Recombinant Mouse Platelet Glycoprotein Vi (GP6) Protein (His-SUMO)

Name: Recombinant Mouse Platelet Glycoprotein Vi (GP6) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-08073P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Platelet Glycoprotein Vi (GP6) Protein (His-SUMO) is produced by our E.coli expression system. This is a extracellular protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P0C191
Target Symbol	GP6
Synonyms	Gp6; Platelet glycoprotein VI; GPVI; Glycoprotein 5
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	QSGPLPKPSLQAQPSSLVPLGQSVILRCQGPPDVDLYRLEKLKPEKYEDQDFLFIPTMERSNAGRYRCSYQNGSHWSLPSDQLELIATGVYAKPSLSAHPSSAVPQGRDVTLKCQSPYSFDEFVLYKEGDTGSYKRPEKWYRANFPIITVTAAHSGTYRCYSFSSSSPYLWSAPSDPLVLVVTGLSATPSQVPTEESFPVTESSRRPSILPTNKISTTEKPMNITASPEGLSPPFGFAHQHYAK
Expression Range	22-265aa
Protein Length	Extracellular Domain
Mol. Weight	43.0 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Collagen receptor involved in collagen-induced platelet adhesion and activation. Plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcR gamma-chain, the Src kinases (likely FYN or LYN) and SYK, the adapter protein LAT and leads to the activation of PLCG2.
Subcellular Location	Cell membrane; Single-pass membrane protein.
Database References	KEGG: mmu:243816 STRING: 10090.ENSMUSP00000104231 UniGene: PMID: 29269852 These results reveal a novel and unexpected function of hepatic Fc-gamma-RIIB in the targeted downregulation of GPVI in vivo. PMID: 27297794 collagen-I-mediated inhibition of proplatelet formation is specifically controlled by GPVI. PMID: 27505889 platelet GP6 and thromboxane A2 receptor have a role in promoting inflammatory macrophage phenotype in skin inflammation PMID: 27818280 Novel antithrombotic peptides derived from trowaglerix that acts through GPVI antagonism with greater safety-no severe bleeding. PMID: 28596377 TULA-2 Protein Phosphatase Suppresses Activation of Syk through the GPVI Platelet Receptor for Collagen by Dephosphorylating Tyr(P)346, a Regulatory Site of Syk. PMID: 27609517 Inhibition of platelet activation by an anti-GPVI antibody significantly reduces infarct size. PMID: 26916731 These results demonstrate that GPVI is a receptor for fibrin and provide evidence that this interaction contributes to thrombus growth and stability. PMID: 26282541 data demonstrate that genetic deletion of GPVI receptor, FcRgamma chain, or the alpha2beta1 integrin changes the thrombotic potentials of these platelets to collagen dependent on the stimulus mechanism. PMID: 25415203 data suggest a novel role for FAK in GPVI-dependent ROS formation and platelet activation and elucidate a proximal signaling role for FAK within the GPVI pathway. PMID: 25415317 our results show that GPVI plays a dual role in inflammation by enhancing neutrophil-damaging activities while supporting the activation and hemostatic adhesion of single platelets to neutrophil-induced vascular breaches. PMID: 26036804 This study identifies GPVI as a platelet receptor for polymerized fibrin with 2 major functions: (1) amplification of thrombin generation and (2) recruitment of circulating platelets to clots. PMID: 25977585 Functional studies of platelets from Ceacam2(-/-)-deficient mice (Cc2(-/-)) revealed that CEACAM2 serves to negatively regulate collagen glycoprotein VI (platelet) (GPVI)-FcRgamma-chain and the C-type lectinlike receptor 2 (CLEC-2) signaling PMID: 25085348 Glaucocalyxin A inhibits platelet activation and thrombus formation preferentially via GPVI signaling pathway. PMID: 24386454 a delayed and markedly reduced thrombogenic response was still evident in VWF(-/-), GPVI, and alpha2beta1 blocked animals, suggesting that alternative primary hemostatic mechanisms can partially rescue the bleeding phenotype associated with these defects. PMID: 25051961 RhoG is expressed and activated in platelets, plays an important role in GPVI-Fc receptor gamma-chain complex-mediated platelet activation, and is critical for thrombus formation in vivo. PMID: 24106269 demonstrate that isolated targeting of either GPVI or CLEC-2 in vivo does not affect expression or function of the respective other receptor PMID: 23448972 Data show an