Recombinant Mouse Tribbles Homolog 3 (TRIB3) Protein (His&Myc)

Name: Recombinant Mouse Tribbles Homolog 3 (TRIB3) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-00829P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Tribbles Homolog 3 (TRIB3) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q8K4K2
Target Symbol	TRIB3
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MRATPLAASADVSCRKKPLEFDDNIDAKCPVLKRVRDEPEPGPLPSLLPPSPPPASDLSPAVAPATRLGPYILLEREQGSCSYRALHCPTGTEYTCKVYPASEAQAVLAPYARLPTHQHVARPTEVLLGSRLLYIFFTKTHGDLHSLVRSRRGIPESEAAGLFRQMASAVAHCHKHGLVLRDLKLRRFVFSNCERTKLVLENLEDACVMTGSDDSLWDKHACPAYVGPEILSSRPSYSGKAADVWSLGVALFTMLAGRYPFHDSEPVLLFGKIRRGTFALPEGLSAPARCLIRCLLRKEPSERLVALGILLHPWLREDHGRVSPPQSDRREMDQVVPDGPQLEEAEEGEVGLYG
Expression Range	1-354aa
Protein Length	Full Length
Mol. Weight	46.5 kDa
Research Area	Cardiovascular
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity. Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. Can inhibit APOBEC3A editing of nuclear DNA.
Subcellular Location	Nucleus.
Protein Families	Protein kinase superfamily, CAMK Ser/Thr protein kinase family, Tribbles subfamily
Database References	KEGG: mmu:228775 STRING: 10090.ENSMUSP00000041747 UniGene: PMID: 28117339 Overexpressed Trib3, specifically in skeletal muscle tissues, displayed impaired glucose homeostasis by suppressing insulin-stimulated glucose uptake. PMID: 30071535 under nutrient-limiting conditions that stimulate ATF4 activity, TRIB3 is implicated in the regulation of metabolic adaptation by restraining the transcription of Fgf21. PMID: 29378327 Data (including data from studies in transgenic and mutant mice) suggest that Akt2 plays critical role in type 1 diabetes in metallothionein-mediated preservation of cardiac insulin-stimulated metabolic signaling (insulin resistance) and preservation of cardiac function; this mechanism appear to involve inhibition of cardiac Trb3. (Akt2 = protein kinase Akt-2; Trb3 = tribbles pseudokinase 3) PMID: 29079702 restoration of TRB3 expression markedly abolished the effect of miR-124 on hepatic TG metabolism. PMID: 27845424 TRIB3, a single-domain protein with impaired kinase catalytic activity, has been described to perform a complex of roles, many of which are likely to be cell type-specific. (Review) PMID: 28393700 TRB3 regulates protein synthesis and breakdown via the Akt/mTOR/FoxO pathways in skeletal muscle. PMID: 28962861 Under physiological conditions, muscle TRIB3 also influences energy expenditure and substrate metabolism, indicating that the decrease and increase in muscle TRIB3 under fasting and nutrient excess, respectively, are critical for metabolic homeostasis. PMID: 27207527 In a knockout mouse model, Trib3 disruption modestly increased steady-state erythrocyte numbers and decreased mean corpuscular volume. PMID: 28062363 These data demonstrate that TRB3 inhibits insulin signaling in BAT, resulting in impaired differentiation and function. PMID: 26801556 TRB3 is a novel profibrotic mediator in SSc. TGF-beta induces TRB3, which in turn activates canonical TGF-beta/Smad signaling and stimulates the release of collagen, creating a positive feedback loop that may contribute to aberrant TGF-beta signalling in SSc. PMID: 25603829 study presents the finding that activation of the NF-kappaB pathway by Porphyromonas endodontalis LPS is important for Wnt5a expression in osteoblasts, and that TRIB3 may modulate the sustained expression of Wnt5a in osteoblasts stimulated by P. endodontalis PMID: 25601649 Trib3 interferes with the Parkinson's disease-associated prosurvival protein Parkin to mediate death PMID: 26224857 These observations support the notion that loss of TRIB3 is associated with a more aggressive phenotype in various types of tumors by enhancing the activity of the mTORC2/AKT/FOXO axis. PMID: 25168244 Tribbles 3 regulates the fibrosis cytokine TGF- beta 1 through ERK1/2-MAPK signaling pathway in diabetic nephropathy. PMID: 25133193 Fibroblasts from two long-lived mice mutants (Snell dwarf and PAPP-A knockout) expressed higher levels of TRB3 (a ATF4 target gene) during stress, suggesting a connection to longevity. PMID: 24691093 TRB3(-/-) islets show a decrease in both the amplitude and duration of cytokine-stimulated