Recombinant Mouse Sodium/Potassium-Transporting Atpase Subunit Alpha-1 (ATP1A1) Protein (His)

Name: Recombinant Mouse Sodium/Potassium-Transporting Atpase Subunit Alpha-1 (ATP1A1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-06850P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Sodium/Potassium-Transporting Atpase Subunit Alpha-1 (ATP1A1) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q8VDN2
Target Symbol	ATP1A1
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	TVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWFALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIEVCCGSVMEMREKYSKIVEIPFNSTNKYQLSIHKNPNASEPKHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLLLPDEQFPEGFQFDTDDVNFPVDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVNQVNPRDAKACVVHGSDLKDMTSEELDDILRYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIVGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKS
Expression Range	341-775aa
Protein Length	Partial
Mol. Weight	53.3 kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Subcellular Location	Basolateral cell membrane; Multi-pass membrane protein. Cell membrane, sarcolemma; Multi-pass membrane protein. Cell projection, axon. Melanosome.
Protein Families	Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily
Database References	KEGG: mmu:11928 STRING: 10090.ENSMUSP00000039657 UniGene: PMID: 28181111 Cardiotonic steroids activate NF-kappaB leading to proinflammatory cytokine production in primary macrophages through a signaling complex, including CD36, TLR4, and Na/K-ATPase. PMID: 28619997 Study identified the Na+/K+-ATPase alpha 1 and 3 subunits as receptors for the extracellular fragment of GPNMB that mediates activation of cellular signaling pathways and subsequent neuroprotective effects. PMID: 26988030 effects of endogenous acetylcholine on alveolar fluid clearance are likely mediated by Na,K-ATPase function through activation of muscarinic acetylcholine receptors on alveolar epithelia PMID: 26134533 Oxidized LDL-bound CD36 recruits an Na/K-ATPase-Lyn complex in macrophages that promotes atherosclerosis. PMID: 26350901 TGFbeta1 is a powerful regulator of megakaryocytic Na+/K+ ATPase activity. PMID: 25168590 Inhibition of the Na+/K+-ATPase pathway in astrocytes leads to rearrangement of cytoskeleton. PMID: 24095695 Reduction of Na/K-ATPase affects cardiac remodeling and increases c-kit cell abundance in partial nephrectomized mice. PMID: 24748592 Enhanced Na+ entry did not alter Na,K-ATPase a1 mRNA level in tranfected mCDD cells. Post-transcriptional control of Na,K-ATPase abundance is effected by lysosomal degradation. PMID: 24179170 This coupling of astrocytic A2ARs to the regulation of glutamate transport through modulation of NKA-alpha2 activity provides a novel mechanism linking neuronal activity to ion homeostasis controlling glutamatergic activity. PMID: 24259572 Concurrent impairment of Na(+)+K(+)-ATPase function in multi-organ may serve as one of the molecular pathways participating in and contributing to the mechanism of type-1 diabetes-induced complications in NOD mice. PMID: 22906552 Activation of Na,K-ATPase protects against oxidative damage in an animal model of pentylenetetrazol-induced seizures. PMID: 23219656 The results reveled that the NKAalpha1 subunit is found within alpha-motoneurons of spinal cord. PMID: 23761886 Reduction of Na+, K+-ATPase activity and expression in cerebral cortex of glutaryl-CoA dehydrogenase deficient mice is a possible mechanism for brain injury in glutaric aciduria type I. PMID: 22999741 Results indicate that Activation of Na+,K+-ATPase in skeletal muscle cells is AMPK-dependent. PMID: 22610379 the sodium pump alpha1 sub-unit has a role in progression of metastatic melanoma PMID: 19243476 ATP1A1 interaction with PKCeta and occludin was involved in the integrity of the blood-labyrinth-barrier PMID: 21304972 Both protein and mRNA expression of alpha1 and alpha2 isoforms of Na,K-ATPase and NKCC1 in the lateral wall were dramatically reduced following a long-term deafening PMID: 21094218 Functional coupling between the vascular alpha2 Na(+) pump and NCX1 is a critical molecular mechanism for salt-induced blood pressure elevation. (review) PMID: 21048395 Konjac polysaccharide could decrease body weight, postprandial blood glucose, serum leptin and intestinal mucosa Na -K+-ATPase activity of mice treated with high fat. PMID: 19408668 Alpha(1) isoform is expressed at more constant levels in both diaphragm and hindlimb muscles. PMID: 12397396 Mouse embryos homozygous for a null mutation in the Na/K-ATPase alpha1-subunit gene are able to undergo compaction and cavitation PMID: 15147760 results demonstrate that the Na,K-ATPase alpha 1 isoform regulates cardiac contractility and is functionally and physically coupled with the Na/Ca exchanger in heart PMID: 15485817 identification of groups of genes whose expressions were perturbed in the alpha1 heterozygous hearts compared to wild-type PMID: 15706699 The results indicate that the Na(+)/K(+)-ATPase is a potent regulator of tight junction formation and function during mouse preimplantation development. PMID: 16356488 identified a well conserved Na,K-ATPase catalytic alpha-subunit motif that binds to the inositol 1,4,5-trisphosphate receptor and can trigger an anti-apoptotic calcium signal. PMID: 16723354 Tubuloglomerular feedback requires activity of the alpha1 Na,K-ATPase, presumably in the macula densa. Furthermore, the data show that neither NHE2 nor colonic H,K-ATPase is essential for initiation of TGF responses[Na,K-ATPase alpha1] PMID: 16870707 while ATP1A3-isoforms regulate sodium and potassium homeostasis in subicular interneurones, ATP1A1-isoforms assume this function in pyramidal cells PMID: 17947306 This study provides a new link between alteration of sodium pump activity and hypertension in vivo, and suggests that TCTP might be a therapeutic target for the treatment of hypertension. PMID: 17976639 studies of the behavioral aspects of alpha1, alpha2, and alpha 3 deficient mice show that haploinsufficiency of both alpha2 and alpha 3 isoforms result in behavioral defects. PMID: 18044013 Examine roles of NCX1 and Atp1a1 in phospholamban regulation of cardiomyocyte contractility. PMID: 18708446

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.