inhibitory function of CLP-36 in GPVI immunoreceptor tyrosine-based activation motif signaling and as a key regulator of arterial thrombosis. PMID: 22955732 Recombinant GPVI-Fc immunoglobulin fragment binds to activated vascular endothelium and prevents platelet/endothelial interaction. PMID: 22814400 Cdc42 is required for platelet filopodia formation, secretion and aggregation and therefore plays a critical role in platelet mediated hemostasis and thrombosis. PMID: 21789221 A model in which PECAM-1/SHP-2 complexes, formed in a Lyn-dependent manner, suppress GPVI signaling. PMID: 21297004 PECAM-1-mediated inhibition of GPVI-dependent platelet responses result from recruitment of SHP-2-p85 complexes to tyrosine-phosphorylated PECAM-1, which diminishes the association of PI3K with activatory signaling molecules Gab1 and LAT PMID: 20723025 Glycoprotein 6 cleavage in vitro can occur independently through either ADAM10 or ADAM17 in response to distinct stimuli. PMID: 20644114 Ablation of TULA-2 resulted in hyperphosphorylation of Syk and its downstream effector phospholipase C-gamma2 as well as enhanced platelet glycoprotein VI-mediated platelet functional responses. PMID: 20585042 The presence of platelet GPVI facilitates experimental tumor metastasis but does not contribute to the growth of primary tumors. PMID: 19624454 the GPVI receptor utilizes a unique intracellular proline-rich domain (PRD) to accelerate platelet activation, a requirement for efficient platelet adhesion to collagen under flow PMID: 19940238 c-Cbl negatively regulates platelet responses to GpVI agonists and to thrombin, with the latter effect possibly being mediated downstream of GpIIb/IIIa PMID: 14629478 Platelet mitochondrial injury induced rapid proteolytic cleavage of GPVI and GPIb; platelet stimulation with thrombin or CRP, however, resulted in marked metalloproteinase-dependent shedding of GPIbalpha, but not GPVI. PMID: 15116256 studies establish platelet-collagen responses under physiologic flow as the consequence of a close partnership between 2 structurally distinct receptors, glycoprotein VI and integrin alpha2beta1 PMID: 15886326 study demonstrates a minor role for the p110delta catalytic subunit in mediating platelet activation by the collagen receptor GPVI and integrin alphaIIbeta3 PMID: 16011964 These observations clearly establish that blockade of GPVI may attenuate platelet-collagen interactions without adversely affecting the bleeding time. PMID: 16139873 GPVI is a novel receptor for laminin and support a model in which integrin alpha6beta1 brings laminin to GPVI. PMID: 16219796 Platelet activation by thrombin appears to be more important after laser injury than platelet activation by GPVI-collagen. PMID: 16455953 Absence of GP Ibalpha function has a more profound antithrombotic effect in vivo than absence of the GP VI-dependent pathway of collagen-induced adhesion/activation PMID: 16961609 activation of phospholipase C gamma 2 via GPVI is dependent on 2 complementary events PMID: 17579183 one or more modifier genes in Mh control the extent to which in vivo platelet thrombus formation is disrupted by the absence of platelet GPVI PMID: 17991808 Globular adiponectin induces platelet activation through the collagen receptor GPVI-Fc receptor gamma chain complex PMID: 18419742 In mice, GPVI-mediated platelet adhesion to the atherosclerotic vascular wall is involved in atheroprogression in vivo. PMID: 18431526 GPVI-Fc preferentially bound to sites of vascular injury and was able to inhibit neointima formation after wire-induced vascular injury in ApoE(-/-) mice. PMID: 18566102 Gads plays a key role in linking the adapter LAT to SLP-76 in response to weak activation of GPVI and CLEC-2 whereas LAT is required for full activation over a wider range of agonist concentrations. PMID: 18826392 demonstrate a reciprocal relationship in levels of the novel PKC isoforms delta and epsilon in human and mouse platelets and a selective role for PKCepsilon in signalling through GPVI. PMID: 19030108 PI3Kbeta plays an essential role in GPVI-mediated platelet aggregation and Akt activation PMID: 19700402

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.