MLK3 induction and JNK activation. PMID: 25204656 Trib3 is upregulated in the mouse anterior piriform cortex in response to leucine-deficient diet. Deletion of Trib3 does not influence the rejection of amino acid-imbalanced diet. Trib3 is dispensable for spatial and reversal learning. PMID: 24732777 These findings suggest that TRB3 was involved in lipoapoptosis of INS-1 beta cell PMID: 24824999 show in renal tubular cells that TRB3 binds to mTOR and the rapamycin-insensitive companion of mTOR (Rictor), a protein specific to mTORC2 PMID: 24676635 TRB3 regulates muscle fiber type via a peroxisome proliferator-activated receptor-alpha (PPAR-alpha)-regulated miR499/miR208b pathway, revealing a novel function for TRB3 in the regulation of skeletal muscle fiber type and exercise capacity. PMID: 24740654 Data indicate that responding to Toll-like receptor 2 (TLR2)-specific stimulation, the expressions of cell death inducible putative kinase (NIPK) was significantly elevated. PMID: 25108431 TRIB3 mediated ER stress-induced beta-cell apoptosis via the NF-kappaB pathway. PMID: 24746137 observations presented here strongly support that TRIB3 plays a crucial role on THC anti-neoplastic activity PMID: 23567453 Trib3 mRNA expression in bone-marrow-derived mast cells is upregulated by IL-3. Degranulation & activation-induced cytokine mRNA expression are impaired in Trib3-deficient BMMCs. PMID: 23261831 TRB3 increased in diabetic testis and further increased in the testis of diabetic mice with Zn deficiency. PMID: 22000581 Silence of TRIB3 would diminish atherosclerotic burden and increase the plaque stability in diabetic mice. PMID: 22275087 emodin induces BV-2 cell apoptosis through TRB3 and consequently eliminates inflammatory microglia. PMID: 21275776 HHcy impairs hepatic glycogen synthesis by inducing the expression of TRB3 PMID: 21435438 the GCN2/eIF2alpha/ATF4 pathway is essential for the induction of the TRB3 gene transcription PMID: 21203563 enhanced reactive oxygen species and/or free fatty acids associated with the diabetic milieu induce podocyte C/EBP homologous protein and TRB3 expression PMID: 20660016 The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells. PMID: 20592469 MLK3 mediates its effects via the pseudokinase TRB3, a mammalian homolog of Drosophila Tribbles. PMID: 20421299 These data identify TRIB3 induction as a novel molecular mechanism in human insulin resistance and diabetes. PMID: 19996382 acute exercise reverses TRB3 expression and insulin signalling restoration in muscle PMID: 19681769 Knockdown of hepatic TRB-3 expression improved glucose tolerance, whereas hepatic overexpression of TRB-3 reversed the insulin-sensitive phenotype of PGC-1-deficient mice. PMID: 15107844 The combination of elevated TRB3 and constitutive hepatic p70 S6 kinase activity results in decreased insulin signaling via the insulin receptor substrate-1 protein/phosphatidylinositol 3-kinase/Akt pathway. PMID: 16887816 Results indicated that TRB3 plays a critical role in muscle differentiation. PMID: 17207467 Deletion of Trib3 has minimal effect on insulin-induced Akt activation in hepatic tissue. PMID: 17303803 Data show that TRB3 is an important negative regulator of adipogenesis that acts at an early step in the differentiation cascade to block the C/EBPbeta proadipogenic function. PMID: 17646392 TRB3 acts as a potent negative regulator of PPARgamma, a master regulator of adipocyte differentiation, and tightly controls adipogenesis PMID: 18187772 Data show that C/EBPbeta expression induced by insulin preceded that of TRB3 and that mutation of the C/EBPbeta binding site in TRB3 promoter abolished the responsiveness of the TRB3 gene to insulin. PMID: 19164449 TRB3 expression was reduced in the hepatic tissue from diet-induced obesity (Swiss) and leptin-deficient (ob/ob) mice, when compared with respective control groups at rest. PMID: 19492410 PPARalpha ligands may reduce cellular number by augmenting TRB3 expression, which in turn induces cell cycle arrest by reducing the expression of Cyclin B1. PMID: 19748123

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Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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