Recombinant Mouse Phospholipid Transfer Protein (PLTP) Protein (His)

Name: Recombinant Mouse Phospholipid Transfer Protein (PLTP) Protein (His)
Brand: Beta LifeScience
SKU: BLC-06360P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Phospholipid Transfer Protein (PLTP) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P55065
Target Symbol	PLTP
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	ELPGCKIRVTSAALDLVKQEGLRFLEQELETITIPDVYGAKGHFYYNISDVRVTQLHLISSELHFQPDQDLLLNISNASLGLHFRRQLLYWFLYDGGYINASAEGVSIRTGLQLSQDSSGRIKVSNVSCEASVSKMNMAFGGTFRRMYNFFSTFITSGMRFLLNQQICPVLYHAGTVLLNSLLDTVPVRSSVDDLVGIDYSLLKDPVVSNGNLDMEFRGAFFPLKEDNWSLPNRAVEPQLEDDERMVYVAFSEFFFDSAMESYFQAGALQLTLVGDKVPSDLDMLLRATYFGSIVLLSPTVINSPLKLKLEATSPPRCTIKPSGTTISITASVTITLAPPMLPEVELSKMIMEGRLSAKLTLRGKALRVKLDLRRFQIYSNQSALESLALIPLQAPLKTLLQIGVMPLLNERTWRGVQIPLPEGINFVREVVTNHAGFVTVGADLHFAKGLREVIDKNRPADVAASHVPPPSAAAA
Expression Range	18-493aa
Protein Length	Full Length of Mature Protein
Mol. Weight	56.7 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Mediates the transfer of phospholipids and free cholesterol from triglyceride-rich lipoproteins (low density lipoproteins or LDL and very low density lipoproteins or VLDL) into high-density lipoproteins (HDL) as well as the exchange of phospholipids between triglyceride-rich lipoproteins themselves. Facilitates the transfer of a spectrum of different lipid molecules, including sphingomyelin, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, and phosphatidyl ethanolamine. Plays an important role in HDL remodeling which involves modulating the size and composition of HDL. Also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer phosphatidylcholine from phospholipid vesicles to HDL, and an inactive form that lacks this capability.
Subcellular Location	Secreted. Nucleus.
Protein Families	BPI/LBP/Plunc superfamily, BPI/LBP family
Database References	KEGG: mmu:18830 STRING: 10090.ENSMUSP00000061519 UniGene: PMID: 26320740 these findings elucidated that PLTP repressed LPS induced inflammation due to extracellular LPS binding capability, and the protective effects were not related to HDL pool size in mice. PMID: 26857615 Data presented a novel model to link phospholipid metabolism to APP processing and also suggested that PLTP played an important role in Abeta metabolism and would be useful to further elucidate functions of PLTP in Alzheimer's disease susceptibility. PMID: 26160914 In hepatocytes, initial recruitment of phospholipid (PL) by apoB:1000 leading to the formation of the PL-rich apoB-containing initiation complex is mediated to a large extent by PLTP. PMID: 25638820 PLTP plays an important role in modulating the stability of atherosclerotic plaques. The receptor-interacting protein 3- reactive oxygen species signal pathway could be involved in this PLTP-mediated process. PMID: 25324570 Adipocyte PLTP plays a small but significant role in plasma PLTP activity and promotes cholesterol efflux from adipose tissues. PMID: 25477345 PLTP exerts significant effects on apoA-I lipidation and nascent HDL biogenesis in hepatocytes by promoting ATP-binding cassette transporter A1-mediated lipid efflux and the remodeling of nascent HDL particles. PMID: 25060793 Plasma Sphingosine-1-phosphate contents were decreased by 60.1 % in PLTP knockout mice (PLTP-/-, N = 5) compared with their wild type littermates (WT, N = 5) (151.70 +/- 38.59 vs. 379.32 +/- 59.90 nmol/l, P<0.01). PMID: 24158769 Therefore we concluded that PLTP deficiency impaired cognition and aggravated AD by enhancing the generation of Abeta in the cortex of old mice. PMID: 24121956 Cerebrovascular oxidative stress increased in PLTP deficient mice, including increased levels of reactive oxygen species (ROS) and lipid peroxidation marker 4-hydroxy-2-nonenal (HNE) and reduced superoxide dismutase (SOD) activity. PMID: 24513285 PLTP is actively involved in lipid transfer, cholesterol efflux, HDL genesis, and remodeling at the blood-brain barrier. PMID: 24369175 PLTP can play a significant role in the pathophysiology of abdominal aortic aneurysm. PMID: 23830874 Liver-specific PLTP deficiency significantly reduces plasma HDL and apoB-containing lipoprotein levels. PMID: 23846500 The results of this study suggested that PLTP, through its ability to deliver vitamin E to the brain, constitutes an endogenous neuroprotective agent. Increasing PLTP activity may offer a new way to develop neuroprotective therapies. PMID: 23303044 Our results suggest, for the first time, that the major function of liver PLTP is to drive VLDL production and makes a small contribution to plasma PLTP activity. PMID: 22367708 presence of PLTP in tear fluid appears to be essential for maintaining a healthy and functional ocular surface PMID: 