Recombinant Mouse Tryptophan 5-Hydroxylase 1 (TPH1) Protein (His-SUMO)

Beta LifeScience SKU/CAT #: BLC-04023P
Greater than 90% as determined by SDS-PAGE.
Greater than 90% as determined by SDS-PAGE.

Recombinant Mouse Tryptophan 5-Hydroxylase 1 (TPH1) Protein (His-SUMO)

Beta LifeScience SKU/CAT #: BLC-04023P
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Product Overview

Description Recombinant Mouse Tryptophan 5-Hydroxylase 1 (TPH1) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity Greater than 90% as determined by SDS-PAGE.
Uniprotkb P17532
Target Symbol TPH1
Synonyms Tph1; Tph; Tryptophan 5-hydroxylase 1; EC 1.14.16.4; Tryptophan 5-monooxygenase 1
Species Mus musculus (Mouse)
Expression System E.coli
Tag N-6His-SUMO
Target Protein Sequence MIEDNKENKENKDHSSERGRVTLIFSLENEVGGLIKVLKIFQENHVSLLHIESRKSKQRNSEFEIFVDCDISREQLNDIFPLLKSHATVLSVDSPDQLTAKEDVMETVPWFPKKISDLDFCANRVLLYGSELDADHPGFKDNVYRRRRKYFAELAMNYKHGDPIPKIEFTEEEIKTWGTIFRELNKLYPTHACREYLRNLPLLSKYCGYREDNIPQLEDVSNFLKERTGFSIRPVAGYLSPRDFLSGLAFRVFHCTQYVRHSSDPLYTPEPDTCHELLGHVPLLAEPSFAQFSQEIGLASLGASEETVQKLATCYFFTVEFGLCKQDGQLRVFGAGLLSSISELKHALSGHAKVKPFDPKIACKQECLITSFQDVYFVSESFEDAKEKMREFAKTVKRPFGLKYNPYTQSVQVLRDTKSITSAMNELRYDLDVISDALARVTRWPSV
Expression Range 1-447aa
Protein Length Full Length
Mol. Weight 67.3kDa
Research Area Signal Transduction
Form Liquid or Lyophilized powder
Buffer Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis.
Protein Families Biopterin-dependent aromatic amino acid hydroxylase family
Database References

KEGG: mmu:21990

STRING: 10090.ENSMUSP00000037752

UniGene: PMID: 30446669

  • Data suggest that central melanocortin-4 receptor (MC4-R) and tryptophan hydroxylase (TPH) are involved in the efferent neuronal control of the kidneys. PMID: 27626491
  • Prolactin-induced Tph1 expression. PMID: 26136513
  • Tph1-deficient mice fed a high-fat diet (HFD) are protected from obesity, insulin resistance and nonalcoholic fatty liver disease (NAFLD) while exhibiting greater energy expenditure by brown adipose tissue. PMID: 25485911
  • Lack of tryptophan hydroxylase-1 in mice results in gait abnormalities. PMID: 23516593
  • ZBP-89 directly binds to the Tph1 promoter, which is required for its basal and butyrate-inducible expression. PMID: 23395646
  • TPH1 deficiency or inhibition reduces allergic airway inflammation. Platelet-derived 5-HT is pivotal in AAI and lack of 5-HT leads to impaired Th2 priming capacity of bone marrow dendritic cells. PMID: 23328530
  • Data indicate that tryptophan hydroxylase (Tph)1-deficient mice, lacking non-neuronal serotonin, showed mild leukocytosis. PMID: 23243271
  • Data show that Tph-1 (tryptophan hydroxylase-1) is a potent regulator of immunity. PMID: 23008335
  • Parallel to Tph1 mRNA and protein induction, islet serotonin content increased to a peak level that was 200-fold higher than basal. Interestingly, only a subpopulation of the beta cells was serotonin-positive in vitro and in vivo. PMID: 20938637
  • nonneuronal tph1 gene has a role in cardiac function with peripheral serotonin PMID: 14597720
  • Review. TPH1 is expressed in the pineal gland and the enterochromaffin cells. Loss of TPH1 gene expression, and thus of peripheral 5-HT, leads to a cardiac dysfunction phenotype. PMID: 15146950
  • estrogen receptor-beta is responsible for mediating estrogen regulated tryptophan hydroxylase-1 expression in the murine dorsal raphe nucleus PMID: 15820717
  • TPH1 is expressed preferentially during late developmental brain stages. Low raphe nuclei 5-HT contents were seen during development in NZW & SWR mice with common functional polymorphisms in TPH1. TPH1 may influence behavior later in life. PMID: 16407550
  • TPH1 is the predominant form expressed in pineal gland and with a molecular weight of 51 kDa. PMID: 16581041
  • Tryptophan hydroxylase 1 and peripheral serotonin play an essential role in the development of hypoxia-induced elevations in pulmonary pressures and hypoxia-induced pulmonary vascular remodeling. PMID: 17130306
  • Tph1 knockout mice used to show that serotonin plays a role in the pathogenesis of steatohepatitis. PMID: 17681180
  • Cardiomyopathy is more severe in adult Tph1 knockout mice born to homozygous mothers than to heterozygous mothers. PMID: 18267982
  • In a genetic model of serotonin deficiency (Tph1(-/-)) in mice, serotonin shown to be crucial for the growth of s.c. colon cancer allografts in vivo. PMID: 18593914
  • substantially reduced peripheral 5-HT levels in Tph1/Tph2 double knockout, but not TPH2KO mice PMID: 18923670
  • In mice no significant TPH1 expression was detected in the brain. PMID: 19181488

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    Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

    Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

    Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

    Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

    To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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