Recombinant Rat Acetylcholinesterase (ACHE) Protein (His)

Name: Recombinant Rat Acetylcholinesterase (ACHE) Protein (His)
Brand: Beta LifeScience
SKU: BLC-01114P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Rat Acetylcholinesterase (ACHE) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P37136
Target Symbol	ACHE
Synonyms	(AChE)
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	EGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGILDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLIWIYGGGFYSGASSLDVYDGRFLAQVEGTVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELISCLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPDALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPAHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTVEERIFAQRLMQYWTNFARTGDPNDPRDSKSPRWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL
Expression Range	32-614aa
Protein Length	Full Length of Mature Protein
Mol. Weight	68.9 kDa
Research Area	Cardiovascular
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Subcellular Location	Cell junction, synapse. Secreted. Cell membrane; Peripheral membrane protein.; [Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
Protein Families	Type-B carboxylesterase/lipase family
Database References	KEGG: rno:83817 UniGene: Rn.105879
Tissue Specificity	Has been found in central nervous system and muscle. Found in embryonic liver and spleen but not in adult liver.

Gene Functions References

sepsis decreased AChE activity by reducing its expression at the neuromuscular junction. PMID: 28849127
Paraquat-induced death of hippocampal neurons was partially mediated by AChE variants inducing and cholinergic and gultamatergic transmission disruption. PMID: 28916328
Quercetin improves memory and anxiogenic-like behavior in diabetic rats by preventing the increase in Ache activity seen in streptozocin induced diabetes. PMID: 27694000
the induction of PRiMA-linked AChE in osteoblast should be independent to classical estrogen signaling pathway. PMID: 27019979
This study showed that changes in AChE may, at least in part, to lead to memory impairment caused by galactose. Taken together, our results can help understand the etiopathology of classical galactosemia. PMID: 26948151
Acetylcholinesterase activity was increased in rats subjected to mild hyperhomocysteinemia PMID: 24590316
Hindlimb unloading (simulation of hypogravity) leads to an increase in functional activity of acetylcholinesterase on the background of reduced postsynaptic membrane area occupied by acetylcholine receptors. PMID: 25711085
Under all studied experimental conditions, offspring brain acetylcholinesterase (AChE) activity was found to be significantly decreased due to maternal hypothyroidism PMID: 24632022
AChE has an important role in modulation of early immune responses against Toxoplasma gondii infection. PMID: 24039284
Spindle-shaped AChE-rich cells in the floccular stalk project to the flocculus, nodulus and uvula, but not to the cerebellar hemispheres. PMID: 24010172
Tyrosine increased acetylcholinesterase in blood & all tested brain areas. Acute administration decreased its mRNA levels in hippocampus. Chronic administration did the same in the hippocampus. PMID: 23046746
Acetylcholinesterase inhibitor huperazine A improved, or partly reversed, the Abeta-induced damage of neurite outgrowth. PMID: 23119107
in the absence of pituitary growth hormone the postnatal proliferation of cholinergic synapses in the rat spinal cord where AChE activity is abundant, is markedly reduced PMID: 22922167
Hypothyroidism significantly reduced both specific and total AChE activity in spinal cord of rat pups PMID: 22982053
The results from the present study demonstrate a marked increase in AChE activity in all brain structures following the administration of a branched-chain amino acid pool. PMID: 22328136
This study demonistrated that AChE positive neurons were stained lightly, then during later developmental periods the staining gradually increased and achieved adult level of intensity on the day P21 in spinal cord. PMID: 22028090
During induction of the non-alcoholic fatty liver disease with CCl4 AChE activity was significantly increased compared to control in blood, liver and brain tissues. PMID: 21881966
AChE activity can act as purinergic and cholinergic markers in lymphocytes PMID: 20832780
effect of melatonin on neuroinflammation and acetylcholinesterase activity induced by LPS in rat brain PMID: 20450904
These findings suggest that the restricted localization of PRiMA-linked G4 AChE at the nmjs could be contributed by the pre-synaptic motor neuron and/or the post-synaptic muscle fiber. PMID: 19680821
