Recombinant Mouse Serine Protease Inhibitor Kazal-Type 1 (SPINK1) Protein (GST)
Beta LifeScience
SKU/CAT #: BLC-10041P

Greater than 90% as determined by SDS-PAGE.
Recombinant Mouse Serine Protease Inhibitor Kazal-Type 1 (SPINK1) Protein (GST)
Beta LifeScience
SKU/CAT #: BLC-10041P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Mouse Serine Protease Inhibitor Kazal-Type 1 (SPINK1) Protein (GST) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | P09036 |
Target Symbol | SPINK1 |
Synonyms | Spink1; Spink3; Serine protease inhibitor Kazal-type 1; P12; Prostatic secretory glycoprotein; Serine protease inhibitor Kazal-type 3 |
Species | Mus musculus (Mouse) |
Expression System | E.coli |
Tag | N-GST |
Target Protein Sequence | AKVTGKEASCHDAVAGCPRIYDPVCGTDGITYANECVLCFENRKRIEPVLIRKGGPC |
Expression Range | 24-80aa |
Protein Length | Full Length of Mature Protein |
Mol. Weight | 33.1kDa |
Research Area | Others |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | Serine protease inhibitor which exhibits anti-trypsin activity. In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.; In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production. |
Subcellular Location | Secreted. |
Database References | |
Tissue Specificity | In the genital tract, expressed only in male accessory glands including seminal vesicle, coagulating gland and prostate. |
Gene Functions References
- It is a trypsin-specific inhibitor in the pancreas. PMID: 24521862
- IFN regulatory factor 2 (Irf2) has a regulatory role in trypsinogen5 gene transcription, which is resistant to a major endogenous trypsin inhibitor, Spink3 PMID: 22042864
- SPINK3 modulates sperm physiology through a downstream reduction of endogenous NO concentration and independently of SPINK3 trypsin inhibitory activity. PMID: 22228629
- uterine-gland-derived SPINK3, as a new paracrine modulator, might play an important role in embryo implantation through its influence on stromal decidualization in mice. PMID: 20623140
- Spink3 may act as a growth factor, leading to cell proliferation and regeneration of tissue structure. PMID: 20660988
- Results suggest that regulation of Prss1 and Spink3 expression is involved in the susceptibility to experimentally induced pancreatitis. PMID: 20157294
- Spink3 trypsin inhibitor is required for normal pancreatic development. Data indicate an important relationship between endogenous pancreatic trypsin and Spink3 and the development of pancreatitis. PMID: 20110462
- SPINK3 mRNA is upregulated in chronic pancreatitis. PMID: 15097871
- Spink3 may play important roles in proliferation and/or differentiation of various cell types during development. PMID: 18386042