Recombinant Mouse TRANCE/RANKL Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0035
Recombinant Mouse TRANCE/RANKL (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 19 kDa and purity greater than 96%.
Recombinant Mouse TRANCE/RANKL (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 19 kDa and purity greater than 96%.

Recombinant Mouse TRANCE/RANKL Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0035
/
Size

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Product Overview

Product Name Recombinant Mouse TRANCE/RANKL Protein
Target Symbol TNFSF11
Alternative Names Tumor Necrosis Factor Ligand Superfamily Member 11, TNFSF11, TNF-Related Activation-Induced Cytokine, Osteoclast Differentiation Factor, Receptor Activator Of Nuclear Factor Kappa B Ligand, Tumor Necrosis Factor Ligand 6B, Osteoprotegerin Ligand, TRANCE, RANKL, OPGL, ODF, CD254, HRANKL2 , TNLG6B, OPTB2, SOdf
Accession Number O35235
Expression System E. coli.
Molecular Weight 19.3 kDa (monomer, predicted)
Sequence Ala144-Asp316
Purity >96% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
Activity Determined by an osteoclast differentiation assay using mouse RAW 264.7 cells. The ED50 for this effect is typically 1-10 ng/mL
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4, without carrier-protein.
Endotoxin Level <0.5 EU per 1 µg of the protein as determined by the LAL method
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function TRANCE (TNF-related activation-induced cytokines) also known as RANKL (Receptor Activator of Nuclear Factor Kappa-B Ligand), is a protein primarily involved in the regulation of bone remodeling and immune function. Its main function is to stimulate the differentiation and activation of osteoclasts. Expression of TRANCE by T cells promotes survival and activation of dendritic cells. Development of the lymph node stromal cells involves TRANCE and regulation of self-tolerance in the thymus also involves TRANCE. Additional functions of TRANCE include: differentiation of lactating mammary epithelial cells, glucose metabolism in the liver, tumorigenesis and metastasis. TRANCE signaling is facilitated through binding its receptor, RANK (CD265). However, Osteoprotegerin (OPG) acts as a decoy receptor for TRANCE, thereby regulating TRANCE-RANK interactions.
Tissue Specificity TRANCE expression has been reported in primarily in lungs, thymus and lymph nodes, and also in bone marrow, stomach, spleen, breast, placenta, heart, thyroid, and skeletal muscle.
Cellular Localization Membrane-bound and cleaved soluble protein forms
Involvement In Disease Dysregulation of TRANCE is related to osteoporosis, rheumatoid arthritis (RA), Paget’s disease (chronic bone disorder characterized by abnormal bone remodeling), bone loss in periodontal disease, vascular calcification, multiple sclerosis (MS), systemic lupus erythematosus (SLE), multiple myeloma, breast cancer, lung cancer and bone metastases of tumors.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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