Recombinant Mouse Tsukushi (TSKU) Protein (His&Myc)
Beta LifeScience
SKU/CAT #: BLC-00655P
Greater than 90% as determined by SDS-PAGE.
Recombinant Mouse Tsukushi (TSKU) Protein (His&Myc)
Beta LifeScience
SKU/CAT #: BLC-00655P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
| Description | Recombinant Mouse Tsukushi (TSKU) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Uniprotkb | Q8CBR6 |
| Target Symbol | TSKU |
| Synonyms | (Leucine-rich repeat-containing protein 54) |
| Species | Mus musculus (Mouse) |
| Expression System | E.coli |
| Tag | N-10His&C-Myc |
| Target Protein Sequence | TRPCFPGCQCEEETFGLFDSFSLIRVDCSSLGPHIVPVPIPLDTAHLDLSSNRLETVNESVLAGPGYTTLAGLDLSYNLLTSIMPSAFSRLRYLESLDLSHNGLAALPAEIFTSSPLSDINLSHNRLREVSISAFTTHSQGRALHVDLSHNLIHRLLPHPARASLPAPTIQSLNLSWNRFRAVPDLRDLPLRYLSLDGNPLATINPDAFMGLAGLTHLSLASLQGILHLPPHGFRELPGLQVLDLSGNPKLKWAGAEVFSGLGLLQELDLSGSSLVPLPEMLLHHLPALQSVSVGQDVQCRRLVREGAYHRQPGSSPKVVLHCGDTQESAARGPDIL |
| Expression Range | 18-354aa |
| Protein Length | Full Length of Mature Protein |
| Mol. Weight | 44.0 kDa |
| Research Area | Others |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
| Target Function | Contributes to various developmental events and other processes such as wound healing and cholesterol homeostasis through its interactions with multiple signaling pathways. Wnt signaling inhibitor which competes with WNT2B for binding to Wnt receptor FZD4 and represses WNT2B-dependent development of the peripheral eye. Plays a role in regulating the hair cycle by controlling TGFB1 signaling. Required for the development of the anterior commissure in the brain by inhibiting neurite outgrowth. Essential for terminal differentiation of hippocampal neural stem cells. Plays a role in regulating bone elongation and bone mass by modulating growth plate chondrocyte function and overall body size. Required for development of the inner ear through its involvement in stereocilia formation in inner hair cells. Facilitates wound healing by inhibiting secretion of TGFB1 from macrophages which prevents myofibroblast differentiation, maintaining inflammatory cell quiescence. Plays a role in cholesterol homeostasis by reducing circulating high-density lipoprotein cholesterol, lowering cholesterol efflux capacity and decreasing cholesterol-to-bile acid conversion in the liver. In one study, shown to negatively regulate sympathetic innervation in brown fat, leading to reduced energy expenditure. In another study, shown not to affect brown fat thermogenic capacity, body weight gain or glucose homeostasis. |
| Subcellular Location | Secreted. |
| Database References | KEGG: mmu:244152 STRING: 10090.ENSMUSP00000091713 UniGene: PMID: 22995554 |