the importance of post-transcriptional mechanisms in regulating AChE expression in differentiating neurons and implicate HuD as a key trans-acting factor in these events PMID: 12468554
The activity of this protein is completely suppressed by the administration of LD50 of phosphacol. PMID: 12629810
AChE is necessary for normal dendrite and axon formation in hippocampal neurones and suggest that it may also play a role in excitatory synapse development, plasticity, and remodelling. PMID: 12799140
Local increment in neuronal AChE concentration in the mammalian hippocampus, such as in amyloid deposits, may play a role in triggering neuropathological and behavioral changes such as those observed in Alzheimer disease brains PMID: 12969266
In this study we show an effect of AChE that could contribute to the increased deposition of Amyloid beta PMID: 14678761
AChE is involved in regulating cell-matrix interactions in bone PMID: 15454088
Nearly all AChE-immunoreactive neurons in the Lateral hypothalamus express immunoreactivity for either melanin concentrating hormone or for orexin PMID: 15488307
rats subjected to ovariectomy presented a significant increase in brain Na+, K+-ATPase, AChE and CAT activities, but did not change the oxidative stress parameters PMID: 15869839
Results suggest that the presence of acetylcholinesterase protein doesn't correlate with catalytic activity, and the diffuse cytoplasmic and plasma membrane localization of AChE is a property of neuronal cell types. PMID: 16052514
action of P2Y1 receptor could lead Ache gene activation in cultured cortical neurons PMID: 16429571
Lead acetate decreased brain AChE activity of rats in dose/age dependent manner. PMID: 16678265
The SH-amino acids seem to affect the hippocampal enzyme indirectly, possibly by lipid(s)-protein modifications(s) and Hci by inducing oxidative stress, since no effect was observed on pure AChE activity PMID: 16773467
Results suggest that interferon-beta may act through inhibition of acetylcholinesterase, and ethidium bromide could be considered an inhibitor of AChE activity by interfering with cholinergic neurotransmission in the different brain regions. PMID: 16871442
In the cerebellum, hyperthyroidism provoked a significant decrease in both the AChE and the Na(+), K(+)-ATPase activities PMID: 17165152
findings provide evidence for intra- and supramodal influences of the neonatal removal of retinal input on neural activity- and use-dependent modifications of cortical AChE activity PMID: 17280650
In choline-deprived (CD) newborn rats,ANTIOXIDANT STATUS and AChE and Na(+),K(+)-ATPase activities were significantly reduced by 23%, 24% and 50%, respectively, in the brains of both sexes. PMID: 17484629
Low concentrations of aspartame metabolites had no effect on AChE activity, whereas high or toxic concentrations decreased enzyme activity. PMID: 17580119
that the 55-kDa AChE may be involved in different biological processes such as neural development, tumor progression, and angiogenesis. PMID: 17606622
These findings suggest that cell surface AChE, possibly in a novel signalling complex containing APP and perlecan, contributes to a generalised mechanism for polarised membrane protrusion and migration in all adherent cells. PMID: 17948252
Repeated exposure to low dose malathion increased brain AChE activity and altered antioxidant levels. PMID: 18341513
Data show that acetylcholine esterase enzyme activity shows a significant decrease in cerebral cortex and a increase in the muscle of experimental hypoxic neonatal rats. PMID: 18406032
Although the mechanisms of action of GAA on AChE activity and on memory are unclear, these findings suggest that the accumulation of GAA found in patients with GAMT-deficiency may be one of the mechanisms involved in neural dysfunction. PMID: 18437545
Tanshinone modulates AChE and NOS proteins concentrations in the hippocampus of AD rats. This may have therapeutic potential in AD rats. PMID: 18980714
Protein CutA undergoes an unusual transfer into the secretory pathway and affects the folding, oligomerization, and secretion of acetylcholinesterase. PMID: 19049969
cell death due to paraoxon not AChE dependent PMID: 19378919
Data show that after denervation, the expression of PRiMA, AChE(T) and G(4) AChE decreased markedly in the spinal cord, and in fast- and slow-twitch muscles. PMID: 19490106

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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