Recombinant Mouse Transcription Factor Gata-4 (GATA4) Protein (His&Myc)

Name: Recombinant Mouse Transcription Factor Gata-4 (GATA4) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-08010P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse Transcription Factor Gata-4 (GATA4) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q08369
Target Symbol	GATA4
Synonyms	Gata4; Gata-4; Transcription factor GATA-4; GATA-binding factor 4
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MYQSLAMAANHGPPPGAYEAGGPGAFMHSAGAASSPVYVPTPRVPSSVLGLSYLQGGGSAAAAGTTSGGSSGAGPSGAGPGTQQGSPGWSQAGAEGAAYTPPPVSPRFSFPGTTGSLAAAAAAAAAREAAAYGSGGGAAGAGLAGREQYGRPGFAGSYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRANPGRHPNLDMFDDFSEGRECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRRLSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAGPAGETLPPSSGASSGNSSNATSSSSSSEEMRPIKTEPGLSSHYGHSSSMSQTFSTVSGHGPSIHPVLSALKLSPQGYASPVTQTSQASSKQDSWNSLVLADSHGDIITA
Expression Range	1-441aa
Protein Length	Full Length
Mol. Weight	49.6 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase.
Subcellular Location	Nucleus.
Database References	KEGG: mmu:14463 STRING: 10090.ENSMUSP00000113891 UniGene: Mm.247669
Tissue Specificity	Heart, intestine, liver, primative endoderm and gonads.

