Recombinant Mouse Wee1-Like Protein Kinase (WEE1) Protein (His)

Name: Recombinant Mouse Wee1-Like Protein Kinase (WEE1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-07348P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse Wee1-Like Protein Kinase (WEE1) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P47810
Target Symbol	WEE1
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MSFLSRQQPPPTRRVGAAYSLRQKLIFSPGSDCEEEEEEEEEGSGHSTGEDSAFQEPDSPLPSARSPAEAEAERRRRSPGAEPSSPGELEDDLLLQGGGGGAQAAGGGAEGDSWEEEGFGSSSPVKSPSTAYFLSSPFSPVRCGGPGDASPQGCGAPRAMDDPCSPQPDYPSTPPHKTFRKLRLFDTPHTPKSLLSKARVIDSGSVKLRGSSLFMDTEKSGKREFDTRQTPQVNINPFTPDPVLLHSSGRCRGRKRAYFNDSSEDMEASDYEFEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHPHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRVMSYLTEVELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAVSEEGDEDDWISNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYSHLPKADIFALALTVVCAAGAEPLPRNGEQWHEIRQGRLPRIPQVLSQEVTELLRVMIHPDPERRPSAMELVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAAKVAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY
Expression Range	1-646aa
Protein Length	Full Length
Mol. Weight	77.5 kDa
Research Area	Cell Biology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Subcellular Location	Nucleus.
Protein Families	Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily
Database References	KEGG: mmu:22390 STRING: 10090.ENSMUSP00000033326 UniGene: Mm.287173

Gene Functions References

Morphine significantly downregulated the expression of miRNA-219-5p, which targets WEE1 to suppress Tyr15 expressions and activate Cdc2, thus inhibiting the morphine-induced macrophage apoptosis. PMID: 27253431
Results revealed that WEE1 is indispensable for maintaining genomic stability and it functions as a haploinsufficient tumor suppressor. PMID: 25088202
Wee1 is directly or indirectly involved in the mechanism of the circadian rhythm-dependent changes in docetaxel-induced intestinal damage. PMID: 23892568
The induction of Wee1 under hypoxia was confirmed both at the mRNA and protein levels. PMID: 21918363
In this study, we found that the chromosomal wee1 gene is also down-regulated by KLF3. PMID: 22115574
The regulation of Wee1 by SadA and SadB kinases is essential for the differentiation of polarized neurons. PMID: 20026642
Mammalian Wee1 plays a critical role in maintaining genome integrity and is essential for embryonic survival at the pre-implantation stage of mouse development. PMID: 16810330
expression of wee1 was directly regulated by the molecular components of the circadian clockwork in the regenerating liver PMID: 12934012
transcription repressive activity of TBP-related factor 2 to wee1 promoter needs association with the promoter and TFIIA PMID: 17109819
the suppression of nuclear import of cyclin B1, the induction of Wee1 kinase activity, and the transient nuclear accumulation of p21(CIP1/WAF1) may play important roles in the transient cell cycle delay in response to ionizing radiation PMID: 18425394

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.