Recombinant Mouse Nuclear Receptor Subfamily 5 Group A Member 2 (NR5A2) Protein (His&Myc)

Name: Recombinant Mouse Nuclear Receptor Subfamily 5 Group A Member 2 (NR5A2) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-11056P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Nuclear Receptor Subfamily 5 Group A Member 2 (NR5A2) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P45448
Target Symbol	NR5A2
Synonyms	Nr5a2; Lrh1; Nuclear receptor subfamily 5 group A member 2; Liver receptor homolog 1; LRH-1
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MSASLDTGDFQEFLKHGLTAIASAPGSETRHSPKREEQLREKRAGLPDRHRRPIPARSRLVMLPKVETEAPGLVRSHGEQGQMPENMQVSQFKMVNYSYDEDLEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNQKRYTCIENQNCQIDKTQRKRCPYCRFKKCIDVGMKLEAVRADRMRGGRNKFGPMYKRDRALKQQKKALIRANGLKLEAMSQVIQAMPSDLTSAIQNIHSASKGLPLSHVALPPTDYDRSPFVTSPISMTMPPHSSLHGYQPYGHFPSRAIKSEYPDPYSSSPESMMGYSYMDGYQTNSPASIPHLILELLKCEPDEPQVQAKIMAYLQQEQSNRNRQEKLSAFGLLCKMADQTLFSIVEWARSSIFFRELKVDDQMKLLQNCWSELLILDHIYRQVAHGKEGTIFLVTGEHVDYSTIISHTEVAFNNLLSLAQELVVRLRSLQFDQREFVCLKFLVLFSSDVKNLENLQLVEGVQEQVNAALLDYTVCNYPQQTEKFGQLLLRLPEIRAISKQAEDYLYYKHVNGDVPYNNLLIEMLHAKRA
Expression Range	1-560aa
Protein Length	Full Length
Mol. Weight	71.5 kDa
Research Area	Developmental Biology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38.
Subcellular Location	Nucleus.
Protein Families	Nuclear hormone receptor family, NR5 subfamily
Database References	KEGG: mmu:26424 STRING: 10090.ENSMUSP00000027649 UniGene: PMID: 29662071 potent suppressor of neural stem cell self-renewal and key player in neural fate decisions through direct regulatory effects on critical genes and pathways PMID: 27447294 transcriptional regulation by NR5A2 links differentiation and inflammation in the pancreas; findings support the notion that, in the pancreas, the transcriptional networks involved in differentiation-specific functions also suppress inflammatory programs; under conditions of genetic or environmental constraint, these networks can be subverted to foster inflammation PMID: 29443959 The data identify the key role of biliary phospholipids in sustaining intestinal mucosa proliferation and tumor progression through the activation of nuclear receptor Lrh1. PMID: 27995969 It was concluded that Nr5a2 is essential for cumulus expansion in granulosa cells throughout follicular development. PMID: 28520915 These findings suggest that compromised SUMOylation of LRH-1 promotes the development of nonalcoholic fatty liver disease under lipogenic conditions through regulation of OSBPL3. PMID: 28094767 these data show for the first time LRH-1 expression in T cells, its role in FASLG transcription and the potential of pharmacological inhibition of LRH-1 in the treatment of FasL-mediated immunopathologies PMID: 28406481 highlight the importance of LRH-1 in coordinating glutamine-induced metabolism and signaling to promote hepatocellular carcinogenesis. PMID: 27298334 Mice carrying a mutation on lysine 289 of LRH-1 (Lrh1 K289R mice) display reduced LRH-1 SUMOylation and increased expression of genes regulating cholesterol transport. PMID: 25176150 Results identify LRH-1 as a critical component of the anti-inflammatory and fungicidal response of alternatively activated macrophages that acts upstream from the IL-13-induced 15-HETE/PPARgamma axis. PMID: 25873311 The authors conclude that LRH-1 initiates a novel pathway of endoplasmic reticulum stress resolution that is independent of the unfolded protein response, yet equivalently required. PMID: 24737860 study demonstrates for the first time that LRH-1 has a CRT-dependent NES which is not only required for cytoplasmic trafficking, but also essential for correct protein folding to avoid misfolding-induced aggregation. PMID: 26268559 these data demonstrate that copper-mediated nuclear receptor dysfunction disrupts liver function in WD and potentially in other disorders associated with increased hepatic copper levels. PMID: 26241054 Heterozygous gene deletion of LRH-1 causes body weight gains without any apparent worsening of glucose and lipid metabolism. PMID: 24531913 NR5A2 has a role in controlling aspects of multipotent progenitor cell formation and acinar differentiation during pancreatic organogenesis PMID: 25063451 Report fully reversible infertility phenotype of LRH-1-knockdown mice. PMID: 23425349 this study shows that the combination of Rarg and Nr5a2 rapidly promote the iN cell maturation within 1 week and greatly facilitate the conversion with neuronal purities of approximately 50% and yields of >130%. PMID: 24459144 Data (including data from transgenic overexpression/gene silencing) suggest that Nr5a2 modulates signal transduction/cell proliferation in mammary cells; mammary morphology exhibits significant reduction in lateral budding after Nr5a2 overexpression. PMID: 24564400 Data (including data from various knockout mice strains) suggest that Nr5a2 differentially regulates female fertility across the trajectory of follicular development