Recombinant Mouse Zinc Finger Protein Helios (IKZF2) Protein (His&Myc)

Name: Recombinant Mouse Zinc Finger Protein Helios (IKZF2) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-04827P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Zinc Finger Protein Helios (IKZF2) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P81183
Target Symbol	IKZF2
Synonyms	Ikzf2; Helios; Zfpn1a2; Znfn1a2; Zinc finger protein Helios; Ikaros family zinc finger protein 2
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	METDAIDGYITCDNELSPEGEHANMAIDLTSSTPNGQHASPSHMTSTNSVKLEMQSDEECDRQPLSREDEIRGHDEGSSLEEPLIESSEVADNRKVQDLQGEGGIRLPNGKLKCDVCGMVCIGPNVLMVHKRSHTGERPFHCNQCGASFTQKGNLLRHIKLHSGEKPFKCPFCSYACRRRDALTGHLRTHSVGKPHKCNYCGRSYKQRSSLEEHKERCHNYLQNVSMEAAGQVMSHHVPPMEDCKEQEPIMDNNISLVPFERPAVIEKLTANMGKRKSSTPQKFVGEKLMRFSYPDIHFDMNLTYEKEAELMQSHMMDQAINNAITYLGAEALHPLMQHAPSTIAEVAPVISSAYSQVYHPNRIERPISRETSDSHENNMDGPISLIRPKSRPQEREASPSNSCLDSTDSESSHDDRQSYQGNPALNPKRKQSPAYMKEDVKALDATKAPKGSLKDIYKVFNGEGEQIRAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRSQDRYEFSSHIVRGEHTFH
Expression Range	1-526aa
Protein Length	Full Length
Mol. Weight	66.8 kDa
Research Area	Epigenetics And Nuclear Signaling
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Associates with Ikaros at centromeric heterochromatin.
Subcellular Location	Nucleus.
Protein Families	Ikaros C2H2-type zinc-finger protein family
Database References	KEGG: mmu:22779 STRING: 10090.ENSMUSP00000027146 UniGene: PMID: 29440380 He is involved in the correct development of a subset of striatopallidal medium spiny neurons. PMID: 28289129 Helios controls certain aspects of Treg-suppressive function, differentiation, and survival. PMID: 26582951 this study reports that impaired expression of the transcription factor Helios by FoxP3+ CD4 and Qa-1-restricted CD8 Tregs results in defective regulatory activity and autoimmunity in mice. PMID: 26472910 data suggest that a subset of DN T cells, identified by the expression of PD-1 and Helios, represent self-reactive cells; results provide an explanation for the origin of DN T cells and introduce CD8 loss as a process associated with self-Ag encounter PMID: 25825451 results demonstrate that Helios is expressed by thymic and peripheral T cells that are being driven to tolerance in response to a genuine autoantigen PMID: 24740292 identify Helios as the first marker known to diverge during positive and negative selection of thymocytes PMID: 23337809 Helios regulates IL-2 production in Tregs by suppressing Il2 gene transcription. Loss of Helios in Tregs breaks their anergic phenotype and results in derepression of the Il2 locus. PMID: 23275607 Helios expression spatially coincides with the early pattern of expression of the mature markers of striatal projection neurons. PMID: 22142223 In tumor-bearing mice, Helios(+) regulatory T cells were relatively over-represented in the tumor-mass. PMID: 22479644 Data found that Helios-negative T cells are enriched for naive T cell phenotypes and vice versa. Moreover, Helios can be induced during T cell activation and proliferation, but regresses in the same cells under resting conditions. PMID: 21918685 down-regulation of NK cell activity by NKp46 was associated with the silencing of the Helios transcription factor in NK cells PMID: 22267813 show that Helios is upregulated in CD4(+)CD25(+) regulatory T cells. PMID: 20226531 silencing of Helios is critical for normal B cell function PMID: 16148093 results demonstrate a vital function for Helios in maintaining normal homeostasis of developing T cells and formally show that non-DNA-binding isoforms of Helios are lymphomagenic if aberrantly expressed within the T-cell lineage PMID: 17110463 Helios is not essential for T cells; its function can be compensated for by other members of the Ikaros family. PMID: 19620299

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.