Gene Functions References

direct binding of GATA4 to the GNAI3 promoter, both in vitro and in vivo, is reported. PMID: 28484278
Downregulation of Gata4 could restore the phenotype exhibited by Wnt3 downregulation in dorsal root ganglion neurons. PMID: 29567480
Enrichment of induced cardiomyocytes derived from mouse fibroblasts can be achieved by reprogramming with cardiac transcription factors, Gata4, MEF2c, Tbx5, and Hand2. PMID: 29257325
GATA4/6 are key regulators of steroidogenesis and testicular somatic cell survival. PMID: 28710293
Observations identify Gata4 as a novel crucial regulator of the intestinal epithelial barrier and as a critical epithelial transcription factor implicated in the maintenance of proximal intestinal mucosal integrity after injury. PMID: 27827449
Gata4 mutants also demonstrated Hedgehog (Hh) signaling defects. PMID: 28167794
disruption of GATA4-mediated transactivation in hepatocellular carcinoma suppresses hepatocyte epithelial differentiation to sustain replicative precursor phenotype PMID: 28758902
Mechanistically, decreased GATA4 levels caused the downregulation of several pro-regenerative genes (among them interleukin-13, Il13) in the myocardium. PMID: 28053183
GATA4 acts as master regulator of hepatic microvascular specification and acquisition of organ-specific vascular competence, which are indispensable for liver development. PMID: 28218627
study provides novel insights into the role of WT1 and GATA4 during the sex differentiation phase and represents an approach that can be applied to assess other proteins with as yet unknown functions during gonadal development PMID: 28426816
we have identified a distinct lineage of adult HSCs characterized by its derivation of progenitors where Gata4 expression is activated by a specific enhancer. Most adult HSCs belonging to this lineage probably originate in the placenta. PMID: 28057738
Histone acetylation/methylation and DNA methylation were both involved in regulating GATA4 expression, but Nkx2.5 expression was not regulated by DNA methylation. These three modifications had high correlation with each other during regulation of GATA4 and produced a regulation loop at the GATA4 promoter. PMID: 27117983
GATA4 acts as a negative regulator of Bsp expression in osteoblasts. PMID: 26973342
Detailed analysis of specific lineage markers expression showed selective downregulation of endoderm markers in REST-null cells, thus contributing to a loss of cardiogenic signals. REST regulates cardiac differentiation of ESCs by negatively regulating the Wnt/beta-catenin signaling pathway and positively regulating the cardiogenic TF Gata4 PMID: 26864965
GATA4 and GATA6 are essential for female fertility, whereas targeting either factor alone causes subfertility. GATA4 and GATA6 are also required for the expression of the receptors for prolactin and luteinizing hormone. PMID: 26510866
Loss of Gata4 in Sertoli cells impairs the spermatogonial stem cell niche and causes germ cell exhaustion by attenuating chemokine signaling. PMID: 26473289
data support the concept that under physiological conditions microbiota stimulate Gata4, which suppresses Asbt expression PMID: 26022694
MITF interacts with BRG1 to promote GATA4 expression in cardiac hypertrophy. PMID: 26388265
Data show that three transcriptional factors Gata4, Mef2c, and Tbx5 (abbreviated as GMT) significantly improved murine embryonic stem cells (ESCs) differentiated into cardiomyocytes. PMID: 26449528
the data suggest that the inhibitory effects of melatonin on testosterone production are mediated via down-regulation of GATA-4 and SF-1 expression. PMID: 26386639
GATA4/6-mediated inhibition of hedgehog signaling is a major mechanism regulating pancreatic endoderm specification during patterning of the gut tube PMID: 26932670
GATA4 is required for neonatal heart regeneration through regulation of Fgf16. PMID: 26893347
Study reports extensive and complex interdependent genomic occupancy of TBX5, NKX2-5, and the zinc finger TF GATA4 coordinately controlling cardiac gene expression, differentiation, and morphogenesis. PMID: 26875865
studies suggest that Gata4 has evolved distinct functions in the development of these tissues that cannot be performed by Gata6, even when it is provided in the identical expression domain PMID: 26687508
The model proposed will help to elucidate the molecular basis for disease causing mutations in GATA4 and NKX2-5 and may be relevant to other members of the GATA and NK classes of transcription factors. PMID: 26642209
GATA4 has been shown to be a positive regulator of steroidogenic genes, including STAR protein, P450 aromatase, and 3B-hydroxysteroid dehydrogenase PMID: 26183893
The role of GATA4 in the regulation of cardiomyocyte maturation in the newborn heart and in maintaining diastolic function in the adult heart. PMID: 26023924
Conditional Gata4 deletion in mouse intestinal epithelium supported its regulatory role for Reg1, Reg3alpha, Reg3beta and Reg3gamma genes. Reg1 down-modulation of expression in Gata4 conditional null mice decreased intestinal epithelial cell migration. PMID: 26477491
our results suggest that activation of NHE1 induces hypertrophy through the activation of NFAT3/Gata4 and OPN expression PMID: 25758355
this study confirms that GATA4 M310V mutation may lead to the development of the congenital heart defect, ASD. PMID: 25873328
These data therefore provide strong evidence that GATA4 is an essential transcription factor that sits atop of the Leydig cell steroidogenic program. PMID: 25504870
Mice with double deletion of Gata4 and Gata6 genes lack identifiable adrenal glands, steroidogenic factor 1-positive cortical cells and steroidogenic gene expression in the adrenal location. PMID: 25933105
GATA6 likely regulates enteroendocrine cell differentiation cell autonomously whereas GATA4 affects this population indirectly PMID: 25495347
Microarray analysis revealed GATA4 suppression of TGFbeta signaling, necessary for osteoblast progenitor maintenance, and concomitant activation of BMP signaling, necessary for mineralization. PMID: 24932701
Combined loss of GATA4 and GATA6 leads to a lack of testis functionality, with a loss of normal steroidogenic testis function, concomitant with an expansion of the adrenal-like cell population in postnatal conditional double mutant testes. PMID: 25668066
GATA4 plays a pivotal role in regulation of Leydig cell glycolysis and androgen biosynthesis. PMID: 25668067
During cardiac differentiation, GATA-4 forms a complex with STAT3 to bind to the EGF promoter in response to EGF stimulation. PMID: 25504289
Disruption of myocardial Gata4 and Tbx5 results in defects in cardiomyocyte proliferation and atrioventricular septation. PMID: 24858909
NEXN as a novel gene for ASD and its function to inhibit GATA4 established a critical regulation of an F-actin binding protein on a transcription factor in cardiac development PMID: 24866383
GATA4 interacted with Dlx5 and subsequently decreased Dlx5 binding activity to Runx2 promoter region. PMID: 24355298
MyoR modulates cardiac conduction by repressing Gata4. PMID: 25487574
GATA4 and GATA6 proteins are fundamental regulators of granulosa cell differentiation and proliferation, and consequently of proper follicular assembly during normal ovarian development and function. PMID: 24899573
GATA4 represses an ileal program of gene expression in the proximal small intestine by inhibiting the acetylation of H3K27. PMID: 24878542
GATA transcription factors are repressors of hedgehog signaling, and NKX3.2 maintains the ability of sclerotomal cells to express SHH transcriptional targets in the presence of BMP signals by repressing the induction of Gata4/5/6 PMID: 25294942
Gata4 promoter 1b-directed expression is regulated by GATA4 itself. Thus, Gata4 transcription in the gonads and other tissues is ensured by distinct promoters that are regulated differentially and independently. PMID: 24352556
SRC-2 is critical to transcriptional control modulated by MEF2, GATA-4, and Tbx5, thereby enhancing gene expression associated with cardiac growth. PMID: 24811170
GATA4 and GATA6 play an essential role in maintaining proper intestinal epithelial structure and in regulating intestinal epithelial cytodifferentiation. PMID: 24929016
GATA-4 and GATA-6 play a dosage-dependent and redundant role in programming cardiac hypertrophy. PMID: 24391969
Data indicate that Nkx2-5, Tbx5, Gata4, or Myocd alone did not induce the de novo expression of cardiac marker proteins in 10T1/2 non-myoblastic cells. PMID: 23144723
Mesenchymal GATA4 activity regulates hepatic stellate cell activation and inhibits the liver fibrogenic process. PMID: 24415412

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