Recombinant Mouse Suppressor Of Cytokine Signaling 1 (SOCS1) Protein (His&Myc)

Name: Recombinant Mouse Suppressor Of Cytokine Signaling 1 (SOCS1) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-02790P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Suppressor Of Cytokine Signaling 1 (SOCS1) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	O35716
Target Symbol	SOCS1
Synonyms	Socs1; Cish1; Ssi1; Suppressor of cytokine signaling 1; SOCS-1; JAK-binding protein; JAB; STAT-induced STAT inhibitor 1; SSI-1
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MVARNQVAADNAISPAAEPRRRSEPSSSSSSSSPAAPVRPRPCPAVPAPAPGDTHFRTFRSHSDYRRITRTSALLDACGFYWGPLSVHGAHERLRAEPVGTFLVRDSRQRNCFFALSVKMASGPTSIRVHFQAGRFHLDGSRETFDCLFELLEHYVAAPRRMLGAPLRQRRVRPLQELCRQRIVAAVGRENLARIPLNPVLRDYLSSFPFQI
Expression Range	1-212aa
Protein Length	Full Length
Mol. Weight	28.7 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Through binding to JAKs and IFNGR1, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma (IFNG) mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway.
Subcellular Location	Nucleus. Cytoplasmic vesicle.
Protein Families	SOCS1 family
Database References	KEGG: mmu:12703 STRING: 10090.ENSMUSP00000038121 UniGene: PMID: 28079159 Feeding Guar gum fiber increases SOCS-1, TLR2 and dectin-1 expression in dextran sodium sulfate administered and normal mice. PMID: 28608623 SOCS1 attenuates migration and invasion properties of hepatocellular carcinoma cells at least partly via modulation of MET-mediated epithelial-mesenchymal transition, and controls invasive tumor growth. PMID: 29085209 Downregulated SOCS1 expression activates the JAK1/STAT1 pathway and promotes polarization of macrophages into M1 type. PMID: 28901399 these findings suggest that SOCS-1 may exert its protective effect in acute lung injury by rescuing epithelial sodium channel alpha-subunit expression via suppression of ASK-1. PMID: 27058411 loss of SOCS1 in CD11c+ cells skewed the balance of immune response to infection by increasing innate responses while decreasing antigen-specific adaptive responses to infectious antigens PMID: 29150567 MiRNA sponges against miR-19 or miR-155 inhibit the functions of these miRNAs and potentiate the induction of SOCS1 and p53 in mouse leukemia cells and in human myeloma cells. PMID: 26841929 IFN-lambda signaling is regulated by SOCS1 but not by SOCS3 or USP18. PMID: 28900038 Study conclude that under pro-proliferative cytokine stimulation at the onset of myeloproliferative diseases SOCS1 acts as a tumor suppressor, while under anti-proliferative conditions it exerts oncogenic function. PMID: 28753604 results indicate that SOCS1 is one of the essential molecules that maintain regulatory T cell stability, particularly under the inflammatory conditions in which APCs are highly activated PMID: 28550203 The Stat3 exerts its activity through the induction of miR- 155, which suppresses suppressor of cytokine signaling (SOCS)1. PMID: 27183599 miR-155 not only directly inhibited SOCS1 expression, but also increased the expression of p-STAT and PDCD4, as well as the production of proinflammation mediators IL-6 and TNF-alpha in atherogenesis. PMID: 27843203 TLR8 coupling with SOCS-1 inhibits TLR7-mediated antiviral immunity during WNV infection in mice. PMID: 27798161 nectin-4 serves as a stimulatory co-receptor for the prolactin receptor by regulating the feedback inhibition of SOCS1 in the JAK2-STAT5a signaling pathway PMID: 28258213 this study shows that genetic modification of dendritic cells with SOCS1 affects their activation and maturation, inhibits T-cell proliferation and induces hypo-responsiveness, and prolongs islet allograft survival PMID: 28215626 SOCS1 may promote apoptosis of intestinal epithelial cells at least partly through mediating p53 signaling. PMID: 27236107 Kdm5a associates with p50 and binds to the Socs1 promoter region in resting natural killer cells. PMID: 27050510 The miR-155 inhibitor relieves liver injury by enhancing the expression of SOCS1 and inactivating JAK/STAT signaling. PMID: 26251957 NOS1-derived nitric oxide promotes NF-kappaB transcriptional activity through inhibition of Socs1.Cys147 and Cys179 are essential for SOCS1 nitrosation and proteasomal degradation. PMID: 26324446 mir-155 deficiency attenuates liver ischemia-reperfusion injury through upregulation of SOCS1, which is associated with promotion of M2 macrophage and inhibition of Th17 differentiation PMID: 25611689 Findings indicate that SOCS1 mediates its tumor suppressor functions, at least partly, by binding to MET and interfering with downstream signaling pathways as well as by promoting the turnover of the activated MET receptor. PMID: 25728680 increased expression of SOCS-1 in the lungs of mice significantly protects against hyperoxic lung injury. PMID: 25850028 Overexpression of SOCS1 in transgenic rat eyes attenuated ocular HSV-1 infection PMID: 24993154 negative regulator of cell differentiation and phagocytosis in bone marrow-derived dendritic cells PMID: 25381480 Axl promotes STAT1 signaling via inhibition of SOCS1 in activated smooth muscle cells in vein graft remodeling. PMID: 26276821 targeting SOCS proteins, predominantly SOCS1, to suppress pathological mechanisms involved in atheroma plaque progression and destabilization could be an interesting anti-atherosclerotic strategy PMID: 25604439 While SOCS1 expression in adaptive immune cells is sufficient to inhibit antiviral immunity, SOCS1 in innate/stromal cells is responsible for aggravated lung injury. PMID: 25500584 miR-155 controls the outcome of the the germinal center reaction by modulating its initiation (Aicda) and termination (Socs1/p53 response). PMID: 25645925 these data imply that suppression of IFN-lambda signaling by virus-induced SOCS-1 causes an adaptive increase in IFN-lambda expression by host to protect cells against the viral infection PMID: 24391501 The Socs1 suppresses tumor formation in mammary glands by preventing the natural developmental function of a growth factor signaling pathway from becoming pro-oncogenic. PMID: 24037089 This study is the first to provide evidence that SOCS1 is involved in the regulation of neurogenesis. PMID: 24557021 These data demonstrate an important role for miR-155 in promoting staphylococcal enterotoxin b induced inflammation in the lungs through Socs1 suppression. PMID: 24778118 SOCS1 prevents GA progression by preserving endothelial function and attenuating cytokine-induced adhesion molecule expression in vascular endothelium. PMID: 23994402 These findings underscore the importance of SOCS1 in regulating potentially skin-reactive cytotoxic T lymphocytes, which could get activated under conditions that promote Ag-nonspecific, cytokine-driven proliferation. PMID: 23443260 These data indicate that IL-30 can provide critical protection against macrophage-mediated liver inflammation through anti-apoptotic, anti-oxidant, and anti-inflammatory activities PMID: 23583238 SOCS1 expression in DCs was down-regulated by 80% after Len-SOCS1-shRNA lentivirus infection. PMID: 23643168 Loss of SOCS-1 in the low-density lipoprotein receptor deficient murine model of atherosclerosis resulted in a complex, systemic and ultimately lethal inflammation. PMID: 23300554 miR-155 enhanced Treg and Th17 cells differentiation and IL-17A production by targeting SOCS1. PMID: 23091595 Progesterone suppresses TLRs-triggered immune response by regulating miR-155. The decreased miR-155 contributes to inhibit TLR-induced IL-6 and IFN-beta via increased SOCS1 expression. PMID: 22546503 miR-155 modulates TNF-alpha-regulated osteogenic differentiation by targeting SOCS1. PMID: 22634176 miR-155, an IFN-beta-induced miRNA, mediated the suppressive effect of IFN-beta on osteoclast differentiation by targeting SOCS1 and MITF, two essential regulators of osteoclastogenesis PMID: 22771905 High level of IL-10 within tumour microenvironment compromises Socs1-silenced dendritic cell vaccine functions. PMID: 22230748 Socs1 accelerates the onset of Flt3-internal tandem duplication - induced myeloproliferative disease by promoting the escape from external cytokine control. PMID: 22517899 Our findings suggest that SOCS1 DNA administration has considerable therapeutic potential in individuals with autoimmune myocarditis and dilated cardiomyopathy PMID: 22798678 SOCS1 is expressed via a new, NF-kappaB-independent pathway in Dectin-1-triggered murine bone marrow macrophages and influences Toll-like receptor cross-talk and T cell priming. PMID: 22547696 Toll-like receptor 2-mediated signalling is negatively regulated by SIGNR1-mediated signalling in dendritic cells, possibly through suppressor of cytokine signalling-1-mediated degradation of the MyD88-adaptor-like protein. PMID: 21899704 Socs1(-/-)Ifng(-/-) mice displayed accelerated liver regeneration with increased DNA synthesis compared to Ifng(-/-) and wild type mice. PMID: 21703184 results exemplify the negative role played by SOCS1 during T cell priming and effector functions during M. tuberculosis infection PMID: 21537342 miR-155 had a pro-inflammatory role in microglia and was necessary for the progression of the immune response through the modulation of SOCS-1. PMID: 22043967 SOCS1 is not only a feedback inhibitor of inflammation but also a critical molecular switch that tunes key signaling pathways to effectively program different sides of the macrophage balance PMID: 21628332

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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