Mouse Tlr4 (Toll-Like Receptor 4) - Recombinant Protein

Beta LifeScience SKU/CAT #: BLT-08545P
SDS-PAGE analysis of Mouse Tlr4 (Toll-Like Receptor 4) - Recombinant Protein, CAT
SDS-PAGE analysis of Mouse Tlr4 (Toll-Like Receptor 4) - Recombinant Protein, CAT# BLT-08545P showing >90% purity under 15% SDS-PAGE (Reduced)

Mouse Tlr4 (Toll-Like Receptor 4) - Recombinant Protein

Beta LifeScience SKU/CAT #: BLT-08545P
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Size

Quantity Pricing

Pack Size Price (USD)
500 µg $962.50 Original price: $1,375.00
1 mg $1,746.00 Original price: $2,495.00

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Product Overview

Product Name Recombinant Mouse Toll Like Receptor 4 (TLR4) Protein
Product Overview This recombinant mouse Toll Like Receptor 4 (TLR4) protein includes amino acids 26-269 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization.
Target Uniprot Id Q9QUK6
Recommended Name Toll-like receptor 4
Gene Name Tlr4
Synonyms CD284; TOLL; HToll
Species Mouse
Predicted Molecular Mass 32 kDa
Expression System E.coli
Expression Range 26-269
Tag N-6His
Purity >90%
Formulation Lyophilized
Buffer PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol
Storage Condition 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Reconstitution Instruction Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Applications Positive Control; Immunogen; SDS-PAGE; WB
Research Area Immunology
Target Function Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). Activated by the signaling pathway regulator NMI which acts as damage-associated molecular patterns (DAMPs) in response to cell injury or pathogen invasion, therefore promoting nuclear factor NF-kappa-B activation.
Subcellular Location Cell membrane; Single-pass type I membrane protein. Early endosome. Cell projection, ruffle.
Protein Family Toll-like receptor family
Associated Diseases The protein is encoded by the Lps locus, an important susceptibility locus, influencing the propensity to develop a disseminated Gram-negative infection.
Tissue Specificity Expressed in macrophages (at protein level). Highly expressed in heart, spleen, lung and muscle. Lower levels are found in liver and kidney.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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