Recombinant Mouse Semaphorin-4D (SEMA4D) Protein (mFc), Active

Name: Recombinant Mouse Semaphorin-4D (SEMA4D) Protein (mFc), Active
Brand: Beta LifeScience
SKU: BLC-05817P
Availability: InStock

Greater than 95% as determined by SDS-PAGE.

Activity Measured by its binding ability in a functional ELISA. Immobilized Pepinemab at 2 μg/ml can bind Mouse Sema4d, the EC 50 is 4.878-7.446 ng/ml. Biological Activity Assay

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Product Overview

Description	Recombinant Mouse Semaphorin-4D (SEMA4D) Protein (mFc), Active is produced by our Mammalian cell expression system. This is a protein fragment.
Purity	Greater than 95% as determined by SDS-PAGE.
Endotoxin	Less than 1.0 EU/ug as determined by LAL method.
Activity	Measured by its binding ability in a functional ELISA. Immobilized Pepinemab at 2 μg/ml can bind Mouse Sema4d, the EC 50 is 4.878-7.446 ng/ml.
Uniprotkb	O09126
Target Symbol	SEMA4D
Synonyms	(M-Sema G)(Semaphorin-C-like 2)(Semaphorin-J)(Sema J)(CD100)
Species	Mus musculus (Mouse)
Expression System	Mammalian cell
Tag	C-mFc
Target Protein Sequence	FAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSGTSCEPKMVINTVPQLHSEKTVYLKSSDNR
Expression Range	24-733aa
Protein Length	Partial
Mol. Weight	108.1 kDa
Research Area	Immunology
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.
Subcellular Location	Cell membrane; Single-pass type I membrane protein.
Protein Families	Semaphorin family
Database References	KEGG: mmu:20354 STRING: 10090.ENSMUSP00000021900 UniGene: PMID: 27218883 our findings revealed for the first time that Sema4D positively regulated both the adaptive and innate immune responses in contact hypersensitivity PMID: 27614265 SEMA4D-/- neutrophils show impaired NET formation when in contact with endothelial cells. PMID: 28416516 Absence of Sema4D improves oligodendrocyte recovery after cerebral ischemia/reperfusion injury in mice PMID: 26752319 Sema4D-deficiency affected ischemia-induced microglial activation in morphology, iNOS expression, and proliferation, and this deficiency most likely resulted in moderation of cytotoxicity and locomotor behavioral abnormality after cerebral ischemia PMID: 26202989 The addition of recombinant Sema4D to Sema4D/ vaginal epithelial cells in culture significantly enhanced apoptosis of the vaginal epithelial cells. PMID: 25351707 Sema4D may be an essential apoptosis-inducing ligand that acts downstream of estrogen action in vaginal epithelium during pubertal tissue remodeling. PMID: 24841081 therapeutic effects via bone-targeting systems featuring interference of Sema4d are able to partly counteract alveolar bone loss caused by osteoporosis. PMID: 25252878 CD100-mediated signals are critical for effective activation of gammadelta IEL to produce growth factors, including KGF-1, that are required for healing of the colon epithelium during colitis. PMID: 23695512 Deletion of plexin-B1, the high affinity receptor for semaphorin 4D, significantly (one-way ANOVA; p < 0.001) reduced thrombus weight (- 40%) in oxidative venous thrombosis. PMID: 23642655 Data indicate neuroimmune semaphorin 4D (Sema4D) as an important regulator of Th2-driven lung pathophysiology and as a potential target for a combinatory disease immunotherapy. PMID: 23911404 Sema 4D/CD100 is not only a ligand for plexin B1 but also a direct signaling receptor in mediating Sema 4D/CD100-plexin B's biological functions. PMID: 23262975 CD100 deficiency in vivo resulted in delayed repair of cutaneous wounds due to a disrupted gamma-delta T cell response to keratinocyte damage. PMID: 22902232 Sema4D may act as an intrinsic inhibitory regulator of oligodendrocyte differentiation by promoting apoptosis. PMID: 22198439 in vivo, Sema4D is an indirect regulator of bone resorption, which acts via its effect on reproductive function PMID: 22046317 Data show that osteoclasts express semaphorin 4D (Sema4D), previously shown to be an axon guidance molecule, which potently inhibits bone formation. PMID: 22019888 Studies identify Plxdc1, Sema4D, and neuropilin-1 as novel LRP1-regulated cell-signaling proteins. PMID: 20919742 Loss of Semaphorin 4D is associated with defect in collagen-induced platelet aggregation and an impaired vascular injury response. PMID: 20855865 Findings suggest that Sema4D is involved in the progression phase of atherosclerosis by accelerating intimal neovascularization, resulting in enhanced macrophage infiltration in atherosclerotic plaques. PMID: 20514420 Sema4D enhances inducible nitric oxide synthase (iNOS) and nitric oxide (NO) production in microglia. PMID: 20038643 Sema4D-plexin-B2 interactions regulate mechanisms underlying cell specification, differentiation, and migration during corticogenesis. PMID: 19948886 Sema4D is involved in oligodendrogenesis during development and during recovery from ischemic injury. PMID: 19472224 CD100, which enhances the maturation of DCs, is essential in the activation and differentiation of Ag-specific T cells. PMID: 12133937 Semaphorin 4D triggers invasive growth, a complex programme that includes cell dissociation, anchorage-independent growth and branching morphogenesis. PMID: 12198496 enhances the in vitro activation and differentiation of T cells and the in vivo generation of antigen-specific T cells PMID: 12374982 Sema4D is a novel inhibitory factor for axonal regeneration expressed in myelin PMID: 14534257 Sema4D may function as a neurite-outgrowth stimulating factor in embryonic sensory neurons. PMID: 15330859 Strength of B-cell antigen receptor signals is strictly tuned by the interaction of CD100 with CD72; this interaction is essential for maintaining immunological homeostasis as well as generating a proper immune response. PMID: 16113236 CD100 enhances pathogenetic humoral immune responses and promotes the activation of Antigen-Presenting Cells by up-regulating CD86 expression PMID: 16920982 proteolytic cleavage of Semaphorin 4D may provide a novel molecular mechanism by which membrane type 1-matrix metalloproteinase controls tumor-induced angiogenesis PMID: 17204469 semaphorin 4D has a role in platelet responses to vascular injury PMID: 17244710 In the ovarian follicle, the effect of Plexin-B1 is mediated by sema-4D. PMID: 17376242 Sema4D modulates T cell-APC interactions in allogeneic responses both in vivo and in vitro. PMID: 17950916 that tumor-associated macrophages (TAMs) are the main cells producing Sema4D within the tumor stroma and that their ability to produce Sema4D is critical for tumor angiogenesis and vessel maturation. PMID: 18559453 A functional role for CD100 antigen/Plxnb1 interactions in epithelial branching morphogenesis during organogenesis is reported. PMID: 18799546 loss of sema4D (semaphorin 4D) expression reduces the platelet hyperactivity otherwise found in dyslipidemia, and confers protection against the development of atherosclerosis PMID: 19390055 These results suggest that SHP2-mediated tyrosine dephosphorylation is an important step in Sema4D-induced axon repulsion. PMID: 19433062 these findings suggest a novel role of Sema4D/CD100 in the regulation of microglia activation providing a valuable neuroprotective tool PMID: 19467284 Mouse behavioral analyses revealed enhanced motor activity in Sema4D-deficient mice, suggesting the crucial involvement of Sema4D in the neurodevelopmental processes of the central structures mediating motor behavior in mice. PMID: 19534337

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.