21514421 the SR-BI pathway contributes in unique ways to cholesterol metabolism and atherosclerosis susceptibility even in the presence of CETP PMID: 21454568 Diet-induced lipid accumulation in phospholipid transfer protein-deficient mice: its atherogenicity and potential mechanism PMID: 20543142 PLTP increases blood coagulation and worsens the extent of ischemic lesions in response to acute oxidative stress. PMID: 20864671 It is concluded that PLTP is essential in mediating the association of triacyl lipid A with lipoproteins, leading to extension of its residence time and to magnification of its proinflammatory and anticancer properties. PMID: 20418497 increased PLTP permits triglyceride incorporation into abnormally large VLDL, which are removed from plasma by LDL receptors PMID: 20037162 data show that PLTP regulates the bioavailability of vitamin E in atherogenic lipoproteins and suggest a novel strategy for achieving more effective concentrations of anti-oxidants in lipoproteins, independent of dietary supplementation PMID: 12105225 pltp is regulated by liver X receptors in mice PMID: 12177004 elevated PLTP activity in human cholesteryl ester transfer protein (huCEPT) transgenic mice results in an increase in VLDL secretion PMID: 12401886 The phospholipid transfer protein gene is a liver X receptor target expressed by macrophages in atherosclerotic lesions. PMID: 12612088 Two-fold increased plasma PLTP activity in apoE knockout mice results in a decrease in HDL cholesterol, HDL phospholipid, and apoAI levels and indicates that elevated plasma PLTP levels constitute a novel, long-term risk factor for atherosclerosis. PMID: 12855484 PLTP has a role in transporting cholesterol and phospholipids from cells to lipoprotein particles by a process involving PLTP interactions with cellular ATP-binding cassette transporter A1 PMID: 14559902 Five significant loci were identified as being involved in PLTP activity in the mouse and supporting evidence was provided for the candidacy of Nr1h4 and Apof as the genes underlying Pltpq2. PMID: 14592843 LDL from PLTP0 mice was markedly resistant to oxidation and induced significantly less monocyte chemotactic activity compared with that in controls. In vitro, PLTP0 HDL removed significantly more oxidized phospholipids from LDL than did control HDL PMID: 15258196 PLTP deficiency decreases liver vitamin E content, increases hepatic oxidant tone, and substantially enhances reactive oxygen species-dependent destruction of newly synthesized apolipoprotein B via a post-endoplasmic reticulum process. PMID: 15734742 data suggested that PLTP may play important role(s) during in vivo preimplantation embryo development PMID: 15832314 The effect of PLTP gene deletion on the fertility of male and female mice is reported. PMID: 16467369 complex regulatory role of PLTP in serum and liver lipid homeostasis, as well as in the formation of nascent apoA-I-PL complexes from the liver PMID: 16554055 Absence of endogenous phospholipid transfer protein impairs ABCA1-dependent efflux of cholesterol from macrophage foam cells. PMID: 16687660 Macrophage PLTP deficiency causes a significant reduction of apoE secretion from the cells, and this in turn promotes the accumulation of cholesterol in the circulation and accelerates the development of atherosclerosis. PMID: 17038631 PLTP deficiency in knockout mice results in reduced cholesterol uptake as well as secretion by the intestine. PMID: 17641249 antiatherogenic properties of macrophage-derived PLTP are related at least in part to its ability to reduce cholesterol accumulation in macrophages through changes in the alpha-tocopherol content and oxidative status of the cells PMID: 17717294 unlike total systemic PLTP, locally produced macrophage-derived PLTP beneficially alters lipoprotein metabolism and reduces lesion progression in hyperlipidemic mice PMID: 17928634 PLTP appears to play a proatherogenic role in diet-induced hyperlipidemic mice PMID: 18198166 PLTP expression by bone marrow derived cells results in atherogenic effects on plasma lipids, increased PLTP activity, high local PLTP protein levels in the atherosclerotic lesions and increased atherosclerotic lesion size PMID: 18509527 PLTP transfer activity is essential for the development of atherosclerosis in PLTP transgenic mice. PMID: 18711210 These findings indicate that plasma HDL level is an important regulator of plasma PLTP and might play a role in the stabilization of PLTP in plasma. PMID: 19422933 Elevated PLTP activity lowers plasma HDL-cholesterol by stimulating the plasma turnover and hepatic uptake of HDL cholesteryl esters. PMID: 19524061

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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