Recombinant Rat BTLA Protein (ECD, Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0462
Recombinant Rat BTLA Protein (ECD, Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0462
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Product Overview
Tag | Fc |
Host Species | Rat |
Accession | NP_998795.1 |
Synonym | BTLA B- and T-Lymphocyte Attenuator; B- and T-Lymphocyte-Associated Protein; CD274; BTLA |
Background | B- and T-Lymphocyte Attenuator (BTLA) is a single-pass type I membrane protein containing 1 Ig-like V-type (immunoglobulin-like) domain. BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 and CTLA4, BTLA interacts with a B7 homolog, B7H4. However, unlike PD-1 and CTLA-4, BTLA displays T-Cell inhibition via interaction with tumor necrosis family receptors (TNF-R), not just the B7 family of cell surface receptors. BTLA is a lymphocyte inhibitory receptor that inhibits lymphocytes during immune response. BTLA also is a ligand for tumor necrosis factor (receptor) superfamily, member 14 (TNFRSF14), also known as herpes virus entry mediator (HVEM). BTLA-HVEM complexes negatively regulate T-cell immune responses. |
Description | A DNA sequence encoding the rat Btla (NP_998795.1) (Ala6-Tyr183) was produced with the Fc region of mouse IgG1 at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Lys 30 |
AA Sequence | Ala6-Tyr183 |
Molecular Weight | The recombinant rat Btla consists 384 amino acids and predicts a molecular mass of 43.4 kDa. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
Bioactivity | 1. Measured by its binding ability in a functional ELISA.2. Immobilized rat BTLA-mFc at 10 ug/mL (100 uL/well) can bind mouse HVEM-Fch. The EC50 of mouse EFNA5-Fc is 6.2-14.4 ng/mL. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | Recombinnat Proteins are stable for up to 1 year from date of receipt at -70°C |
Usage | For Research Use Only |
Storage | Store recombinant protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |