Recombinant Mouse Somatostatin (SST) Protein (His-KSI)

Name: Recombinant Mouse Somatostatin (SST) Protein (His-KSI)
Brand: Beta LifeScience
SKU: BLC-00574P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Somatostatin (SST) Protein (His-KSI) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P60041
Target Symbol	SST
Synonyms	(NST)
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His-KSI
Target Protein Sequence	SANSNPAMAPRERKAGCKNFFWKTFTSC
Expression Range	89-116aa
Protein Length	Partial
Mol. Weight	18.5 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH; the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin.; May enhance low-glucose-induced glucagon release by pancreatic alpha cells. This effect may be mediated by binding to GPR107 and PKA activation. May regulate cardiac contractile function. May compromise cardiomyocyte viability. In the central nervous system, may impair memory retention and may affect hippocampal excitability. May also have anxiolytic and anorexigenic effects. May play a role in arterial pressure regulation. May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH.
Subcellular Location	Secreted.
Protein Families	Somatostatin family
Database References	KEGG: mmu:20604 STRING: 10090.ENSMUSP00000004480 UniGene: PMID: 30115649 Synapses from somatostatin-expressing interneurons are regulated by NMDA receptors, signaling through R-type calcium channels. PMID: 29295931 The findings reveal a proaddiction adaptive response to chronic nicotine in which nitric oxide and SST are released by a specific CHRNA5-expressing neuronal population to provide retrograde inhibition of the habenula- interpeduncular nucleus circuit and thereby enhance the motivational properties of nicotine. PMID: 29158387 Obesity- and gender-dependent role of endogenous somatostatin and cortistatin in the regulation of endocrine and metabolic homeostasis in mice. PMID: 27901064 HIV-1 Tat causes cognitive deficits and selective loss of parvalbumin, somatostatin, and neuronal nitric oxide synthase expressing hippocampal CA1 interneuron subpopulations. PMID: 27178324 Suppressing somatostatin (SOM) neurons enhanced the Excitation and Inhibition underlying size tuning, explaining SOM neurons' role in surround suppression. PMID: 28858618 dendrite-targeting somatostatin interneurons are critical for a visually induced, context-dependent gamma rhythm in visual cortex. PMID: 28481348 The results of this study provided the first in vivo evidence that SOM(+) neurons represent a Central Amygdala population that acquires learning-dependent sensory responsiveness during fear conditioning and furthermore reveal an important role of these neurons in gating passive versus active defensive behaviors. PMID: 27307236 preinspiratory Dbx1 neurons are rhythmogenic, inspiratory Dbx1+ and SST+ neurons primarily act to pattern respiratory motor output, and SST+-neuron-mediated pathways and postsynaptic inhibition modulate breathing pattern. I PMID: 27497222 Endogenous SST and CORT distinctly impact on DMBA-induced mammary gland tumorigenesis , in a manner that is strongly dependent on the metabolic/endocrine milieu (lean vs. obese status). PMID: 26956474 This study demonstrated that Hippocampal Somatostatin Interneurons Control the Size of Neuronal Memory Ensembles. PMID: 26875623 Neuronostatin acts via GPR107 to increase cAMP-independent PKA phosphorylation and proglucagon mRNA accumulation in pancreatic alpha-cells. PMID: 26561648 Data indicate that somatostatin (SST)-secretion is regulated by incretin hormones, cholecystokinin, acetylcholine, vasoactive intestinal polypeptide, calcitonin gene-related polypeptide, oligopetides, and trace amines. PMID: 26241122 Data indicate all gastric mucosa receptors through which somatostatin (SST) secretion is regulated by hormones, neurotransmitters, neuropeptides and metabolites that act directly on the SST cells. PMID: 26181106 The data of this study suggested that (1) low SST has a causal role in mood-related phenotypes, (2) deregulated EIF2-mediated protein translation PMID: 25600109 The PTEN autism spectrum disorder alleles are hypomorphic in regulating cell size and the parvalbumin/somatostatin ratio in comparison to WT PTEN. PMID: 25937288 Nearly all parvalbumin interneurons express Lynx1 did not detect Lypd6 in this population. Conversely, in somatostatin interneurons Lypd6 was found in a subset localized to deep cortical layers but no somatostatin neurons show detectable levels of Lynx1. PMID: 25359633 The paracrine actions of Ucn3 activate a negative feedback loop that promotes somatostatin release to ensure the timely reduction of insulin secretion upon normalization of plasma glucose. PMID: 26076035 Levels of TGF-beta1, a factor implicated in tissue remodelling, together with SMAD phosphorylation through which TGF-beta mediates its effects, were increased in the somatostatin knockout pancreas. PMID: 25350519 SOM regulates both parasite burden and granulomatous inflammation perhaps through modulating granuloma production of IFN-gamma and IL-1b PMID: 25530957 The role of somatostatin in the expression of growth hormone in male and female mice is reported. PMID: 25551181 Somatostatin and insulin together are critical paaracrine mediators of glucose-inhibited glucagon secretion and function by lowering cAMP/PKA signaling with increasing glucose. PMID: 25406263 Somatostatin-containing interneurons are involved in learning-induced changes in the inhibitory cortical network. PMID: 24055404 Impaired excitability of somatostatin- and parvalbumin-expressing cortical interneurons in a mouse model of Dravet syndrome. PMID: 25024183 SST, in a circadian manner and putatively via its regulation of expression in the amygdala, modulates behavior responding to mildly aversive conditions in mice. PMID: 24376834 Somatostatin-positive interneuron populations are altered by thyroid hormone deficiency. PMID: 24333174 when activated by SST-14, SSTR2A internalizes and recycles via the Golgi, which requires ECE-1 degradation of SST-14 and receptor dissociation from beta-arrestins PMID: 23913690 Findings suggest that somatostatin and its receptors (SSTR2 and SSTR5) are important markers in the regulation and development of Sertoli cell. PMID: 23831358 The results further support a role for endogenous SST in regulating l-Arg-mediated insulin release. PMID: 23696563 SST-like immunoreactivity, while comparable in most regions of hypothalamus, diminished significantly in arcuate nucleus of ApoD(-/-) mice. PMID: 22581439 Expression of somatostatin mRNA was increased in the hypothalamus in anorectic mice fed a Val-deficient diet. PMID: 21293891 Nkx2.2-/-Arx- ghrelin+ cells also express the somatostatin hormone PMID: 21880149 the role of endogenous CST is different from SST; CST exerted an unexpected stimulatory role on prolactin secretion PMID: 21971153 Data show that gastric infection and inflammation is associated with increased IFNgamma expression and reduced ghrelin expression, and that IFNgamma does not directly control ghrelin expression but inhibits it indirectly via somatostatin. PMID: 21912454 Data from studies on islets of Sst knockout mice suggest that paracrine interactions between delta- and alpha-cells provide intra-islet Sst-mediated paracrine communication that facilitates release of glucagon in response to sympathetic activation. PMID: 21099335 investigation of role of somatostatin and somatostatin receptors in sexually dimorphic pattern of growth hormone expression in fasting/fed states; studies in hypothalamus, pituitary, and stomach; studies with male and female SST-knockout mice PMID: 20943754 Somatostatin secretion is greatly facilitated by cell-to-cell interactions and E-cadherin expression. PMID: 20637727 Data show that approximately 95% of mouse olfactory bulb somatostatin-immunoreactive cells describe a homogeneous population of interneurons. PMID: 20394054 Extraovarian somatostatin, acting through its receptors 2 and 5 present on granulosa cells, may be involved in mouse folliculogenesis by reducing recruitment of resting follicles. PMID: 20056831 opposite and stage-specific effects on the migration of cerebellar granule cells PMID: 11780120 Somatostatin acts as a selective chemoattractant for immature hematopoietic cells PMID: 11823046 substance P and somatostatin are temporally expressed in granulomas associated with murine cysticercosis, which may be related to differential expression of Th1 and Th2 cytokines PMID: 12117965 Brain somatostatin receptors 1,2,4 and 5 are up-regulated in somatostatin-deficient mice, and SSTR3 is down-regulated. PMID: 12145348 adrenomedullin, acting via intramural fundic neurons, stimulates somatostatin and thus inhibits histamine and acid secretion PMID: 12573799 Somatostatin regulates ductal bile formation in mice by inhibition of ductal fluid secretion, also by stimulation of ductal fluid absorption via interacting with SSTR2 on cholangiocytes, a process involving the intracellular cAMP/cGMP second messengers. PMID: 12676656 In whole area of hippocampus, NPY-, SOM- (somatostatin), CCK-, and VIP-positive neurons accounted for about 31%, 17%, 7%, and 8% of GABAergic neurons, respectively. PMID: 12746872 Infrequent colocalization of nNOS and somatostatin in mouse hippocampus. Double-labeled cells may be particular subpopulation of hippocampal GABAergic nonprincipal neurons. PMID: 15026120 In embryonic kidneys, SRIF is expressed first at the interface of the metanephric mesenchyme and basolateral ureteric bud and later in maturing thin descending limbs of Henle. Expression in the thin descending limb persists in the adult kidney. PMID: 15496149 In the hippocampus of a transgenic Alzheimer's disease model at 6 months of age, the expression of somatostatin (SOM) and NPY neuropeptides displayed a significant decrease PMID: 16271420 Somatostatin inhibits oxidative respiration in pancreatic beta-cells PMID: 16357046

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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