Recombinant Mouse Natriuretic Peptides B (NPPB) Protein (His&Myc)

Name: Recombinant Mouse Natriuretic Peptides B (NPPB) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-07310P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse Natriuretic Peptides B (NPPB) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P40753
Target Symbol	NPPB
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	YPLGSPSQSPEQFKMQKLLELIREKSEEMAQRQLLKDQGLTKEHPKRVLRSQGSTLRVQQRPQNSKVTHISSCFGHKIDRIGSVSRLGCNALKLL
Expression Range	27-121aa
Protein Length	Full Length of Mature Protein
Mol. Weight	18.3 kDa
Research Area	Cardiovascular
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis.
Subcellular Location	[Brain natriuretic peptide 45]: Secreted.
Protein Families	Natriuretic peptide family
Database References	KEGG: mmu:18158 STRING: 10090.ENSMUSP00000099521 UniGene: PMID: 29734386 The present study provides a novel insight into the potential underlying mechanisms of telmisartan-induced inhibition of cardiomyocyte hypertrophy, which involves inhibition of NFAT activation, nuclear translocation and the ANP/BNP cascade. PMID: 28447738 the developmental regulation and stress response of the Nppa-Nppb cluster involve the concerted action of multiple enhancers and epigenetic changes distributed across a structurally rigid regulatory domain. PMID: 27048739 Data suggest that the negative inhibition of P2X3R activity by the BNP/NPR-A pathway results in a decreased P2X3R-mediated excitability of trigeminal neurons in wildetype cultures. In familial hemiplegic migraine type-1 model cultures, however, lack of efficient P2X3Rs downregulation contributes to the neuronal hyperexcitability phenotype. PMID: 27346147 P2X3 receptors on mouse trigeminal ganglion neurons are subjected to contrasting modulation by inhibitory brain natriuretic peptide and facilitatory calcitonin gene-related peptide that both operate via complex intracellular signaling. PMID: 27175010 mouse trigeminal neurons endogenous BNP acts on NPR-A receptors to determine constitutive depression of P2X3 receptor function. Tonic inhibition of P2X3 receptor activity by BNP/NPR-A/PKG pathways occurs via two distinct mechanisms: P2X3 serine phosphorylation and receptor redistribution to non-raft membrane compartments PMID: 26576636 Data (including data from studies in knockout mice) suggest expression of ANP/BNP/GC-A (atrial natriuretic peptide, brain natriuretic peptide, and guanylyl cyclase-A) is important in embryonic neovascularization and organogenesis. PMID: 26517044 Goalpha plays a role in ANF sorting during intracellular vectorial transport and with the presence of a mechanism that preserves the molar relationship between cellular ANF and BNP. PMID: 25917834 The RNA-Seq demonstrates that NPPB which well activates natriuretic peptide receptor 1 (NPR1), is well expressed in mouse dorsal root ganglian. PMID: 25123163 a slow modulatory action by BNP on TRPV1 and P2X3 receptors outlining the role of this peptide as a negative regulator of trigeminal sensory neuron excitability to nociceptive stimuli PMID: 24312267 These data provide detailed novel insight into the complex effects of natriuretic peptides and their receptors on electrical conduction in the heart. PMID: 24344164 Natriuretic peptides buffer renin-dependent hypertension. PMID: 24717731 BNP-NPRA signaling is involved in both itch and pain and does not function upstream of the GRP-GRPR dedicated neuronal pathway. PMID: 24438367 Data indicate that the identified stress-responsive enhancer of Nppa and Nppb, the most common markers of heart failure, is a 650-bp fragment within 50 kb of the Nppa and Nppb loci. PMID: 24391132 these results define the primary pruriceptive neurons, characterize Nppb as an itch-selective neuropeptide, and reveal the next two stages of this dedicated neuronal pathway. PMID: 23704570 Elevated BNP levels, by inducing sympathetic overdrive and altering Ca(2+) handling, promote adverse cardiac remodelling and ventricular arrhythmias PMID: 22617407 BNP controls early load-dependent regulation of SERCA through calcineurin in heart failure. PMID: 20711735 Expression of brain natriuretic peptide and other serum response factor-dependent fetal cardiac genes in response to acute mechanical stress was blunted in mice lacking Mrtf-A. PMID: 20606005 plasma levels of adrenomedullin, atrial natriuretic peptide(ANP)and BNP increased in association with aging; ANP and BNP increased in association with pulse pressure; possible relation between levels and age-related changes in cardiovascular system PMID: 12484513 BNP-mRNA expression in normotensive AT2-deficient mice demonstrate a direct interaction of the renin-angiotensin system and natriuretic peptide that is fibrosis- rather than blood pressure-dependent. PMID: 14599720 activation of cardiac GC-A by locally secreted atrial and brain natriuretic peptides protects the heart from excessive cardiac remodeling by inhibiting the calcineurin-NFAT pathway. PMID: 15939815 ET-1 and Ang II play a key role in the induction of B-type natriuretic peptide in response to load in intact adult murine hearts. PMID: 17188849 BNP is a specific HIF-1alpha target gene PMID: 17822384 mephrin A initially truncated BNP in kidney and the truncated BNP was catabolized by neprilysin PMID: 17823376 The results show that B-type natriuretic peptide decreases intragastric pressure, emptying and absorption by acting via the NPR-A receptor. PMID: 18367637 BNP promotes vessel growth by increasing the number of endothelial progenitors and enhancing their functional properties. PMID: 19275472 endogenous ANP/BNP protects the heart against the death and progression of pathological remodeling in a mouse model of dilated cardiomyopathy and sudden death PMID: 19346456 Targeting of BNP with short interfering RNA (siRNA) resulted in the inhibition of embryonic stem cell proliferation, as indicated by a marked reduction in the cell number and colony size. PMID: 19399180 Data indicate that EET-mediated cardioprotection involves BNP and PI3K signalling events. PMID: 19401302 The natriuretic peptide/guanylyl cyclase--a system functions as a stress-responsive regulator of angiogenesis in mice. PMID: 19487812 Data show that the system was able to measure the induction of hypertrophic markers BNP and ANP in tissue after 2 hrs and 5 hrs of stretch. PMID: 19670825

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.