Recombinant Mouse Sclerostin Domain-Containing Protein 1 (SOSTDC1) Protein (His&Myc)

Name: Recombinant Mouse Sclerostin Domain-Containing Protein 1 (SOSTDC1) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-02431P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse Sclerostin Domain-Containing Protein 1 (SOSTDC1) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q9CQN4
Target Symbol	SOSTDC1
Synonyms	Sostdc1; Sostl; Usag1; Sclerostin domain-containing protein 1; Ectodermal BMP inhibitor; Ectodin; Sclerostin-like protein; Uterine sensitization-associated gene 1 protein; USAG-1
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	FKNDATEILYSHVVKPVPAHPSSNSTLNQARNGGRHFSSTGLDRNSRVQVGCRELRSTKYISDGQCTSISPLKELVCAGECLPLPVLPNWIGGGYGTKYWSRRSSQEWRCVNDKTRTQRIQLQCQDGSTRTYKITVVTACKCKRYTRQHNESSHNFESVSPAKPAQHHRERKRASKSSKHSLS
Expression Range	24-206aa
Protein Length	Full Length of Mature Protein
Mol. Weight	27.6 kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	May be involved in the onset of endometrial receptivity for implantation/sensitization for the decidual cell reaction. Enhances Wnt signaling and inhibits TGF-beta signaling. Directly antagonizes activity of BMP2, BMP4, BMP6 and BMP7 in a dose-dependent manner.
Subcellular Location	Secreted.
Protein Families	Sclerostin family
Database References	KEGG: mmu:66042 STRING: 10090.ENSMUSP00000040230 UniGene: PMID: 27102547 suggest that RUNX2 and USAG-1 act in an antagonistic manner PMID: 27518316 the in vivo inter-relationships between Bmp7 and Usag-1, was examined. PMID: 27178071 Findings strongly suggest that Wise and Sost are key modulators of bone development through the ability of their encoded proteins to interact with Lrp5 and control the balance or levels of Wnt signaling. PMID: 24789067 Interactions between BMP-7 and USAG-1 (uterine sensitization-associated gene-1) regulate supernumerary organ formations PMID: 24816837 Sost and its paralog Sostdc1 coordinate digit number in a Gli3-dependent manner. PMID: 23994639 Wise controls the number and distribution of the mammary epithelial cells via inhibition of Wnt/beta-catenin signaling. PMID: 23293290 Data suggest that Sostdc1 primarily regulates bone morphogenetic protein pathway in pancreatic islets; knockout/mutation of Sostdc1 enhances down-regulation of Ctgf (connective tissue growth factor) and gremlin in islets after high-fat diet. PMID: 22829579 The data demonstrate that simvastatin contributes to prevent the progression of renal fibrosis by upregulating BMP-7-mediated anti-fibrotic signaling and that one aspect of crucial efficacies is achieved by regulating HOXA13 and USAG-1. PMID: 22525429 The data suggested that functions of Sostdc1 can be largely attributed to its ability to attenuate Wnt/beta-catenin signaling. PMID: 22509524 analyses demonstrate that the Fgf and Shh pathways are major downstream targets of Wise-regulated Wnt signaling. PMID: 20724449 the pathogenetic role of USAG-1 in Col4a3-/- mice might involve crosstalk between kidney tubules and the glomerulus and that inhibition of USAG-1 may be a promising therapeutic approach for the treatment of Alport syndrome. PMID: 20197625 Data suggest that ectodin is a novel bone morphogenetic protein (BMP) inhibitor which integrates BMP signaling with the SHH and FGF signal pathways PMID: 14623234 reported that ectodin, a secreted bone morphogenetic protein (BMP) inhibitor, is expressed as a "negative" image of enamel knots; proposed that ectodin is critical for robust spatial delineation of enamel knots and cusps PMID: 16179481 Uterine sensitization-associated gene-1 (USAG-1) plays a critical role in the modulation of renoprotective action of bone morphogenetic protein. PMID: 16341262 Expression of USAG-1 mRNA appears to be associated with blastocyst implantation to the luminal epithelium, suggesting that physiological or biochemical contact of the blastocyst to the uterus is required for USAG-1 expression PMID: 17389776 USAG-1 controls the number of teeth in the maxillary incisor region by regulating apoptosis PMID: 17555714 Enhanced BMP signaling results in supernumerary teeth and BMP signaling was modulated by Wnt signaling in the USAG-1 deficient mouse model. PMID: 18329379 minimizing the amount of dental mesenchyme in cultured Sostdc1-deficient incisors causes the formation of additional de novo incisors that resemble the successional incisor development that results from activated Wnt signaling. PMID: 19141669

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.