protein expression level) in cell nuclei from the hippocampus. PMID: 16923323
Calcineurin A and CaMKIV triggers PGC-1alpha promoter transactivation to drive the expression of non-mitochondrial proteins. PMID: 17273866
CaMKIV activates OGT (o-linked GlcNAc transferase), and OGT has an essential role on the process of CaMKIV-dependent AP-1 activation under depolarization in neuronal cells. PMID: 18029144
Activation of calcium/calmodulin-dependent kinase IV is necessary for brain-derived neurotrophic factor (BDNF)-induced phosphorylation of CREB and subsequent block of myelin-mediated inhibition of axonal growth. PMID: 18381242
TRPC6, a member of the TRPC family, promotes hippocampal neuron dendritic growth. PMID: 18559891
analysis of regulation of calcium/calmodulin-dependent kinase IV by O-GlcNAc modification PMID: 19506079
CaMKIV is regulated by the Wnt signaling pathway. CaMKIV expression could play a role in the neuroprotective function of Wnt signaling against the Alzheimer's amyloid peptide. PMID: 19711354

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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