Recombinant Mouse Mucosal Addressin Cell Adhesion Molecule 1 (MADCAM1) Protein (His-SUMO)

Name: Recombinant Mouse Mucosal Addressin Cell Adhesion Molecule 1 (MADCAM1) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-09424P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse Mucosal Addressin Cell Adhesion Molecule 1 (MADCAM1) Protein (His-SUMO) is produced by our E.coli expression system. This is a extracellular protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q61826
Target Symbol	MADCAM1
Synonyms	Madcam1; Mucosal addressin cell adhesion molecule 1; MAdCAM-1; mMAdCAM-1
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	QSFQVNPPESEVAVAMGTSLQITCSMSCDEGVARVHWRGLDTSLGSVQTLPGSSILSVRGMLSDTGTPVCVGSCGSRSFQHSVKILVYAFPDQLVVSPEFLVPGQDQVVSCTAHNIWPADPNSLSFALLLGEQRLEGAQALEPEQEEEIQEAEGTPLFRMTQRWRLPSLGTPAPPALHCQVTMQLPKLVLTHRKEIPVLQSQTSPKPPNTTSAEPYILTSSSTAEAVSTGLNITTLPSAPPYPKLSPRTLSSEGPCRPKIHQDLEAGWELLCEASCGPGVTVRWTLAPGDLATYHKREAGAQAWLSVLPPGPMVEGWFQCRQDPGGEVTNLYVPGQVTPNSSS
Expression Range	22-364aa
Protein Length	Extracellular Domain
Mol. Weight	52.8kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Database References	STRING: 10090.ENSMUSP00000020554 UniGene: Mm.391556
Associated Diseases	Absence of Madcam1 in the spleen has been found in aly/aly mice, but normal expression is found in intestinal venules. This aberrant expression is a secondary defect and not the direct cause of aly alymphoplasia, an autosomal recessive mutation which induces total aplasia of lymph nodes and Peyer patches.
Tissue Specificity	Highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues like the Peyer patches and mesenteric lymph nodes, and in the lamina propria of the intestine. Some expression found in the spleen, and low levels of expression in

Gene Functions References

Nkx2-3 deficiency reprograms the endothelial addressin preference for lymphocyte homing in Peyer's patches. PMID: 25320278
findings indicate that alpha4beta7*IgG can be used as a probe for functional MAdCAM-1 expressed on HEVs in GALT and could potentially serve as an anti-inflammatory drug inhibiting GALT-specific lymphocyte migration PMID: 21430257
ectopic expression of MAdCAM-1 at the BBB does not increase alpha4beta7-integrin-mediated immune cell trafficking into the CNS during MOG(aa35-55)-induced experimental autoimmune encephalomyelitis PMID: 21341265
Observed a significant decrease in expression of ICAM-1 and MAdCAM-1 mRNA/protein in lactobacillus plantarum treated colitis. PMID: 19960256
results suggest that AT1R regulates the expression of MAdCAM-1 under colonic inflammatory conditions through regulation of the translocation of NF-kappaB into the nucleus PMID: 19940029
Lymphocyte binding to MAdCAM-1 via alpha4beta7 integrin activates a signal transduction pathway involving tyrosine phosphorylation of paxillin and p105(Cas-L) PMID: 11947929
Mucosal addressin cell adhesion molecule 1 plays an unexpected role in the development of mouse guard hair. PMID: 12230506
spatial heterogeneity of MAdCAM-1 and VCAM-1 activation following TNF-alpha challenge may promote specific T lymphocyte recruitment in the inflamed colonic mucosa PMID: 12388196
Increased expression of mucosal addressin cell adhesion molecule-1 in gastritis induced by Helicobacter pylori PMID: 12390304
expression of MAdCAM-1 by fibroblasts and melanomas suggests MAdCAM-1 may play a role in regulating host responses in the periphery, leucocyte transmigration across nonendothelial boundaries, or the homotypic interactions of some malignant melanomas PMID: 12848854
GST-3 is responsible for MECA-79 (MADCAM1)defined luminal ligands PMID: 15111310
MAdCAM-1 plays important roles in T-lymphocyte adherence in all intestinal sites. PMID: 15371130
In a chronic ileitis model, pathogenic CD4+ T cells use the MAdCAM-1/alpha4beta7 physiologic pathway in order to recirculate to the chronically inflamed small intestine. PMID: 15699171
In wild-type mice administered blocking monoclonal antibody (mAb) to VCAM1-1 but nor to Madcam1 recruitment of mast cells to the inflamed lung was not reduced. PMID: 16670268
These data indicate that TNF-alpha deficiency alters homoeostasis of the colonic chemokine/cytokine environment and humoral immune response, resulting in an exacerbation of acute DSS-induced colitis in TNF-alpha(-/-) mice. PMID: 17768420
Combined effects of diabetes and pregnancy on pancreatic and decidual expression of VCAM-1, MAdCAM-1 and PNAd. PMID: 18031809
Expression of MAdCAM-1 in isolated lymphoid follicles (ILFs) of the small intestine colocalizes with CD31 expression, indicating that MAdCAM-1 expression in ILFs is restricted to vascular structures. PMID: 18768861

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.