Recombinant Mouse Thyrotropin Receptor (TSHR) Protein (His)

Name: Recombinant Mouse Thyrotropin Receptor (TSHR) Protein (His)
Brand: Beta LifeScience
SKU: BLC-11015P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Thyrotropin Receptor (TSHR) Protein (His) is produced by our Baculovirus expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P47750
Target Symbol	TSHR
Synonyms	Tshr; Thyrotropin receptor; Thyroid-stimulating hormone receptor; TSH-R
Species	Mus musculus (Mouse)
Expression System	Baculovirus
Tag	N-6His
Target Protein Sequence	KECASPPCECHQEDDFRVTCKELHRIPSLPPSTQTLKLIETHLKTIPSLAFSSLPNISRIYLSIDATLQRLEPHSFYNLSKMTHIEIRNTRSLTYIDPDALTELPLLKFLGIFNTGLRIFPDLTKIYSTDIFFILEITDNPYMTSVPENAFQGLCNETLTLKLYNNGFTSVQGHAFNGTKLDAVYLNKNKYLTAIDNDAFGGVYSGPTLLDVSSTSVTALPSKGLEHLKELIAKDTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSIRNLRQRKSVNILRGPIYQEYEEDPGDNSVGYKQNSKFQESPSNSHYYVFFEEQEDEVVGFGQELKNPQEETLQAFESHYDYTVCGDNEDMVCTPKSDEFNPCEDIMG
Expression Range	22-413aa
Protein Length	Partial
Mol. Weight	45.6
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism.
Subcellular Location	Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.
Protein Families	G-protein coupled receptor 1 family, FSH/LSH/TSH subfamily
Database References	KEGG: mmu:22095 STRING: 10090.ENSMUSP00000021346 UniGene: PMID: 28626114 Unlike its critical role in maintaining the normal growth and function of the thyroid gland, our results demonstrated that hepatic TSHR is involved in liver lipid metabolism and has little effect on energy metabolism. PMID: 29421660 Data indicate that splenic T cells from thyroid stimulating hormone receptor (TSHR) A-subunit primed animals undergoing Graves' orbitopathy (GO) showed proliferative responses to purified TSHR antigen and secreted proinflammatory cytokines. PMID: 26872090 Lung fibroblasts of TSHR KO mice have decreased cell surface Igf1r expression, and have Igf1r protein and protein fragments in both cytoplasm and nucleus. Igf1r mRNA levels were similar between TSHR KO and WT mice. PMID: 26389690 The mechanism, underlying TSH-induced liver triglyceride accumulation, involved that TSH, through its receptor TSHR, triggered hepatic SREBP-1c activity. PMID: 25016220 These findings suggest that activation of TSHR directly inhibits FASN expression in mature adipocytes, possibly mediated by PKA and ERK PMID: 25655684 A novel role for TSHR in behavioral and neurological phenotypes of Attention deficit/hyperactivity disorder. PMID: 25016105 The data indicate functional TSHR is expressed in ventricular myocytes and mediates TSH-induced BNP secretion and HMGCR up-regulation through the cAMP/PKA/pCREB signaling pathway. PMID: 24065308 findings demonstrate TSH-R expression is thymus-specific within the immune system; data support the notion of a novel neuroendocrine-immune interaction in which TSH-R signaling in the thymus, most likely mediated by TSH, enhances thymic T-cell development PMID: 24635198 The inhibition of hepatic PEPCK and G6P and enhanced expression of GK contributed to the development of fasting hypoglycemia in Tshr-ko mice. PMID: 23665701 UCP-1 and TSHR co-localized in retrobulbar adipose tissues in murine models of Graves' disease. PMID: 23386414 Cloned a thyroid-stimulating hormone receptor (TSHR) cDNA from thyroid glands. The sequence of this cDNA indicated that it encoded a 739 amino acid TSHR splice variant that lacked exon 5 (TSHR739). PMID: 23538203 Study with mouse monoclonal antibody 3BD10 confirms TSHR conformational heterogeneity and suggests that the N-terminal cysteine cluster may contribute to this structural variability. PMID: 23183178 Messenger RNA levels of hormone-sensitive lipase in epididymal fat containing the transferred TSHR gene were twofold higher than those in tissue from the control side PMID: 22496347 Data demonstrate the key role of TSH signaling in Braf-induced papillary thyroid cancer initiation and provide experimental support for observations in humans pointing to a strong association between TSH levels and thyroid cancer incidence. PMID: 21220306 Studies indicate it is important to refine knowledge and insights in non-autoimmune diseases caused by defects of the TSHR gene and it might help to develop pharmacological means for compensation of uncontrolled thyroid growth. PMID: 20515734 TSHR KO mice are, therefore, of value for future studies dissecting the autoimmune response to the mouse TSHR. PMID: 20555026 adipocyte TSHr under normal conditions affects adipocyte growth and development. PMID: 20152797 Consequences of hypothyroidism on auditory system function in Tshr mutant (hyt) mice. PMID: 11833606 Development of auditory brainstem responses (ABRs) in Tshr mutant mice derived from euthyroid and hypothyroid dams. PMID: 11833607 Knockout mice were used to study the role of the TSHR signaling in thyroid development & differentiation. The major role of the TSH/TSHR pathway is controlling genes involved in iodide metabolism. PMID: 12432093 TSHR expression was required for expression of sodium-iodide symporter but was not required for thyroglobulin expression, suggesting that the thyroid hormone synthetic pathway of the mouse could be dissociated into TSHR-dependent and -independent steps. PMID: 12432094 TSHR action is complemented by RII-PKAII which stably propagates robust cAMP signals in cell compartments PMID: 12902333 Ttf1 haploinsufficiency causes a reduction in the expression of TSHr and thyroglobulin, genes with TTF1 binding sites in their promoter regions PMID: 12907760 Pituitary folliculo-stellate cells express functional TSHR not coupled to cAMP nor IP3 but probably signals through JAK/STAT5a pathway. In vitro model to study role of TSHR in pituitary folliculo-stellate cells PMID: 15662590 Higher somatic mutations within complementarity-determining region 3 may correlate with pathogenic antibodies against TSH receptor. PMID: 17068139 Tshr(-/-) mice produced normal numbers of cones, indicating that the major requirement for thyroid hormone is in opsin patterning rather than in cone generation. PMID: 18974269 Melatonin activates Tshr expression in the pars tuberalis but not in the ependymal cell layer of the infundibular recess. PMID: 19589858

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Mouse Thyrotropin Receptor (TSHR) Protein (His)

Recombinant Mouse Thyrotropin Receptor (TSHR) Protein (His)

Product Overview

Target Details

FAQs