especially in later stages of follicle development. PMID: 24552399 FTF combined with CYP7A might play an important role in Lrh1 regulated pancreas-specific transcriptional network. PMID: 24735206 regulates pancreatic acinar plasticity and constrains pancreatic neoplasia driven by oncogenic Kras PMID: 23645620 NR5A2 can act in a non-redundant manner to regulate Lhb and Fshb transcription in vitro, but is dispensable in vivo. PMID: 23536856 Lrh-1 is necessary for maintenance of the corpus luteum, for promotion of decidualization and for formation of the placenta. PMID: 23817023 Data indicate that sequence divergence has differentially impacted ligand binding and protein dynamics in NR5A2. PMID: 23737522 Liver receptor homolog-1 (LRH-1), a member of the nuclear receptor superfamily, is expressed in kisspeptin neurons in the arcuate nucleus but not in the anteroventral periventricular in female mice. PMID: 23504956 in conditional deletion of Lrh1 in liver, analysis of hepatic glucose fluxes revealed reduced glucokinase and glycogen synthase fluxes as compared with those of wild-type littermates PMID: 22772466 LRH-1 recruits FXR to lipid metabolic genes. PMID: 22296850 findings demonstrate that signaling through RARs has critical roles in molecular reprogramming and that the synergistic interaction between Rarg and Lrh1 directs reprogramming toward ground-state pluripotency PMID: 21990348 Data show that LRH-1 is a key regulator of the exocrine pancreas-specific transcriptional network required for the production and secretion of pancreatic fluid. PMID: 21852532 Liver receptor homolog-1 is critical for adequate up-regulation of Cyp7a1 gene transcription and bile salt synthesis during bile salt sequestration. PMID: 21391220 findings identify an LRH-1 dependent phosphatidylcholine signalling pathway that regulates bile acid metabolism and glucose homeostasis PMID: 21614002 Data characterize two functional nuclear localization signals in LRH-1 and show that three crucial basic clusters are involved in the DNA binding and transcriptional activities of LRH-1. PMID: 20853131 These data indicate that LRH-1 and Nanog cooperate to regulate Dax1 expression in mouse embryonic stem cells. PMID: 20937355 through interaction with LRH-1 and transcriptional activation of Oct4 and other genes PMID: 20943815 Lrh-1 was identified as a novel beta-catenin target gene, and Lrh-1 regulation is required for maintaining proper levels of Oct4, Nanog, and Tbx3. PMID: 20734354 show that the orphan nuclear receptor Nr5a2 (also known as Lrh-1) can replace Oct4 in the derivation of iPSCs from mouse somatic cells, and it can also enhance reprogramming efficiency. PMID: 20096661 role in mediating species- and cell line-specific bile acid-dependent negative feedback regulation of the apical sodium-dependent bile acid transporter PMID: 12456679 the specificities of LXXLL-related motifs in Dax-1 based on their amino acid sequences play an important role in regulation of orphan receptors. PMID: 12482977 up-regulation of Lrh-1 and Mrp3(Abcc3) reduces liver injury in obstructive cholestasis PMID: 12837754 LRH-1 was expressed in estrogen biosynthesis-granulosa cells during the estrous cycle, and in corpora lutea of pregnancy and was proposed playing an important role as a competence factor in regulating aromatase and estrogen biosynthesis in ovary. PMID: 12972182 The FTF-beta galactosidase mouse establishes a central role for FTF in developmental, nutritive, and metabolic functions from early embryogenesis through adulthood. PMID: 15014077 Results show that LRH-1 induces cell proliferation through the concomitant induction of cyclin D1 and E1, an effect that is potentiated by its interaction with beta-catenin. PMID: 15327767 LRH-1 plays an essential role in the maintenance of Oct4 expression in ES cells at the epiblast stage of embryonic development. PMID: 15831456 Roles in gonadal development, the initiation of folliculogenesis and regulation of estrogen biosynthesis within the ovary. PMID: 16003771 LRH-1 is strongly expressed throughout the mouse brain suggesting important roles for this transcription factor PMID: 16264403 FTF and LRH-1 are two related but different transcription factors in human Caco-2 cells, suggesting that they may be homologues and not orthologues. PMID: 16469397 mlrh-1v2 expression is directed by a new promoter which displays a strong activity in ES cells PMID: 16500646 LRH-1 is a new transcriptional partner for GATA factors in the regulation of inhibin alpha-subunit gene expression. PMID: 16893604 These results demonstrate a novel role for liver receptor homologue-1 (LRH-1) in the regulation of intestinal glucocorticoid synthesis and propose LRH-1 as an important regulator of intestinal tissue integrity and immune homeostasis. PMID: 16923850 LRH-1 is a key player in the control of the hepatic acute-phase response. PMID: 16943422

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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