Recombinant Rat Atrial Natriuretic Peptide Receptor 3 (NPR3) Protein (His-SUMO)

Name: Recombinant Rat Atrial Natriuretic Peptide Receptor 3 (NPR3) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-03995P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Rat Atrial Natriuretic Peptide Receptor 3 (NPR3) Protein (His-SUMO) is produced by our E.coli expression system. This is a extracellular protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P41740
Target Symbol	NPR3
Synonyms	Npr3; Npr-cAtrial natriuretic peptide receptor 3; Atrial natriuretic peptide clearance receptor; Atrial natriuretic peptide receptor type C; ANP-C; ANPR-C; NPR-C
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	EALAAQKIEVLVLLPRDDSYLFSLARVRPAIEYALRSVEGNGTGRKLLPPGTRFQVAYEDSDCGNRALFSLVDRVAAARGAKPDLILGPVCEYAAAPVARLASHWDLPMLSAGALAAGFQHKDTEYSHLTRVAPAYAKMGEMMLALFRHHHWSRAALLYSDDKLERNCYFTLEGVHEVFQEEGLHTSAYNFDETKDLDLDDIVRYIQGSERVVIMCASGDTIRRIMLAVHRHGMTSGDYAFFNIELFNSSSYGDGSWKRGDKHDFEAKQAYSSLQTVTLLRTAKPEFEKFSMEVKSSVEKQGLNEEDYVNMFVEGFHDAILLYVLALHEVLRAGYSKKDGGKIIQQTWNRTFEGIAGQVSIDANGDRYGDFSVVAMTDTEAGTQEVIGDYFGKEGRFKMRSNVKYPWGSLKLRIDETRIVEHTNSSPCKSCGLEESA
Expression Range	41-477aa
Protein Length	Extracellular Domain
Mol. Weight	64.9kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	ANF receptor family
Database References	KEGG: rno:25339 STRING: 10116.ENSRNOP00000025966 UniGene: PMID: 29417337 NPR3 protects cardiomyocytes from apoptosis through inhibition of cytosolic BRCA1 and TNF-alpha, which are regulators of apoptosis. PMID: 27494651 down-regulation of Npr3 gene improves the circulatory levels of ANP and antioxidant system and there by attenuates the beta-adrenoceptor over-activation mediated cardiac hypertrophic growth PMID: 27108789 Results from this study point to a role for miR-100 in the regulation of NPR3 expression, and suggest a possible therapeutic target for modulation of NP bioactivity in heart disease. PMID: 25736855 the increased expression of NPR-C (r=-0.943 and -0.837 for mRNA and protein respectively, p<0.05) and NEP (r=-0.687 and -0.823 for mRNA and protein respectively, p<0.05) were accompanied by a signi fi cant decline in CNP. PMID: 24189506 These results indicate that NPR-C activation by C-ANP4-23 attenuates the enhanced levels of cell cycle proteins through the inhibition of enhanced expression of Gialpha proteins PMID: 24155894 knockdown of NPR-A up-regulates the expression of NPR-C, Gialpha proteins, and NPR-C-linked adenylyl cyclase signalling and suggests a cross-talk between NPR-A and NPR-C. PMID: 22131352 NPR-B mRNA expression was found in both cardiac cells as well as endothelial cells. PMID: 21723350 data presented here suggest that rat NPRC is a substrate for PKA and Thr 505 located within the intracellular domain of NPRC is a likely candidate site for the phosphorylation PMID: 20564198 results demonstrate that NPRs are clearly expressed in the cells located in the ganglion cell layer including the three major subtypes of retinal ganglion cells and the expression is mainly on the membrane of somata PMID: 20304036 The data suggest that in our left ventricular hypertrophy model, the NPR-A and NPR-C receptors were increased in association to the increased BNP level. PMID: 19969282 These data suggest that natriuretic peptides may play a role in maintaining the retinal functions via interaction with NPR-C. PMID: 19878700 Modulation of ANP-C receptor signaling by endothelin-1 in A-10 smooth muscle cells PMID: 12054468 data suggest that C-type natriuretic peptide attenuates neurotransmitter efflux by a mechanism involving suppression of neuronal protein kinase C activity via an interaction with the natriuretic peptide C receptor PMID: 12488334 17-amino acid sequence (R(469) to R(485)) in the middle region of the intracellular domain of NPR-C is both necessary and sufficient for activation of G proteins and effector enzymes. PMID: 12676657 findings support that ANF exerts a stimulatory effect on pancreatic exocrine secretion mediated by NPR-C receptors coupled to the phosphoinositide pathway PMID: 12829435 participation of NPR-C receptors in C-type natriuretic peptide response PMID: 14599710 a cross-talk between ANP-C receptor-mediated adenylyl cyclase and PLC signaling pathways. PMID: 15044621 Endothelium-derived CNP is involved in the regulation of the coronary circulation, and NPR-C activation underlies the vasorelaxant activity of this peptide. Moreover, this newly defined pathway represents a protective mechanism against I/R injury. PMID: 15337698 The downregulation of ANP and NPRC in retinas of diabetic rats suggests a role for this peptide in experimental diabetic retinopathy. PMID: 15789000 discussion of neuronal regulation and function of natriuretic peptide receptor C [review] PMID: 15911073 Results show that natriuretic peptide receptor NPR-C mediates the activation of nitric oxide synthase by atrial natriuretic peptide in atria. PMID: 16712979 NPRC is a potential direct regulator of pancreatic function. PMID: 17702953 Ostn is a naturally occurring ligand of the NPR-C clearance receptor and may act to locally modulate the actions of the natriuretic system in bone by blocking the clearance action of NPR-C, thus locally elevating levels of C-type natriuretic peptide. PMID: 17951249 These results suggest that C-ANP(4-23) decreases the enhanced oxidative stress in spontaneously hypertensive rats by attenuating the enhanced expression of Gialpha proteins and also the enhanced levels of NADPH oxidase. PMID: 18162186 our results suggest the existence of a local and complex peptidergic system in the rat testis, involving ANP and its receptors that could importantly modulate the androgen biosynthesis. PMID: 18778744 Structure of the atrial natriuretic peptide receptor extracellular domain in the unbound and hormone-bound states by single-particle electron microscopy PMID: 19187227 temporal relationship follows between a NO-induced decreased expression of NPR-C and G(i)alpha proteins PMID: 19252090

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.